The Arabs in Africa

Maidhof, Callie

doi:10.2307/j.ctt183p77h.26

Cited by 1 publication

(3 citation statements)

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“…Forward-reverse flow was applied (0.1 mm/s flow velocity, reversal of flow direction every 3 min) over a 2 h seeding period. As previously demonstrated, this procedure yields constructs that are homogeneously seeded with viable cells and channels that remain unblocked and can be perfused with culture medium (Maidhof et al, 2010).…”

Section: 4 Cell Seeding and Culturementioning

confidence: 86%

“…For cell seeding, perfusion loops consisting of cartridges and tubing were assembled as in our previous studies (Maidhof et al, 2010). Briefly, two channelled scaffolds were stacked on top of each other in perfusion cartridges and held in place on the edge by gaskets made from silicone tubing.…”

Section: 4 Cell Seeding and Culturementioning

confidence: 99%

“…In the heart, the blood supply flows through a dense capillary network that minimizes transport distances but also protects myocytes from shear. To recapitulate this situation in vitro, we have pioneered the use of scaffolds with a parallel array of smalldiameter (250 mm) channels (Radisic et al, 2005(Radisic et al, , 2006a(Radisic et al, , 2006b) and developed techniques to uniformly seed these constructs homogeneously with a cardiac cell population without the use of a gel carrier that would block the channels (Maidhof et al, 2010). Perfusion of culture medium through scaffold channels decreased the distances for diffusional transport while protecting the cells from hydrodynamic shear, by mechanisms similar to those utilized by the capillary flow in the heart.…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic perfusion and electrical stimulation applied in concert improved the assembly of engineered cardiac tissue

Maidhof

Tandon

Lee

et al. 2011

J Tissue Eng Regen Med

122

140

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Maintenance of normal myocardial function depends intimately on synchronous tissue contraction driven by electrical activation and on adequate nutrient perfusion in support thereof. Bioreactors have been used to mimic aspects of these factors in vitro to engineer cardiac tissue, but due to design limitations, previous bioreactor systems have yet to simultaneously support nutrient perfusion, electrical stimulation, and unconstrained (i.e., not isometric) tissue contraction. To the best of our knowledge, the bioreactor system described herein is the first to integrate in concert these three key factors. We present the design of our bioreactor and characterize its capability in integrated experimental and mathematical modeling studies. We then culture cardiac cells obtained from neonatal rats in porous, channeled elastomer scaffolds with the simultaneous application of perfusion and electrical stimulation, with controls excluding either one or both of these two conditions. After eight days of culture, constructs grown with the simultaneous perfusion and electrical stimulation exhibited substantially improved functional properties, as evidenced by a significant increase in contraction amplitude (0.23±0.10% vs. 0.14±0.05, 0.13±0.08, or 0.09±0.02% in control constructs grown without stimulation, without perfusion, or either stimulation or perfusion, respectively). Consistently, these constructs had significantly improved DNA contents, cell distribution throughout the scaffold thickness, cardiac protein expression, cell morphology and overall tissue organization than either control group. Thus, the simultaneous application of medium perfusion and electrical conditioning enabled by the use of the novel bioreactor system may accelerate the generation of fully functional, clinically sized cardiac tissue constructs.

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Section: 4 Cell Seeding and Culturementioning

confidence: 86%

Section: 4 Cell Seeding and Culturementioning

confidence: 99%