The Archaellum of<i>Methanospirillum hungatei</i>is Electrically Conductive

Walker, David M.; Martz, Eric; Holmes, Dawn E.; Zhou, Zimu; Nonnenmann, Stephen S.; Lovley, Derek R.

doi:10.1101/458356

Cited by 20 publications

(34 citation statements)

References 22 publications

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“…The e-PNs were conductive with an ohmic-like current-voltage response (Figure 4d). The conductance of the individual e-PNs, 4.3 + 0.8 nS (n=9), compared well with the previously reported 15 conductance of 4.5 + 0.3 nS for individual e-PNs expressed in G. sulfurreducens .…”

Section: Resultssupporting

confidence: 87%

“…The conductance of individual e-PNs was evaluated on highly oriented pyrolytic graphite with atomic force microscopy employing a conductive tip, as previously described 15 . The diameter of the e-PNs was 3 + 0.04 nm (n=18; 6 measurements on 3 independent pili) the same as e-PNs expressed with G. sulfurreducens (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

“…Benefits include much lower cost and greater flexibility in e-PN design options with a production platform fueled with inexpensive, renewable feedstocks. A diversity of bacteria and archaea assemble peptides that show homology to bacterial type IV pilins into e-PNs 11-15 , but the e-PNs of Geobacter sulfurreducens have been most intensively investigated 1, 16 . G. sulfurreducens e-PNs can be fabricated in vivo with acetate as the carbon and energy source.…”

Section: Introductionmentioning

confidence: 99%

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An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires

Ueki

Walker

Woodard

et al. 2019

Preprint

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11Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs) are a 12 revolutionary electronic material. They offer novel options for electronic sensing applications 13 and have the remarkable ability to harvest electrical energy from atmospheric humidity. 14 However, technical constraints limit mass cultivation and genetic manipulation of G. 15 sulfurreducens. Therefore, we designed a strain of Escherichia coli to express e-PNs by 16 introducing a plasmid that contained an inducible operon with E. coli genes for type IV pili 17 biogenesis machinery and a synthetic gene designed to yield a peptide monomer that could be 18 assembled into e-PNs. The e-PNs expressed in E. coli, and harvested with a simple filtration 19 method, had the same diameter (3 nm) and conductance as e-PNs expressed in G. 20 sulfurreducens. These results, coupled with the robustness of E. coli for mass cultivation and the 21 extensive E. coli toolbox for genetic manipulation, greatly expands opportunities for large-scale 22 fabrication of novel e-PNs. 23 24

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires

Ueki

Walker

Woodard

et al. 2019

Preprint

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“…Recently, the phylogenetic and structural diversity of e-pili has expanded beyond Geobacter spp. with the discovery of strongly conductive pili in clades outside of Geobacter genera, including Syntrophus aciditrophicus ( Deltaproteobacteria/Syntrophobacterales ), Desulfurivibrio alkaliphilus ( Deltaproteobacteria/Desulfobacterales ), Calditerrivibrio nitroreducens ( Deferribacteres ), and the archaeon Methanospirillum hungatei ( Euryarchaeota/Methanomicrobiales ) (Walker et al, 2018; Walker et al, 2019a; Walker et al, 2019b) (Table 1) . Pilin genes in these four microbes are much longer (110-182 aa) than in Geobacter spp., but have similar aromaticity (11-13%) and similar maximum aromatic-free gaps (22-35 aa).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes

Bray

Padilla

et al. 2019

Preprint

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Summary Electroactive type IV pili, or e‐pili, are used by some microbial species for extracellular electron transfer. Recent studies suggest that e‐pili may be more phylogenetically and structurally diverse than previously assumed. Here, we used updated aromatic density thresholds (≥9.8% aromatic amino acids, ≤22‐aa aromatic gaps and aromatic amino acids at residues 1, 24, 27, 50 and/or 51, and 32 and/or 57) to search for putative e‐pilin genes in metagenomes from diverse ecosystems with active microbial metal cycling. Environmental putative e‐pilins were diverse in length and phylogeny, and included truncated e‐pilins in Geobacter spp., as well as longer putative e‐pilins in Fe(II)‐oxidizing Betaproteobacteria and Zetaproteobacteria.

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“…as their properties can readily be tuned with genetic modifications to the pilin monomer; they can be incorporated into polymers to produce composite electronic materials; and they have dynamic sensing properties 3,4,11 . Other bacteria produce e-pili from pilin monomers 12,13 and the archaeon Methanospirillum hungatei expresses an electrically conductive protein filament from archaellin monomers 14 . Both e-pili and multi-heme outer-surface cytochromes have been implicated as being important for Geobacter`s long-range extracellular electron exchange with other cells and soil minerals 15,16 .…”

mentioning

confidence: 99%

Structure of a cytochrome-based bacterial nanowire

Filman

Marino

Ward

et al. 2018

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Electrically conductive pili from Geobacter species, termed bacterial "nanowires", are intensely studied for their biological significance and potential in the development of new materials. We have characterized a unique nanowire from conductive G. sulfurreducens pili preparations by cryo-electron microscopy composed solely of the c-type cytochrome OmcS. We present here, at 3.4 Å resolution, a novel structure of a cytochrome-based filament and discuss its possible role in long-range biological electron transport. Summary sentence:Cryo-electron microscopy reveals the remarkable assembly of a c-type cytochrome into filaments comprising a heme-based bacterial nanowire. Main textMicrobially produced protein nanowires are of profound interest due to their proposed role in global carbon and metal cycling and promise for myriad practical applications 1-4 . Among the most thoroughly characterized are the electrically conductive pili (e-pili) of Geobacter species, which are comprised of pilin protein monomers [5][6][7] . Geobacter e-pili enable longrange (µms) extracellular electron transfer to Fe(III) minerals and other microbial species, which is important in the biogeochemistry of diverse anaerobic soils and sediments 3,8 . Geobacter species are among the most effective microbes in converting organic compounds into electricity because they can form thick electrically conductive e-pili biofilms 9,10 . e-Pili purified from cells show potential as sustainably produced protein nanowires for electronics,

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The Archaellum ofMethanospirillum hungateiis Electrically Conductive

Cited by 20 publications

References 22 publications

An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires

An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires

Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes

Structure of a cytochrome-based bacterial nanowire

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