The Art of Amphibian Science

Smith, Stephen A.; Stoskopf, Michael K.

doi:10.1093/ilar.48.3.179

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Xenopus is an invaluable tool for the study of embryology and vertebrate development, basic cell and molecular biology, genomics, neurobiology and toxicology, as well as for modelling of human diseases (Hellsten et al 2010). This is a model type of frog to use for research purposes, especially in the area of regenerative medicine (Krylov et al 2007(Krylov et al , 2010Smith & Stoskopf 2007). The advantage of the genus Xenopus, unlike other amphibians, is its higher tolerance to environmental fluctuations, ability to feed on dead material, and relatively simple breeding in laboratory conditions.…”

Section: Introductionmentioning

confidence: 99%

Influence of temperature on reproduction and length of metamorphosis in Xenopus laevis (Amphibia: Anura)

Babošová

Vašeková

Porhajašová

et al. 2018

The European Zoological Journal

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Research was conducted on breeding and metamorphosis of a species of the African clawed frog (Xenopus laevis). The experimental group consisted of 10 individuals each of albino and natural forms. Specimens were divided into pairs and numbered for better tracking. In experimental ponds, conditions simulating the mating season were created, which led to 100% reproduction of all the pairs. The aim of the work was to determine the effects of temperature on the gender of resulting metamorphic specimens, and how the length of metamorphosis affects their gender. Specimens were divided into two groups. The first group consisted of specimens bred at 19°C, and the second group of specimens were bred at 24°C. Mating covered the period from 5 May to 25 June 2016. Observations were completed on 31 October 2016. Five hundred and fourteen individual metamorphic specimens were bred during the experiment. The research shows that the length of metamorphosis has an effect on gender, in favour of females. The effect of temperature on gender was not confirmed. The experimental results can be used as a basis for similar research, since this topic has not been discussed previously.

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Section: Introductionmentioning

confidence: 99%

Influence of temperature on reproduction and length of metamorphosis in Xenopus laevis (Amphibia: Anura)

Babošová

Vašeková

Porhajašová

et al. 2018

The European Zoological Journal

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Urbanization and altitude impact on gut microbiome of an Andean frog (Pristimantis unistrigatus)

Carphio

Cisneros-Heredia

Caicedo

et al. 2021

Preprint

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The analysis of the intestinal microbiome in amphibians provides insights of the anthropogenic environmental impact. Pristimantis unistrigatus is an Andean amphibian species whose distribution has been recorded in Ecuador and Colombia, ranging from endemic elfin forests to urban gardens. In this study, we focus on the analysis of the P. unistrigatus microbiome 16S rRNA gene sequencing. A total of 32 specimens of P. unistrigatus were collected and analyzed from 4 locations in the Valley of Quito, Ecuador, characterized by several urban environments and altitudes. The results show that the relative abundance of bacteria is significantly different amongst groups. Clostridiales are proportionally more abundant in rural and lower altitude locations, while Erysipelotrichaceae, Desulfovibrionaceae, Enterobacteriaceae, Bacteroidaceae and Lachnospiraceae are found at higher elevations. These results highlight the importance of the evolution of the microbiome as a tool of adaptation and survival of amphibians in the present-day changing ecosystems undergoing anthropogenic stresses.ImportanceAmphibians constitutes one of the groups most vulnerable to environmental alterations. Due to their sophisticated reproductive and breeding requirements and their permeable skins to breathe, amphibians are compulsively studied as ecological indicators. The destruction of pristine habitats occurred all over the planet in recent decades has caused a catastrophic decline in amphibian populations for many species everywhere. Ecuador, being one of the most biodiverse country on Earth, hosts a huge variety of amphibians, thus offering a unique possibility of studying the biology of the amphibian species living in its ecosystems, and how they adapt to changing habitats. A direct way to diagnose the status of an amphibian population is to study the gut microbiome of the individual specimens. The gut microbiome is closely related to the host’s health and so to its ability to adapt and survive. An important output of this study is to offer indications and tools useful to conservation programs before irreversible damages are caused to the habitats and the amphibians’ populations still thriving in them.

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The Art of Amphibian Science

Cited by 2 publications

References 18 publications

Influence of temperature on reproduction and length of metamorphosis in Xenopus laevis (Amphibia: Anura)

Influence of temperature on reproduction and length of metamorphosis in Xenopus laevis (Amphibia: Anura)

Urbanization and altitude impact on gut microbiome of an Andean frog (Pristimantis unistrigatus)

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