The Arabidopsis thaliana NGATHA transcription factors negatively regulate cell proliferation of lateral organs

Lee, Byung Ha; Kwon, So Hyun; Lee, Sang Joo; Park, Soon Ki; Song, Jong Tae; Lee, Sangman; Lee, Myeong Min; Hwang, Yong-sic; Kim, Jeong Hoe

doi:10.1007/s11103-015-0386-y

Cited by 35 publications

(31 citation statements)

References 34 publications

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“…The Arabidopsis NGATHA genes ( NGA1 - NGA4 ) which belong to RAV family play important roles in leaf and flower development. nga1/nga2/nga3/nga4 quadruple mutant exhibits leaf shape defects and several flower defects (Alvarez et al, 2006; Lee et al, 2015). Matías-Hernández et al (2014) summarized the functions of RAV genes in controlling flowering in different pathways.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the RAV Family in Soybean and Functional Identification of GmRAV-03 Involvement in Salt and Drought Stresses and Exogenous ABA Treatment

Zhao

Zheng

et al. 2017

Front. Plant Sci.

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Transcription factors play vital roles in plant growth and in plant responses to abiotic stresses. The RAV transcription factors contain a B3 DNA binding domain and/or an APETALA2 (AP2) DNA binding domain. Although genome-wide analyses of RAV family genes have been performed in several species, little is known about the family in soybean (Glycine max L.). In this study, a total of 13 RAV genes, named as GmRAVs, were identified in the soybean genome. We predicted and analyzed the amino acid compositions, phylogenetic relationships, and folding states of conserved domain sequences of soybean RAV transcription factors. These soybean RAV transcription factors were phylogenetically clustered into three classes based on their amino acid sequences. Subcellular localization analysis revealed that the soybean RAV proteins were located in the nucleus. The expression patterns of 13 RAV genes were analyzed by quantitative real-time PCR. Under drought stresses, the RAV genes expressed diversely, up- or down-regulated. Following NaCl treatments, all RAV genes were down-regulated excepting GmRAV-03 which was up-regulated. Under abscisic acid (ABA) treatment, the expression of all of the soybean RAV genes increased dramatically. These results suggested that the soybean RAV genes may be involved in diverse signaling pathways and may be responsive to abiotic stresses and exogenous ABA. Further analysis indicated that GmRAV-03 could increase the transgenic lines resistance to high salt and drought and result in the transgenic plants insensitive to exogenous ABA. This present study provides valuable information for understanding the classification and putative functions of the RAV transcription factors in soybean.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the RAV Family in Soybean and Functional Identification of GmRAV-03 Involvement in Salt and Drought Stresses and Exogenous ABA Treatment

Zhao

Zheng

et al. 2017

Front. Plant Sci.

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“…NGAs and CINCINNATA-class-thermoresponsive cationic copolymers (CIN-TCPs) are co-expressed at many stages of leaf development, and their absence causes persistent marginal growth in leaves, cotyledons and floral organs [15]. Plants overexpressing individual Arabidopsis NGATHA genes (AtNGA1 to AtNGA4) displayed small, narrow lateral organs, and decreased cell numbers in the lateral organs in contrast to an nga1, nga2, nga3, nga4 quadruple mutant [16]. Arabidopsis plants overexpressing BrNGA1 also displayed markedly reduced lateral organ and root growth compared with growth in wild-type plants [17].…”

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confidence: 99%

Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

Guo

Wang

et al. 2020

IJMS

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Shoot branching is one of the most variable determinants of crop yield, and the signaling pathways of plant branches have become a hot research topic. As an important transcription factor in the B3 family, NGATHA1 (NGA1), plays an important role in regulating plant lateral organ development and hormone synthesis and transport, but few studies of the role of this gene in the regulation of plant growth and stress tolerance have been reported. In this study, the NGA1 gene was isolated from Medicago truncatula (Mt) and its function was characterized. The cis-acting elements upstream of the 5′ end of MtNGA1 and the expression pattern of MtNGA1 were analyzed, and the results indicated that the gene may act as a regulator of stress resistance. A plant expression vector was constructed and transgenic Arabidopsis plants were obtained. Transgenic Arabidopsis showed delayed flowering time and reduced branching phenotypes. Genes involved in the regulation of branching and flowering were differentially expressed in transgenic plants compared with wild-type plants. Furthermore, transgenic plants demonstrated strong tolerances to salt- and mannitol-induced stresses, which may be due to the upregulated expression of NCED3 (NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3) by the MtNGA1 gene. These results provide useful information for the exploration and genetic modification use of MtNGA1 in the future.

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“…Some members of the RAV family also possess an N-terminal AP2/ERF domain that recognizes the consensus sequence CAACA. RAV family members control flowering, organ growth, and have also been shown to be involved in leaf senescence, hormone signaling and responses to various stresses [19][20][21][22][23][24][25][26]. The ARF family proteins have an N-terminal B3 domain that recognizes the auxin response element TGTCTC in the promoter of genes responsive to auxin, followed by a highly divergent middle region that determines whether the ARFs act as an activator or repressor [27,28].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of the citrus B3 superfamily and their association with somatic embryogenesis

Liu

et al. 2020

Preprint

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Background: In citrus, genetic improvement via biotechnology is hindered by the obstacle of in vitro regeneration via somatic embryogenesis (SE). Although a few B3 transcription factors are reported to regulate embryogenesis, little is known about the B3 superfamily in citrus, and which members might be involved in SE.Results: Genome-wide sequence analysis identified 72 (CsB3) and 69 (CgB3) putative B3 superfamily members in the genomes of sweet orange (Citrus sinensis, polyembryonic) and pummelo (C. grandis, monoembryonic), respectively. Genome duplication analysis indicated that segmental and tandem duplication events contributed to the expansion of the B3 superfamily in citrus, and that the B3 superfamily evolved under the effect of purifying selection. Phylogenetic relationships were well supported by conserved gene structure and motifs outside the B3 domain, which allowed possible functions to be inferred by comparison with homologous genes from Arabidopsis. Expression analysis identified 23 B3 superfamily members that were expressed during SE in citrus and 17 that may play functional roles at late SE stages. Eight B3 genes were identified that were specific to the genome of polyembryonic sweet orange compared to monoembryonic pummelo. Of these eight B3 genes, CsARF19 was found to be specifically expressed at higher levels in embryogenic callus (EC), implying its possible involvement in EC initiation. Conclusions: This study provides a genome-wide analysis of the citrus B3 superfamily, including its genome organization, evolutionary features and expression profiles, and identifies specific family members that may be associated with SE.

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The Arabidopsis thaliana NGATHA transcription factors negatively regulate cell proliferation of lateral organs

Cited by 35 publications

References 34 publications

Genome-Wide Analysis of the RAV Family in Soybean and Functional Identification of GmRAV-03 Involvement in Salt and Drought Stresses and Exogenous ABA Treatment

Genome-Wide Analysis of the RAV Family in Soybean and Functional Identification of GmRAV-03 Involvement in Salt and Drought Stresses and Exogenous ABA Treatment

Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

Genome-wide analysis of the citrus B3 superfamily and their association with somatic embryogenesis

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