The Arabidopsis sweetie mutant is affected in carbohydrate metabolism and defective in the control of growth, development and senescence

Veyrès, Nicolas; Danon, Antoine; Aono, Mitsuko; Galliot, Sonia; Karibasappa, Yashoda Byrappa; Diet, Anouck; Grandmottet, François; Tamaoki, Masanori; Lesur, David; Pilard, Serge; Boitel-Conti, MichÃ ̈le; Sangwan‐Norreel, B. S.; Sangwan, Rajbir S.

doi:10.1111/j.1365-313x.2008.03541.x

Cited by 63 publications

(59 citation statements)

References 82 publications

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“…SWEETIE encodes a protein that may have a central role in sugar homeostasis 52 . In Arabidopsis, the sweetie mutant shows stunted growth, early senescence, flower sterility, and increased sugar levels.…”

Section: Candidate Gene Adaptation Correlates With Environmentmentioning

confidence: 99%

Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch

Salojärvi¹,

Smolander²,

Nieminen³

et al. 2017

Nat Genet

236

253

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Forest ecosystems maintain a large share of Northern Hemisphere biodiversity. Boreal forests comprise roughly 30% of global forest area 1 and contain the highest tree density among climate zones 2 . Moreover, boreal regions are undergoing extensive climate change. Annual temperature increases exceeding 1.5 °C are projected to result in a warming of 4-11 °C by the end of this century, with little concomitant increase in precipitation 1 . At this pace, climate zones will shift northward at a greater speed than trees can migrate 3 . To understand how future populations of forest trees may respond to climate change, it is essential to uncover past and present signatures of molecular adaptation in their genomes. Silver birch, B. pendula, is a pioneer species in boreal forests of Eurasia. Flowering of the species can be artificially accelerated 4 , giving it an advantage over other tree species with published genome sequences (such as poplar 5 , spruce 6 , and pine 7 ) for the optimization of fiber and biomass production.Here we sequenced 150 birch individuals and assembled a B. pendula reference genome from a fourth-generation inbred line, resulting in a high-quality assembly of 435 Mb that was linked to chromosomes using a dense genetic map. We analyzed SNPs in the genomes of 80 birch individuals spanning most of the geographic range of B. pendula, as well as seven other members of Betulaceae. Population genomic analyses of these data provide insights into the deep-time evolution of the birch family and on recent natural selection acting on silver birch.Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make birch an attractive model for forest biotechnology. We assembled and chromosomally anchored the nuclear genome of an inbred B. pendula individual. Gene duplicates from the paleohexaploid event were enriched for transcriptional regulation, whereas tandem duplicates were overrepresented by environmental responses. Population resequencing of 80 individuals showed effective population size crashes at major points of climatic upheaval. Selective sweeps were enriched among polyploid duplicates encoding key developmental and physiological triggering functions, suggesting that local adaptation has tuned the timing of and cross-talk between fundamental plant processes. Variation around the tightlylinked light response genes PHYC and FRS10 correlated with latitude and longitude and temperature, and with precipitation for PHYC. Similar associations characterized the growth-promoting cytokinin response regulator ARR1, and the wood development genes KAK and MED5A.A full list of affiliations appears at the end of the paper.

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Section: Candidate Gene Adaptation Correlates With Environmentmentioning

confidence: 99%

Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch

Salojärvi¹,

Smolander²,

Nieminen³

et al. 2017

Nat Genet

236

253

View full text Add to dashboard Cite

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“…This conclusion is supported by the observation that senescence was delayed compared with the wild type when, in T6P phosphatase, overexpresssors were challenged by external sugars. Although T6P levels were not determined in our study, a report by Veyres et al (2008) revealed accelerated senescence in the Arabidopsis sweetie mutant that contains elevated levels of T6P, trehalose, and starch (Veyres et al, 2008). Currently, however, the precise relation of T6P and trehalose during senescence remains open.…”

Section: Carbon Metabolism During Developmental Senescencementioning

confidence: 69%

Comprehensive Dissection of Spatiotemporal Metabolic Shifts in Primary, Secondary, and Lipid Metabolism during Developmental Senescence in Arabidopsis

et al. 2013

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Developmental senescence is a coordinated physiological process in plants and is critical for nutrient redistribution from senescing leaves to newly formed sink organs, including young leaves and developing seeds. Progress has been made concerning the genes involved and the regulatory networks controlling senescence. The resulting complex metabolome changes during senescence have not been investigated in detail yet. Therefore, we conducted a comprehensive profiling of metabolites, including pigments, lipids, sugars, amino acids, organic acids, nutrient ions, and secondary metabolites, and determined approximately 260 metabolites at distinct stages in leaves and siliques during senescence in Arabidopsis (Arabidopsis thaliana). This provided an extensive catalog of metabolites and their spatiotemporal cobehavior with progressing senescence. Comparison with silique data provides clues to source-sink relations. Furthermore, we analyzed the metabolite distribution within single leaves along the basipetal sink-source transition trajectory during senescence. Ceramides, lysolipids, aromatic amino acids, branched chain amino acids, and stressinduced amino acids accumulated, and an imbalance of asparagine/aspartate, glutamate/glutamine, and nutrient ions in the tip region of leaves was detected. Furthermore, the spatiotemporal distribution of tricarboxylic acid cycle intermediates was already changed in the presenescent leaves, and glucosinolates, raffinose, and galactinol accumulated in the base region of leaves with preceding senescence. These results are discussed in the context of current models of the metabolic shifts occurring during developmental and environmentally induced senescence. As senescence processes are correlated to crop yield, the metabolome data and the approach provided here can serve as a blueprint for the analysis of traits and conditions linking crop yield and senescence.

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“…While an effect of increased trehalose formation due to otsB expression on senescence cannot be fully excluded, this is unlikely, because plants expressing a functional Escherichia coli trehalase, TreF, flower and senesce at the same time as the wild type (Schluepmann et al, 2003). Accelerated senescence of an Arabidopsis mutant that contains increased amounts of T6P, trehalose, and other sugars (Veyres et al, 2008) supports the link between carbon availability, T6P, and senescence shown here.…”

Section: Decreased T6p Delays the Onset Of Leaf Senescencementioning

confidence: 72%

Trehalose 6-Phosphate Is Required for the Onset of Leaf Senescence Associated with High Carbon Availability

et al. 2012

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Trehalose 6-phosphate (T6P) is an important regulator of plant metabolism and development. T6P content increases when carbon availability is high, and in young growing tissue, T6P inhibits the activity of Snf1-related protein kinase (SnRK1). Here, strong accumulation of T6P was found in senescing leaves of Arabidopsis (Arabidopsis thaliana), in parallel with a rise in sugar contents. To determine the role of T6P in senescence, T6P content was altered by expressing the bacterial T6P synthase gene, otsA (to increase T6P), or the T6P phosphatase gene, otsB (to decrease T6P). In otsB-expressing plants, T6P accumulated less strongly during senescence than in wild-type plants, while otsA-expressing plants contained more T6P throughout. Mature otsB-expressing plants showed a similar phenotype as described for plants overexpressing the SnRK1 gene, KIN10, including reduced anthocyanin accumulation and delayed senescence. This was confirmed by quantitative reverse transcriptionpolymerase chain reaction analysis of senescence-associated genes and genes involved in anthocyanin synthesis. To analyze if the senescence phenotype was due to decreased sugar sensitivity, the response to sugars was determined. In combination with low nitrogen supply, metabolizable sugars (glucose, fructose, or sucrose) induced senescence in wild-type and otsA-expressing plants but to a smaller extent in otsB-expressing plants. The sugar analog 3-O-methyl glucose, on the other hand, did not induce senescence in any of the lines. Transfer of plants to and from glucose-containing medium suggested that glucose determines senescence during late development but that the effects of T6P on senescence are established by the sugar response of young plants.In plants, the disaccharide trehalose is synthesized by the conversion of UDP-Glc and Glc-6-P to trehalose 6-phosphate (T6P) in a reaction catalyzed by T6P synthase (TPS), followed by hydrolysis of T6P to trehalose in a reaction catalyzed by T6P phosphatase (TPP). Since the identification of functional TPS and TPP genes in Arabidopsis (Arabidopsis thaliana; Blázquez et al., 1998;Vogel et al., 1998), the role of trehalose metabolism in plants has received increasing attention. Evidence has accumulated suggesting a role for the precursor of trehalose, T6P, as a signal for the regulation of plant metabolism and development (for review, see Eastmond and Graham, 2003;

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The Arabidopsis sweetie mutant is affected in carbohydrate metabolism and defective in the control of growth, development and senescence

Cited by 63 publications

References 82 publications

Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch

Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch

Comprehensive Dissection of Spatiotemporal Metabolic Shifts in Primary, Secondary, and Lipid Metabolism during Developmental Senescence in Arabidopsis

Trehalose 6-Phosphate Is Required for the Onset of Leaf Senescence Associated with High Carbon Availability

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