The architecture of Cidec-mediated interfaces between lipid droplets

Ganeva, Iva; Lim, Koini; Boulanger, Jérôme; Hoffmann, Patrick C.; Muriel, Olivia; Borgeaud, Alicia C.; Hagen, Wim J. H.; Savage, David B.; Kukulski, Wanda

doi:10.1016/j.celrep.2023.112107

Cited by 9 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We summarize the recent progress in LD fusion and growth mediated by CIDE proteins and their regulators. The enrichment and condensation of CIDE proteins at LDCSs are necessary for LD fusion to form lipid-permeable passageways [102,113]. Besides, different regulatory factors and their specific distribution contribute to the function of CIDE proteins in LD fusion and LD dynamics.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

CIDE proteins and their regulatory mechanisms in lipid droplet fusion and growth

Xu,

Li,

et al. 2024

FEBS Letters

View full text Add to dashboard Cite

The cell death‐inducing DFF45‐like effector (CIDE) proteins, including Cidea, Cideb, and Cidec/Fsp27, regulate various aspects of lipid homeostasis, including lipid storage, lipolysis, and lipid secretion. This review focuses on the physiological roles of CIDE proteins based on studies on knockout mouse models and human patients bearing CIDE mutations. The primary cellular function of CIDE proteins is to localize to lipid droplets (LDs) and to control LD fusion and growth across different cell types. We propose a four‐step process of LD fusion, characterized by (a) the recruitment of CIDE proteins to the LD surface and CIDE movement, (b) the enrichment and condensate formation of CIDE proteins to form LD fusion plates at LD–LD contact sites, (c) lipid transfer through lipid‐permeable passageways within the fusion plates, and (d) the completion of LD fusion. Lastly, we outline CIDE‐interacting proteins as regulatory factors, as well as their contribution in LD fusion.

show abstract

Section: Discussionmentioning

confidence: 99%

“…2B). It should be noted that compared with no tag CIDE, GFP-tagged CIDE proteins exhibit reduced activity in LD fusion [39,113].…”

Section: Key Regulatory Factors In Cidemediated Ld Fusion and Growthmentioning

confidence: 99%

CIDE proteins and their regulatory mechanisms in lipid droplet fusion and growth

Xu,

Li,

et al. 2024

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…multiple cells); XY resolution of 10-20 nm; Z resolution dictated by the thickness of the sectioned area (~30 nm) RT Focused Ion Beam SEM [32] Typically used for volumes of one cell, isotropic pixel size XYZ with a maximum resolution of ~5 9 5 9 5 nm Cryo-EM: optimal structural preservation of hydrated samples, close to native state. Fine details, such as LD core structural features and molecular tethers, can be directly observed In situ cryo-ET [46,47,76] High resolution in XY in the nm range; Currently low throughput due to non-routine multistep sample preparation Cryo-FIB-SEM [40,151,152] Imaging still requires improvement due to charging-related artifacts and a low signal-to-noise ratio; 3D reconstruction of large volumes (whole single or multiple cells) is possible…”

Section: Em Methods Notesmentioning

confidence: 99%

“…Cryo‐ET showed that at these contact sites, the apposed LD monolayers remain separate, maintaining an approximate 10 nm distance from each other. Importantly, large LDs were often indented by smaller LDs, directly informing on their respective relative internal pressures, the difference of which drives the lipid transfer process [47,126]. This exemplifies how EM can help bridge the gap between the biophysical, structural, and cell biology perspectives on LDs.…”

Section: Characterization Of Lds At the Organelle And Sub‐organelle L...mentioning

confidence: 99%

Zooming into lipid droplet biology through the lens of electron microscopy

Dudka,

Salo,

Mahamid

2024

FEBS Letters

View full text Add to dashboard Cite

Electron microscopy (EM), in its various flavors, has significantly contributed to our understanding of lipid droplets (LD) as central organelles in cellular metabolism. For example, EM has illuminated that LDs, in contrast to all other cellular organelles, are uniquely enclosed by a single phospholipid monolayer, revealed the architecture of LD contact sites with different organelles, and provided near‐atomic resolution maps of key enzymes that regulate neutral lipid biosynthesis and LD biogenesis. In this review, we first provide a brief history of pivotal findings in LD biology unveiled through the lens of an electron microscope. We describe the main EM techniques used in the context of LD research and discuss their current capabilities and limitations, thereby providing a foundation for utilizing suitable EM methodology to address LD‐related questions with sufficient level of structural preservation, detail, and resolution. Finally, we highlight examples where EM has recently been and is expected to be instrumental in expanding the frontiers of LD biology.

show abstract

“…Enriched in white adipocytes where it is located at contact sites between LDs [22]. Facilitates directional neutral lipid transfer from the smaller to the larger droplet [22]. Required for the formation of large unilocular LDs in white adipocytes [23].…”

Section: Gene/protein Name and Disease Inheritance Pattern Disease Pu...mentioning

confidence: 99%

The role of lipid droplet associated proteins in inherited human disorders

Duan,

Savage

2023

FEBS Letters

Self Cite

View full text Add to dashboard Cite

The architecture of Cidec-mediated interfaces between lipid droplets

Cited by 9 publications

References 35 publications

CIDE proteins and their regulatory mechanisms in lipid droplet fusion and growth

CIDE proteins and their regulatory mechanisms in lipid droplet fusion and growth

Zooming into lipid droplet biology through the lens of electron microscopy

The role of lipid droplet associated proteins in inherited human disorders

Contact Info

Product

Resources

About