The Arabidopsis transcription factors AtPHL1 and AtHB23 act together promoting carbohydrate transport from pedicel-silique nodes to seeds

Spies, Fiorella Paola; Raineri, Jesica; Miguel, Virginia; Cho, Yuhan; Hong, Jong Chan; Chan, Raquel L.

doi:10.1016/j.plantsci.2021.111133

Cited by 4 publications

(15 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such was the case of AtHB23, used as bait to screen an Arabidopsis TF library, allowing identifying four putative partners. In previous work, we corroborated the interaction between AtHB23 and AtPHR1-like1 (thereafter, AtPHL1) by carrying out independent Y2H and BiFC (bimolecular fluorescence complementation) assays (Spies et al ., 2022). Moreover, the functional meaning of such an interaction was studied in conductive tissues and siliques (Spies et al ., 2022), whereas that with AtMYB68 (At5g65890) remained unexplored.…”

Section: Resultssupporting

confidence: 70%

“…Arabidopsis mutant lines phl1 -1 and −2 (SAIL_731_B09 and (SALK_079505.20.10) and DR5:GFP transgenic plants were obtained from the Arabidopsis Biological Resource Center (ABRC) stock. AtHB23 -silenced plants ( amiR23 ), prAtPHL1:GUS, prAtHB23 L :GUS, and prAtHB23 S :GUS were previously described (Perotti et al ., 2019; Spies et al ., 2022; Perotti et al ., 2022).…”

Section: Methodsmentioning

confidence: 99%

“…A truncated version of AtHB23 (AtHB23ΔAHA, Capella et al ., 2014) was used as bait for yeast two-hybrid screening as previously described (Spies et al ., 2022).…”

Section: Methodsmentioning

confidence: 99%

“…However, the deletion of the AHA motif to avoid transactivation in yeast-two-hybrid (Y2H) assays indicated that the proteins still interact with others. Such was the case of AtHB23 (AtHB23ΔAHA), used as bait to screen an Arabidopsis TF library, which revealed the interaction with four different proteins (Spies et al ., 2022). They were three belonging to the large MYB family, AtMYB68 (At5g65890), AtPHL1 (At5g29000), and AtMYB12 (At2g47460), and AtWRKY43 (At2g46130; Spies et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

“…It was previously reported that AtPHL1 and AtHB23 are coexpressed in the pedicel-silique nodes and the funiculus, interacting to promote sucrose transport (Spies et al ., 2022). In this work, we investigated the interplay between AtHB23, AtMYB68, and AtPHL1 in root development.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

Spies

Perotti

Cho

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Adaptation to soil is a well-regulated process vital for plant life. AtHB23 is a homeodomain-leucine zipper I transcription factor (TF), previously revealed as crucial for plant survival in front of salinity conditions. We wondered whether this TF has partners to achieve this essential function. A TF cDNA library screening, Y2H, BiFC, and CoIP assays were complemented with expression analyses and phenotypic characterizations of silenced, mutant, overexpressor, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and salinity response. The encoding genes coexpress in specific root tissues and developmental stages. In normal conditions, amiR68 silenced plants have less initiated roots, the opposite phenotype to that showed by amiR23 ones. AtMYB68 and AtPHL1 play contrary roles in lateral root elongation. Under salinity, where AtHB23 plays a crucial positive function, AtMYB68 cooperates with it, whereas AtPHL1 obstructs its action impacting survival ability and supporting the complex interaction between AtHB23, AtPHL1, and AtMYB68 in the primary and lateral roots. The root adaptation capability was associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival ability in salinity conditions.

show abstract

Section: Resultssupporting

confidence: 70%

Section: Methodsmentioning

confidence: 99%

“…A truncated version of AtHB23 (AtHB23ΔAHA, Capella et al ., 2014) was used as bait for yeast two-hybrid screening as previously described (Spies et al ., 2022).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

Spies

Perotti

Cho

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

RSL4 regulatory network acts as an early driver of root hair growth at low temperature inArabidopsis thaliana

Lehuedé,

Gabarain,

Moyano

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

The root hair (RH) cells can elongate to several hundred times their initial size, and are an ideal model system for investigating cell size control. Their development is influenced by both endogenous and external signals, which are combined to form an integrative response. Surprisingly, a low temperature condition of 10C causes an increased RH growth in Arabidopsis and in several monocots, even when the development of the rest of the root and aerial parts of the plant are halted. Previously, we demonstrated a strong correlation between the growth response and a significant decrease in nutrient availability in the medium under low temperature conditions. However, the molecular basis responsible for receiving and transmitting signals related to the availability of nutrients in the soil, and their relation to plant development, remain largely unknown. We decided to further investigate the intricate molecular processes behind the particular responsiveness of this root cell type at low temperature. In this study, we have discovered a gene regulatory network (GRN) controlling early transcriptome responses to low temperature. This GNR is commanded by specific transcription factors (FTs), namely ROOT HAIR DEFECTIVE 6-LIKE 4 (RSL4), a member of the homeodomain leucine zipper (HD-Zip I) group I 13 (AtHB13), the trihelix TF GT2-LIKE1 (GTL1), and a previously unidentified MYB-like TF (AT2G01060). Furthermore, we have identified four downstream RSL4 targets AtHB16, AtHB23, EARLY-RESPONSIVE TO DEHYDRATION 7 (ERD7) and ERD10 suggesting their participation in the regulation of RH development under these conditions. Functional analysis shows that such components of the RSL4-dependent transcriptional cascade influence the subsequent RH growth response to low temperature. These discoveries enhance our comprehension of how plants synchronize the RH growth in response to variations in temperature and nutrient availability at the cellular level.

show abstract

A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

et al. 2023

Self Cite

View full text Add to dashboard Cite

SUMMARYAdaptation to different soil conditions is a well‐regulated process vital for plant life. AtHB23 is a homeodomain‐leucine zipper I transcription factor (TF) that was previously revealed as crucial for plant survival under salinity conditions. We wondered whether this TF has partners to perform this essential function. Therefore, TF cDNA library screening, yeast two‐hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays were complemented with expression analyses and phenotypic characterization of silenced, mutant, overexpression, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and the salinity response. The encoding genes are coexpressed in specific root tissues and at specific developmental stages. In normal conditions, amiR68 silenced plants have fewer initiated roots, the opposite phenotype to that shown by amiR23 plants. AtMYB68 and AtPHL1 play opposite roles in lateral root elongation. Under salinity conditions, AtHB23 plays a crucial positive role in cooperating with AtMYB68, whereas AtPHL1 acts oppositely by obstructing the function of the former, impacting the plant's survival ability. Such interplay supports the complex interaction between these TF in primary and lateral roots. The root adaptation capability is associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival in salinity conditions.

show abstract

The Arabidopsis transcription factors AtPHL1 and AtHB23 act together promoting carbohydrate transport from pedicel-silique nodes to seeds

Cited by 4 publications

References 82 publications

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

RSL4 regulatory network acts as an early driver of root hair growth at low temperature inArabidopsis thaliana

A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

Contact Info

Product

Resources

About