The arsenic-phytoremediation potential of genetically modified pseudomonas spp

Сизова, О. И.; Кочетков, В. В.; Boronin, A. M.

doi:10.1007/1-4020-4688-x_14

Cited by 3 publications

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“…The use of phytoremediation to restore contaminated soils of abandoned metal-mine working and to reduce the impact of polychlorinated biphenyls (PCBs) from dumps and mitigation of contamination in on-going coal mine discharges has been reported (Li, 2005;Azevedo et al, 2005;Sizova et al, 2006). The efficiency and time to effect clean up by phytoremediation is a function of the plant type and population on contaminated site, concentration of pollutants and extent of pollution, soil class and prevailing soil condition which varies with locations.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the phytoremediation potential of Panicum maximum (guinea grass) for selected heavy metal removal from contaminated soils

Olatunji¹,

Ximba²,

Fatoki³

et al. 2014

Afr. J. Biotechnol.

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Non-vascular plants have potential for rapid uptake of metals, but are rarely used for phytoremediation because of their short life cycle. This property can however be advantageously used in a number of metal removal cycles within a short time. The selection of promising plants is critical to success of phytoremediation. The potential for heavy metal uptake by Panicum maximum a non-vascular plant was investigated using pot plant experiments. Seventy-two (72) pots of 7 L capacity were each filled with 5 kg of mixed soil collected from the Fadama (fertile soil) and College of Animal Sciences and Livestock Production farms (less fertile) of the University of Agriculture Abeokuta, Ogun State Nigeria. The pots were divided into six groups of twelve pots each. Each of the group of 12 pots was further divided into three groups of four pots each, in which P. maximum were planted. Five of the six groups were treated with 20, 50, 75, 100 and 120 ppm each of Pb 2+ , Cr 3+ and Cd 2+ and the sixth served as control. Levels of Pb 2+ decreased from 1.40 to 1.05 µg/g and 1.57 to 1.30 µg/g in soils treated with 20 and 120 ppm of Pb 2+ , respectively. Generally there was increase in Pb 2+ uptake by the different tissues of P. maximum ranging between 0.21 to 0.38 µg/g, 0.18 to 0.30 µg/g, and 0.09 to 0.18 µg/g in root, stem and foliage, respectively. Corresponding bioaccumulation factors (BAF) ranged between 0.21 to 0.45, 0.17 to 0.35 and 0.08 to 0.21. Metal uptake followed similar trends for Cr 3+ and Cd 2+ in plant tissues. The BAF values for Cr 3+ ranged between 0.31 to 0.69 in root, 0.17 to 0.52 in stem and 0.07 to 0.34 in foliage. Similar values for Cd 2+ were 0.14 to 0.53, 0.10 to 0.44 and 0.05 to 0.37. Accumulation of heavy metals in P. maximum ranged from 13 to 45%, Pb 2+ ; 13 to 65%, Cr 3+ and 11 to 52%, Cd 2+ of the soil concentration level with tissue abundance decreasing in the order Cr 3+ > Pb 2+ > Cd 2+ . Furthermore, the concentration of metals in P. maximum tissues decreased in the order root > stem > foliage. The phytoremediation of Pb 2+ , Cr 3+ and Cd 2+ contaminated soils with P. maximum seems to be promising under the conditions of the experiment. Obvious signs of phyto-toxicity however appeared in plants exposed to 120 ppm Pb 2+ and Cd 2+ at day twenty-three, suggesting that P. maximum may be a moderate metal accumulator.

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Section: Introductionmentioning

confidence: 99%

Assessment of the phytoremediation potential of Panicum maximum (guinea grass) for selected heavy metal removal from contaminated soils

Olatunji¹,

Ximba²,

Fatoki³

et al. 2014

Afr. J. Biotechnol.

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“…The results indicate that the As(V) reductase activity was associated with cytosolic fractions and not with membrane fractions. A cytoplasmic arsenate reductase catalyzes reduction of the less toxic arsenate to more toxic arsenite and is the most well-known mechanism of arsenic resistance, described in many different bacteria [46,47]. The As(V) reduction by the cell-free extracts as a function of time is shown in Fig.…”

Section: Effect Of Selenate On Arsenate Reduction By Strain Drbs1mentioning

confidence: 99%

Pentavalent Arsenate Reductase Activity in Cytosolic Fractions of Pseudomonas sp., Isolated from Arsenic-Contaminated Sites of Tezpur, Assam

Srivastava

Madamwar

Subramanian

2009

Appl Biochem Biotechnol

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Pentavalent arsenate reductase activity was localized and characterized in vitro in the cytosolic fraction of a newly isolated bacterial strain from arsenic-contaminated sites. The bacterium was gram negative, rod-shaped, nonmotile, non-spore-forming, and noncapsulated, and the strain was identified as Pseudomonas sp. DRBS1 following biochemical and molecular approaches. The strain Pseudomonas sp. DRBS1 exhibited enzymatic machinery for reduction of arsenate(V) to arsenite(III). The suspended culture of the bacterium reduced more than 97% of As(V) (40-100 mM) to As(III) in 48 h. The growth rate and total cellular yield decreased in the presence of higher concentration of arsenate. The suspended culture repeatedly reduced 10 mM As(V) within 5 h up to five consecutive inputs. The cell-free extracts reduced 86% of 100 microM As(V) in 40 min. The specific activity of arsenate reductase enzyme in the presence of 100 microM arsenate is 6.68 micromol/min per milligram protein. The arsenate reductase activity is maximum at 30 degrees C and at pH 5.2. The arsenate reductase activity increased in the presence of electron donors like citrate, glucose, and galactose and metal ions like Cd(+2), Cu(+2), Ca(+2), and Fe(+2). Selenate as an electron donor also supports the growth of strain DRBS1 and significantly increased the arsenate reduction.

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“…Furthermore, biomass of canola was highest in transgenic canola inoculated with the PGPR Enterobacter cloacae CAL2 thus having highest arsenate accumulated. Sizova et al (2006) reported the effect of inoculation of genetically modified Pseudomonas aureofaciens on the survival and As accumulation of sorghum. Three genes for arsenic tolerance, arsR (the transcription regulator), arsB (encoding a membrane protein) and arsC (encoding arsenate reductase) and gltA (citrate synthase, the gene for phosphate solubilization) were inserted to P. aureofaciens.…”

Section: Bacteria-assisted Phytoremediationmentioning

confidence: 99%

Exploring the Potential of Bacteria-Assisted Phytoremediation of Arsenic-Contaminated Soils

Shagol¹,

Chauhan²,

Kim³

et al. 2011

Korean Journal of Soil Science and Fertilizer

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Arsenic pollution is a serious global concern which affects all life forms. Being a toxic metalloid, the continued search for appropriate technologies for its remediation is needed. Phytoremediation, the use of green plants, is not only a low cost but also an environmentally friendly approach for metal uptake and stabilization. However, its application is limited by slow plant growth which is further aggravated by the phytotoxic effect of the pollutant. Attempts to address these constraints were done by exploiting plant-microbe interactions which offers more advantages for phytoremediation. Several bacterial mechanisms that can increase the efficiency of phytoremediation of As are nitrogen fixation, phosphate solubilization, siderophore production, ACC deaminase activity and growth regulator production. Many have been reported for other metals, but few for arsenic. This mini-review attempts to present what has been done so far in exploring plants and their rhizosphere microbiota and some genetic manipulations to increase the efficiency of arsenic soil phytoremediation.

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The arsenic-phytoremediation potential of genetically modified pseudomonas spp

Cited by 3 publications

References 0 publications

Assessment of the phytoremediation potential of Panicum maximum (guinea grass) for selected heavy metal removal from contaminated soils

Assessment of the phytoremediation potential of Panicum maximum (guinea grass) for selected heavy metal removal from contaminated soils

Pentavalent Arsenate Reductase Activity in Cytosolic Fractions of Pseudomonas sp., Isolated from Arsenic-Contaminated Sites of Tezpur, Assam

Exploring the Potential of Bacteria-Assisted Phytoremediation of Arsenic-Contaminated Soils

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