The Arabidopsis TTL3 protein interconnects brassinosteroid signalling and cytoskeleton during lateral root development

Abstract

Lateral roots are essential components of the plant edaphic interface, contributing to water and nutrient uptake, biotic and abiotic interactions, stress survival, and plant anchorage. We have identified the TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE 3 (TTL3) being related to lateral root emergence and later development. TTL3 interacts with microtubules and potentially interconnects cytoskeletal function with the brassinosteroid signalling pathway. Loss of function of TTL3 leads to a reduced number of emerged lateral roots due to delayed development of lateral root primordia. Lateral root growth of the ttl3 mutant is less sensitive to BR treatment. Timing and spatial distribution of TTL3 expression is consistent with its role in development of lateral root primordia before their emergence and subsequent development into lateral roots. TTL3 is a novel component of the root system morphogenesis regulatory network.

Transcriptome analysis reveals key regulatory genes for root growth related to potassium utilization efficiency in rapeseed (Brassica napus L.)

“…A strong association was found between B naA05G0032700ZS and four genes, BnaC06G0226000ZS, BnaC02G0500800ZS , BnaC01G0183000ZS, and BnaA09G0485400ZS in the green-yellow module ( Figure S4A ). A homolog of BnaA05G0032700ZS identified as tetratricopeptide-repeat thioredoxin-like 3 (TTL3) interfaces with microtubules and may be a link between cytoskeletal function and the brassinosteroid signaling pathway in the formation of lateral roots ( Xin et al., 2020 ).…”