The ART and science of sperm mitophagy

Song, Won Hee; Yi, Young Joo; Sutovsky, Miriam; Meyers, Stuart A.; Šutovský, Peter

doi:10.1080/15548627.2016.1239004

Cited by 14 publications

(8 citation statements)

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“…Of note, an additional group of nuclear proteins have been detected in this study, which, although not being associated to chromatin, is interesting to highlight. This is the case of the proteosomal proteins, since the proteosomal regulation is essential not only for the correct development of spermatogenesis, but also for the early events occurring at fertilization, which includes the process of sperm mitophagy, where potentially damaged paternal mitochondrial DNA is eliminated from the early embryo (Song et al 2016;Sutovsky 2018).…”

Section: Discussionmentioning

confidence: 99%

Identification of a complex population of chromatin-associated proteins in the European sea bass (Dicentrarchus labrax) sperm

Barrachina

Anastasiadi

Jodar

et al. 2018

Systems Biology in Reproductive Medicine

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A very common conception about the function of the spermatozoon is that its unique role is to transmit the paternal genome to the next generation. Most of the sperm genome is known to be condensed in many species by protamines, which are small and extremely positively charged proteins (50-70% arginine) with the functions of streamlining the sperm cell and protecting its DNA. However, more recently, it has been shown in mammals that 2-10% of its mature sperm chromatin is also associated to a complex population of histones and chromatin-associated proteins differentially distributed in the genome. These proteins are transferred to the oocyte upon fertilization and may be involved in the epigenetic marking of the paternal genome. However, little information is so far available on the additional potential sperm chromatin proteins present in other protamine-containing non-mammalian vertebrates detected through high-throughput mass spectrometry. Thus, we started the present work with the goal of characterizing the mature sperm proteome of the European sea bass, with a particular focus on the sperm chromatin, chosen as a representative of non-mammalian vertebrate protamine-containing species. Proteins were isolated by acidic extraction from purified sperm cells and from purified sperm nuclei, digested with trypsin, and subsequently the peptides were separated using liquid chromatography and identified through tandem mass spectrometry. A total of 296 proteins were identified. Of interest, the presence of 94 histones and other chromatin-associated proteins was detected, in addition to the protamines. These results provide phylogenetically strategic information, indicating that the coexistence of histones, additional chromatin proteins, and protamines in sperm is not exclusive of mammals, but is also present in other protamine-containing vertebrates. Thus, it indicates that the epigenetic marking of the sperm chromatin, first demonstrated in mammals, could be more fundamental and conserved than previously thought. Abbreviations: AU-PAGE: acetic acid-urea polyacrylamide gel electrophoresis; CPC: chromosomal passenger complex; DTT: dithiothreitol; EGA: embryonic genome activation; FDR: false discovery rate; GO: Gene Ontology; IAA: iodoacetamide; LC: liquid chromatography; LC-MS/MS: liquid chromatography coupled to tandem mass spectrometry; MS: mass spectrometry; MS/MS: tandem mass spectrometry; MW: molecular weight; PAGE: polyacrylamide gel electrophoresis; PBS: phosphate buffered saline; SDS: sodium dodecyl sulfate; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis; TCA: trichloroacetic acid.

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Section: Discussionmentioning

confidence: 99%

Identification of a complex population of chromatin-associated proteins in the European sea bass (Dicentrarchus labrax) sperm

Barrachina

Anastasiadi

Jodar

et al. 2018

Systems Biology in Reproductive Medicine

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“…For years, the maternal inheritance of the mitochondrial genome was considered as the result of a large dilution of paternal mitochondria contribution with respect to maternal load ( Song et al., 2016b ; Sutovsky and Song, 2018 ). Now we know that it relies on a combination of various active mechanisms that prevent the transmission of sperm mitochondrial genome.…”

Section: Introductionmentioning

confidence: 99%

Mitophagy of polarized sperm-derived mitochondria after fertilization

et al. 2021

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“…Paternal mitochondria removal in zygote is a key step to ensure maternal inheritance of mitochondria. Both ubiquitin proteasome system and sperm mitophagy occurred during the elimination process [46][47][48][49]. Studies in the porcine zygote suggested that a combined action of SQSTM1-dependent autophagy and VCP-mediated ubiquitination of sperm mitochondrial proteins was responsible for sperm mitophagy [47,48].…”

Section: Introductionmentioning

confidence: 99%

“…Both ubiquitin proteasome system and sperm mitophagy occurred during the elimination process [46][47][48][49]. Studies in the porcine zygote suggested that a combined action of SQSTM1-dependent autophagy and VCP-mediated ubiquitination of sperm mitochondrial proteins was responsible for sperm mitophagy [47,48]. In Caenorhabditis elegans, LC3 was a key regulator to control the fate of sperm mitochondria via autophagosome targeted to the pericentrosomal area [50].…”

Section: Introductionmentioning

confidence: 99%

SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy

Zhang

Xu²,

Hu³

et al. 2020

Cell Death Differ

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Selective autophagic degradation of mitochondria (mitophagy) is important in maintaining proper cellular homeostasis. Here, we found that SPATA33 is a novel autophagy mediator for mitophagy in testis. The SPATA33 protein localizes on mitochondria via its binding of the carboxyl terminal with the outer mitochondrial membrane protein VDAC2. Upon starvation induction, SPATA33 is recruited to autophagosome by binding the autophagy machinery ATG16L1 via its N-terminal along with mitochondria. Notably, Spata33 knockout inhibited autophagy and overexpression can promote autophagosome formation for mitochondrial sequestration. Therefore, SPATA33 confers selectivity for mitochondrial degradation and promotes mitophagy in male germline cells.

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The ART and science of sperm mitophagy

Cited by 14 publications

References 0 publications

Identification of a complex population of chromatin-associated proteins in the European sea bass (Dicentrarchus labrax) sperm

Identification of a complex population of chromatin-associated proteins in the European sea bass (Dicentrarchus labrax) sperm

Mitophagy of polarized sperm-derived mitochondria after fertilization

SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy

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