The assay of insulin solutions by paper chromatography

Abstract

1961aldehyde. Vitamin A1 aldehyde was the predominant form in all except the freshwater perch, pike, and trout and the anadromous salmon, which contained predominantly vitamin A2 aldehyde.3. Values are given for the conteiut of total vitamin A1 and A2 esters and alcohols, the percentage of the A2 form and the carotenoid content of the eggs or ovaries.We are grateful to the many people who have helped with the collection of samples, and thank Dr L. R. Fisher for his help in zoological matters and Mr C. M. Ward for technical assistance.

The incorporation of tritium-labelled amino acids into insulins in rat pancreas in vitro

“…It contained insulin and other proteins, and also some salt and fatty material which might have interfered with the subsequent purification. The saline and fatty impurities were easily removed by the following procedure (Fenton, 1961). The precipitate was dissolved in 4-5 ml.…”

Actions of Insulin and Growth Hormone on Collagen and Chondroitin Sulfate Synthesis in Bone Organ Cultures

“…), a gift of Eli Lilly and Co., Indianapolis, Indiana, U.S.A.; crystalline bovine zinc insulin (24.2 U./ mg.), a gift of Commonwealth Serum Laboratories (CSL), Melbourne, Australia; amorphous bovine insulin prepared from the CSL product by the picrate precipitation method. 26 The basic medium level of insulin was 0.001 U. per milliliter. Amersham, England).…”