The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction resolvase Hjc define alternative pathways that are essential for cell viability in Haloferax volcanii

Lestini, Roxane; Duan, Zengqiang; Allers, Thorsten

doi:10.1016/j.dnarep.2010.06.012

Cited by 57 publications

(91 citation statements)

References 43 publications

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“…During the preparation of this manuscript, a report was published describing genetic analyses of H. volcanii deletion mutant strains for the hef and hjc genes (Lestini et al, 2010). Their results indicated that Hef is essential for cell viability in the absence of Hjc.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis

Fujikane

Ishino

et al. 2010

Genes Genet. Syst.

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Extensive biochemical and structural analyses have been performed on the putative DNA repair proteins of hyperthermophilic archaea, in contrast to the few genetic analyses of the genes encoding these proteins. Accordingly, little is known about the repair pathways used by archaeal cells at high temperature. Here, we attempted to disrupt the genes encoding the potential repair proteins in the genome of the hyperthermophilic archaeon Thermococcus kodakaraensis. We succeeded in isolating null mutants of the hjc, hef, hjm, xpb, and xpd genes, but not the radA, rad50, mre11, herA, nurA, and xpg/fen1 genes. Phenotypic analyses of the gene-disrupted strains showed that the xpb and xpd null mutants are only slightly sensitive to ultraviolet (UV) irradiation, methyl methanesulfonate (MMS) and mitomycin C (MMC), as compared with the wild-type strain. The hjm null mutant showed sensitivity specifically to mitomycin C. On the other hand, the null mutants of the hjc gene lacked increasing sensitivity to any type of DNA damage. The Hef protein is particularly important for maintaining genome homeostasis, by functioning in the repair of a wide variety of DNA damage in T. kodakaraensis cells. Deletion of the entire hef gene or of the segments encoding either its nuclease or helicase domain produced similar phenotypes. The high sensitivity of the Δhef mutants to MMC suggests that Hef performs a critical function in the repair process of DNA interstrand crosslinks. These damage-sensitivity profiles suggest that the archaeal DNA repair system has processes depending on repair-related proteins different from those of eukaryotic and bacterial DNA repair systems using homologous repair proteins analyzed here.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis

Fujikane

Ishino

et al. 2010

Genes Genet. Syst.

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“…In this regard, it is worth noting that PabNucS was found to be associated with Hef nuclease in pulldown experiments (9). Recent genetic analyses have shown that Hef is involved in numerous DNA repair pathways in the archaeal cells, including resolution of stalled replication forks, interstrand cross-links, and NER (61,62). Up to now, there is no evidence of direct interaction between Hef and PCNA, however, crenarchaeal XPF, the shorter form of Hef lacking the helicase segment, was shown to be dependent on the PCNA for its nuclease activity, activating the catalytic step by 4 orders of magnitude (22,63,64).…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Pyrococcus abyssi NucS Endonuclease Activity by Replication Clamp at Functional and Structural Levels

Crézé¹,

Ligabue²,

Laurent³

et al. 2012

Journal of Biological Chemistry

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Background: NucS, new bipolar nuclease acting on branched DNA repair substrates, interacts with proliferating cell nuclear antigen (PCNA). Results: PCNA and NucS form a stable 1:1 complex and PCNA directs the activity of NucS toward single-stranded/doublestranded DNA junctions in branched DNA substrates. Conclusion: PCNA regulates NucS activity, preventing the nonspecific cleavage of NucS on the chromatin. Significance: This study will help understand how PCNA regulates its client enzymes.

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“…For example, the UvrABC complex, whose origin is bacterial, is in haloarchaeas such as Hfx. volcanii, where it is fully active (Lestini, Duan & Allers, 2010). The…”

Section: Introductionmentioning

confidence: 99%

Anaerobic Metabolism in Haloferax Genus

Torregrosa-Crespo

Martínez‐Espinosa

Esclapez

et al. 2016

Advances in Microbial Physiology

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A number of species of Haloferax genus (halophilic Archaea) are able to grow micro aerobically or even anaerobically using different alternative electron acceptors such as fumarate, nitrate, chlorate, dimethyl sulphoxide, sulphide and/or trimethylamine. This metabolic capability is also shown by other species of the Halobacteriaceae and Haloferacaceae families (Archaea domain) and it has been mainly tested by physiological studies where cells growth is observed under anaerobic conditions in the presence of the mentioned compounds. This work summarises the main reported features on anaerobic metabolism in the Haloferax, one of the better described haloarchaeal genus with significant potential uses in Biotechnology and Bioremediation. Special attention has been paid to denitrification, also called nitrate respiration. This pathway has been studied so far from KEY WORDSHaloarchaea, denitrification, anaerobic metabolism, Nox emissions, bioremediation.

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The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction resolvase Hjc define alternative pathways that are essential for cell viability in Haloferax volcanii

Cited by 57 publications

References 43 publications

Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis

Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis

Modulation of the Pyrococcus abyssi NucS Endonuclease Activity by Replication Clamp at Functional and Structural Levels

Anaerobic Metabolism in Haloferax Genus

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