The Arthus Reaction in Rodents: Species-Specific Requirement of Complement

Szalai, Alexander J.; Digerness, Stanley B.; Agrawal, Alok; Kearney, John F.; Bucy, R. P.; Niwas, Shri; Kilpatrick, J. Michael; Babu, Y.S.; Volanakis, John E.

doi:10.4049/jimmunol.164.1.463

Cited by 56 publications

(44 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…45 We have not examined the impact of complement activation in our model, which might have resulted in even greater efficacy, although the complement pathway in mice is somewhat different from rats. 44,46,47 After gaining access to the brain parenchyma, the ability of G47D to replicate specifically in tumor cells and spread throughout the tumor is apparent from continued labeling of the tumor for at least 19 days after injection. We have previously detected extensive X-gal staining of G207-infected human subcutaneous tumors at 15 and 24 days after treatment, 5,48 while others have detected infectious HSV up to 31 days postinfection.…”

Section: Oncolytic Hsv Therapy Of Brain Tumors After Bbbd R Liu Et Almentioning

confidence: 99%

Intracarotid delivery of oncolytic HSV vector G47Δ to metastatic breast cancer in the brain

2005

View full text Add to dashboard Cite

Delivery of viral vectors to tumors in the brain is a challenge, especially via systemic administration, which is key to targeting the invasive margins of malignant glioma and the multiple foci of metastatic disease. Like for other cancer therapeutics, the blood-brain barrier or even the bloodtumor barrier significantly limits delivery and efficacy. Bloodbrain barrier disruption (BBBD) is one strategy for transiting the cerebrovasculature. G47D is a third-generation oncolytic replication-competent herpes simplex virus (HSV) vector, containing deletions of the g34.5 and a47 genes and an inactivating LacZ insertion in UL39 (ICP6). Intracarotid artery delivery of G47D after BBBD with 25% mannitol significantly extended the life of nude mice bearing intracerebral human MDA-MB-435 breast tumors, whereas, G47D injection contralateral to the tumor, in the absence of mannitol or mannitol alone had no effect on survival. G47D replication was extensive after BBBD, as visualized by X-gal staining. Staining of peripheral organs, lung and liver, was minimal and not altered by BBBD. This is the first demonstration of intracarotid arterial delivery of oncolytic HSV vectors and antitumor efficacy in a mouse model and opens the door to the use of mouse syngenic tumor models and transgenic/ knockout animals. Gene Therapy (2005) 12, 647-654.

show abstract

Section: Oncolytic Hsv Therapy Of Brain Tumors After Bbbd R Liu Et Almentioning

confidence: 99%

Intracarotid delivery of oncolytic HSV vector G47Δ to metastatic breast cancer in the brain

2005

View full text Add to dashboard Cite

show abstract

“…injection of 25 g/animal of cobra venom factor (Quidel) in PBS 18 h before bacterial challenge. This procedure was previously shown to reduce levels of immunodetectible C3 to less than 3% of normal and result in a period of hypocomplementemia of greater than 48 h (27).…”

Section: Neutrophil and Complement Depletionmentioning

confidence: 99%

Mucosal Clearance of Capsule-Expressing Bacteria Requires Both TLR and Nucleotide-Binding Oligomerization Domain 1 Signaling

Zola

Лысенко

Weiser

2008

The Journal of Immunology

View full text Add to dashboard Cite

Expression of capsular polysaccharide by bacterial pathogens is associated with increased resistance to host clearance mechanisms, in particular by evading opsonization and uptake by professional phagocytes. The potential for rapid progression of disease caused by encapsulated bacteria points to the importance of innate immunity at the mucosal surface where infection is initiated. Using a murine model of nasopharyngeal colonization, host immune components that contribute to the mucosal clearance of capsule-expressing bacteria were investigated. Clearance of encapsulated Haemophilus influenzae (Hi) required both TLR and nucleotide-binding oligomerization domain (NOD) signaling pathways, whereas individual deficiencies in each of these signaling cascades did not affect clearance of nonencapsulated strains. Moreover, clearance of Hi-expressing capsular polysaccharide required the recruitment of neutrophils to the site of infection, and ex vivo phagocytic bacterial killing required expression of the NOD1 signaling pathway. Conversely, redundancies within these innate immune pathways of non-neutrophil cells were sufficient to promote mucosal clearance of nonencapsulated Hi. Our findings reveal a role for NOD1 in protection from encapsulated pathogens. In addition, this study provides an example of a microbial virulence determinant that alters the requirements for host signaling to provide effective protection.

show abstract

“…Plant extracts or plant-derived compounds are under investigation in order to accomplish these purposes. In these efforts, a number of screening tests can be used in rodents, including the immune complex-induced reversed passive Arthus reaction (Bailey and Sturm, 1983;Szalai et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Anti-Inflammatory Capacity of Beta-Sitosterol in Rodent Assays

Paniagua-Pérez¹,

Flores-Mondragón²,

Reyes-Legorreta³

et al. 2016

AJTCAM

View full text Add to dashboard Cite

Background: Beta-sitosterol (BS) is a compound discovered to be present in numerous plants. A number of interesting biomedical properties have been attributed to BS, including immuno-modulating and anti-inflammatory activities. Therefore, the aim of this report was to evaluate its anti-inflammatory capacity by applying various rodent experimental tests. Methods. To carry out the objective of the study we applied the methods indicated here. Two of the adopted methods were based on the passive reverse Arthus reaction: the rat paw edema test and the rat pleurisy assay. We also applied two methods related with the non-specific acute inflammation: the mouse ear edema test, and the mouse mieloperoxidase activity assay. Results. The results obtained in all tests established a significant anti-inflammatory potential of BS. In the rat paw edema test we found an inhibitory effect which goes from 50-70%; in the rat pleurisy assay our findings with respect to the volume of pleural exuded showed a reduction of 46%, as well as a 20% low amount of neutrophils in comparison with the level of the control group. In the mouse ear edema test we found a mean inflammatory inhibition of 75%, and with respect to mieloproxidase activity the results showed a significant inhibition induced by the three doses of BS. Conclusions. In the present study we determined a potent anti-inflammatory capacity of BS in specific and nonspecific types of acute inflammation in rodents.

show abstract

The Arthus Reaction in Rodents: Species-Specific Requirement of Complement

Cited by 56 publications

References 29 publications

Intracarotid delivery of oncolytic HSV vector G47Δ to metastatic breast cancer in the brain

Intracarotid delivery of oncolytic HSV vector G47Δ to metastatic breast cancer in the brain

Mucosal Clearance of Capsule-Expressing Bacteria Requires Both TLR and Nucleotide-Binding Oligomerization Domain 1 Signaling

Evaluation of the Anti-Inflammatory Capacity of Beta-Sitosterol in Rodent Assays

Contact Info

Product

Resources

About