The Arabidopsis thaliana Class II Formin FH13 Modulates Pollen Tube Growth

Kollárová, Eva; Forero, Anežka Baquero; Cvrčková, Fatima

doi:10.3389/fpls.2021.599961

Cited by 14 publications

(5 citation statements)

References 66 publications

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“…In the networks modeled for the hybrids, genes with high betweenness were mostly associated with protein synthesis and gene expression regulation, including the protein translation factor SUI1 (Li et al 2022), the transcription factor BTF3 (Pruthvi et al 2017), and the calcium/calmodulin-regulated receptor-like kinase 1 (Yuan et al 2022). In contrast, the network modeled for S. spontaneum had genes with high betweenness primarily associated with cellular structure and division, such as the kinetochore-associated protein KNL-2 (Zuo et al 2022) and formin-like protein (Kollárová et al 2021). A high betweenness measure indicates that a gene permeates many gene associations, potentially facilitating the flow of interactions within the network.…”

Section: Discussionmentioning

confidence: 99%

Multiomic insights into sucrose accumulation in sugarcane

Aono,

Pimenta,

Diniz

et al. 2024

Preprint

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Sugarcane (Saccharumspp.) holds significant economic importance in sugar and biofuel production. Despite extensive research, understanding highly quantitative traits, such as sucrose content, remains challenging due to the complex genomic landscape of the crop. In this study, we conducted a multiomic investigation to elucidate the genetic architecture and molecular mechanisms governing sucrose accumulation in sugarcane. Using a biparental cross (IACSP95-3018 × IACSP93-3046) and a genetically diverse collection of sugarcane genotypes, we evaluated the soluble solids (Brix) and sucrose content (POL) across various years and environments. Both populations were genotyped using a genotyping-by-sequencing (GBS) approach, with single nucleotide polymorphisms (SNPs) identified via bioinformatics pipelines. Genotype‒phenotype associations were established using a combination of traditional linear mixed-effect models and machine learning algorithms. Furthermore, we conducted an RNA sequencing (RNA-Seq) experiment on genotypes exhibiting distinct Brix and POL profiles across different developmental stages. Differentially expressed genes (DEGs) potentially associated with variations in sucrose accumulation were identified. All findings were integrated through a comprehensive gene coexpression network analysis. Strong correlations among the evaluated characteristics were observed, with estimates of modest to high heritabilities. By leveraging a broad set of SNPs identified for both populations, we identified several SNPs potentially linked to phenotypic variance. Our examination of genes close to these markers facilitated the association of such SNPs with DEGs in genotypes with contrasting sucrose levels. Through the integration of these results with a gene coexpression network, we delineated a set of genes potentially involved in the regulatory mechanisms of sucrose accumulation in sugarcane, collectively contributing to the definition of this critical phenotype. Our findings constitute a significant resource for biotechnology and plant breeding initiatives. Furthermore, our genotype‒phenotype association models hold promise for application in genomic selection, offering valuable insights into the molecular underpinnings governing sucrose accumulation in sugarcane.

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Section: Discussionmentioning

confidence: 99%

Multiomic insights into sucrose accumulation in sugarcane

Aono,

Pimenta,

Diniz

et al. 2024

Preprint

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“…In Arabidopsis, loss of the pollen-expressed class II formin AtFH13, which localizes to intracytoplasmic (endomembrane?) structures, surprisingly stimulates pollen tube elongation, while its overexpression inhibits pollen tube growth ( Kollárová et al , 2021 ). In P. patens , the class II formin For2a localizes to endocytotic foci at the apices of tip-growing protonemata, where it drives the assembly of actin arrays used for myosin-dependent membrane vesicle delivery ( Vidali et al , 2009 ; van Gisbergen et al , 2012 , 2020 ; Galotto et al , 2021 ).…”

Section: Plant Class I Formins At the Expanding Plasmalemmamentioning

confidence: 99%

Transmembrane formins as active cargoes of membrane trafficking

Cvrčková,

Ghosh,

Kočová

2024

Journal of Experimental Botany

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Formins are a large, evolutionarily old family of plant cytoskeletal regulators whose roles include actin capping and nucleation, as well as modulation of microtubule dynamics. The plant class I formin clade is characterized by a unique domain organization, as most of its members are transmembrane proteins with possible cell wall-binding motifs exposed to the extracytoplasmic space – a structure that appears to be a synapomorphy of the plant kingdom. While such transmembrane formins are traditionally considered mainly as plasmalemma-localized proteins contributing to the organization of the cell cortex, we review, from a cell biology perspective, the growing evidence that they can also, at least temporarily, reside (and in some cases also function) in endomembranes including secretory and endocytotic pathway compartments, the endoplasmic reticulum, the nuclear envelope and the tonoplast. Based on this evidence, we propose that class I formins may thus serve as “active cargoes” of membrane trafficking, i.e. membrane-embedded proteins that modulate the fate of endo- or exocytotic compartments while being transported by them.

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“…Cold response annotated genes in the darkturquoise module included genes involved in mRNA splicing 55 , miRNA-mediated gene silencing and pollen development 56,57 , ABA signaling and post-translational modi cation of circadian clock proteins 58,59 . Epigenetic regulation of mRNA and protein levels in response to abiotic stress plays a role in regulating owering time 60 .…”

Section: Identi Cation Of Co-expressed Gene Modulesmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Candidate Genes For Cold Stress Response and Early Flowering in Pineapple

Yow

Laosuntisuk

Young

et al. 2023

Preprint

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Pineapple originates from tropical regions in South America and is therefore significantly impacted by cold stress. Periodic cold events in the equatorial regions where pineapple is grown may induce early flowering, also known as precocious flowering, resulting in monetary losses due to small fruit size and the need to make multiple passes for harvesting a single field. Currently, pineapple is one of the most important tropical fruits in the world in terms of consumption, and production losses caused by weather can have major impacts on worldwide exportation potential and economics. To further our understanding of and identify mechanisms for low-temperature tolerance in pineapple, and to identify the relationship between low-temperature stress and flowering time, we report here a transcriptomic analysis of two pineapple genotypes in response to low-temperature stress. Using meristem tissue collected from precocious flowering-susceptible MD2 and precocious flowering-tolerant Dole-17, we performed pairwise comparisons and weighted gene co-expression network analysis (WGCNA) to identify cold stress, genotype, and floral organ development-specific modules. Dole-17 had a greater increase in expression of genes that confer cold tolerance. The results suggested that low temperature stress in Dole-17 plants induces transcriptional changes to adapt and maintain homeostasis. Comparative transcriptomic analysis revealed differences in cuticular wax biosynthesis, carbohydrate accumulation, and vernalization-related gene expression between genotypes. Cold stress induced changes in ethylene and abscisic acid-mediated pathways differentially between genotypes, suggesting that MD2 may be more susceptible to hormone-mediated early flowering. The differentially expressed genes and module hub genes identified in this study are potential candidates for engineering cold tolerance in pineapple to develop new varieties capable of maintaining normal reproduction cycles under cold stress. In addition, a total of 461 core genes involved in development of reproductive tissues in pineapple were also identified in this study. This research provides an important genomic resource for understanding molecular networks underlying cold stress response and how cold stress affects flowering time in pineapple.

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The Arabidopsis thaliana Class II Formin FH13 Modulates Pollen Tube Growth

Cited by 14 publications

References 66 publications

Multiomic insights into sucrose accumulation in sugarcane

Multiomic insights into sucrose accumulation in sugarcane

Transmembrane formins as active cargoes of membrane trafficking

Comparative Transcriptome Analysis Reveals Candidate Genes For Cold Stress Response and Early Flowering in Pineapple

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