The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research*

Abstract

Reproducible science requires transparent reporting. The ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments) were originally developed in 2010 to improve the reporting of animal research. They consist of a checklist of information to include in publications describing in vivo experiments to enable others to scrutinise the work adequately, evaluate its methodological rigour, and reproduce the methods and results. Despite considerable levels of endorsement by funders and journals over the years, adherence to the guidelines has been inconsistent, and the anticipated improvements in the quality of reporting in animal research publications have not been achieved. Here, we introduce ARRIVE 2.0. The guidelines have been updated and information reorganised to facilitate their use in practice. We used a Delphi exercise to prioritise and divide the items of the guidelines into 2 sets, the “ARRIVE Essential 10,” which constitutes the minimum requirement, and the “Recommended Set,” which describes the research context. This division facilitates improved reporting of animal research by supporting a stepwise approach to implementation. This helps journal editors and reviewers verify that the most important items are being reported in manuscripts. We have also developed the accompanying Explanation and Elaboration document, which serves (1) to explain the rationale behind each item in the guidelines, (2) to clarify key concepts, and (3) to provide illustrative examples. We aim, through these changes, to help ensure that researchers, reviewers, and journal editors are better equipped to improve the rigour and transparency of the scientific process and thus reproducibility.

Integration of multi-omics approaches for functional characterization of muscle related selective sweep genes in Nanchukmacdon

“…Samples were taken from healthy Male pigs belong to the same farm in Jeju Island with an average age of 2 years for (N = 1–6), and 3 years for (N = 7–10) (Additional_Table 4 ). All the experimental procedures were verified and approved by the National Institute of Animal Science, and carried out in compliance with the ARRIVE guidelines 51 . Whole-genome re-sequencing was performed from the blood sample taken from post-harvest Nanchukmacdon (N = 10), RNAseq data was generated for Nanchukmacdon (N = 5) pair-end data after isolation of muscle tissue using TRIzol method following the manufacturer guideline and reported earlier 15 .…”

The ‘R’ principles in laboratory animal experiments

“…The PREPARE (Planning Research and Experimental Procedures on Animals: Recommendations for Excellence) checklist suggests that the quality of scientific result is dependent upon planning and conducting [ 7 ]. In addition, transparent reporting of research findings is essential for the reproducibility of animal study, resulting in the realization of the scientific benefits to society as the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines suggest [ 8 ]. While adhering to ARRIVE recommended items, ethical review and realization of the Three Rs principles can be evaluated [ 9 ].…”

Effect of relative social rank within a social hierarchy on neural activation in response to familiar or unfamiliar social signals

“…All experiments were approved by the Columbia University Institutional Animal Care and Use Committee (IACUC Protocol No. AC-AAAP5405) and were conducted in accordance with the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines 76 , National Health Institute (NIH; Bethesda, MD, USA) and Columbia University IACUC animal care guidelines.…”