The Arabidopsis splicing regulator SR45 confers salt tolerance in a splice isoform-dependent manner

Albaqami, Mohammed; Laluk, Kristin; Reddy, Anireddy S. N.

doi:10.1007/s11103-019-00864-4

Cited by 51 publications

(56 citation statements)

References 63 publications

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“…Consistent with this notion, the two isoforms of At-SR45, which differ by an insertion of eight amino acids in At-SR45.1 that replaces an arginine in At-SR45.2, complement different developmental phenotypes in the at-sr45 mutant (Zhang and Mount, 2009). Moreover, only At-SR45.1 is involved in plant tolerance against salinity stress (Albaqami et al, 2019).…”

Section: Regulation Of Tomato Sr Protein-coding Genes In Different Tissuesmentioning

confidence: 65%

Identification and Regulation of Tomato Serine/Arginine-Rich Proteins Under High Temperatures

et al. 2021

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Alternative splicing is an important mechanism for the regulation of gene expression in eukaryotes during development, cell differentiation or stress response. Alterations in the splicing profiles of genes under high temperatures that cause heat stress (HS) can impact the maintenance of cellular homeostasis and thermotolerance. Consequently, information on factors involved in HS-sensitive alternative splicing is required to formulate the principles of HS response. Serine/arginine-rich (SR) proteins have a central role in alternative splicing. We aimed for the identification and characterization of SR-coding genes in tomato (Solanum lycopersicum), a plant extensively used in HS studies. We identified 17 canonical SR and two SR-like genes. Several SR-coding genes show differential expression and altered splicing profiles in different organs as well as in response to HS. The transcriptional induction of five SR and one SR-like genes is partially dependent on the master regulator of HS response, HS transcription factor HsfA1a. Cis-elements in the promoters of these SR genes were predicted, which can be putatively recognized by HS-induced transcription factors. Further, transiently expressed SRs show reduced or steady-state protein levels in response to HS. Thus, the levels of SRs under HS are regulated by changes in transcription, alternative splicing and protein stability. We propose that the accumulation or reduction of SRs under HS can impact temperature-sensitive alternative splicing.

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Section: Regulation Of Tomato Sr Protein-coding Genes In Different Tissuesmentioning

confidence: 65%

Identification and Regulation of Tomato Serine/Arginine-Rich Proteins Under High Temperatures

et al. 2021

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“…In addition to U1 snRNP 70K, several other SR proteins, including SC34‐like (SR33), RSZ1, SR30, SR34, and spliceosomal components SKIP and CACTIN, interact with SR45 to regulate pre‐mRNA splicing (Golovkin and Reddy, ; Mayeda et al , ; Golovkin and Reddy, ; Carvalho et al , ; Wang et al , ; Baldwin et al , ; Stankovic et al , ). Recent studies have indicated that SR45 plays an essential role in plant growth and adaptation to environment stress (Carvalho et al , ; Xing et al , ; Carvalho and Szakonyi, ; Albaqami et al , ). However, the precise molecular mechanisms of SR45 underlying pre‐mRNA splicing and mRNA quality control in response to abiotic stress remain unclear.…”

Section: Introductionmentioning

confidence: 99%

OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

et al. 2020

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Sessile plants have evolved distinct mechanisms to respond and adapt to adverse environmental conditions through diverse mechanisms including RNA processing. While the role of RNA processing in the stress response is well understood for Arabidopsis thaliana, limited information is available for rice (Oryza sativa).Here, we show that OsFKBP20-1b, belonging to the immunophilin family, interacts with the splicing factor OsSR45 in both nuclear speckles and cytoplasmic foci, and plays an essential role in post-transcriptional regulation of abiotic stress response. The expression of OsFKBP20-1b was highly upregulated under various abiotic stresses. Moreover genetic analysis revealed that OsFKBP20-1b positively affected transcription and pre-mRNA splicing of stress-responsive genes under abiotic stress conditions. In osfkbp20-1b loss-of-function mutants, the expression of stress-responsive genes was downregulated, while that of their splicing variants was increased. Conversely, in plants overexpressing OsFKBP20-1b, the expression of the same stress-responsive genes was strikingly upregulated under abiotic stress. In vivo experiments demonstrated that OsFKBP20-1b directly maintains protein stability of OsSR45 splicing factor. Furthermore, we found that the plant-specific OsFKBP20-1b gene has uniquely evolved as a paralogue only in some Poaceae species. Together, our findings suggest that OsFKBP20-1b-mediated RNA processing contributes to stress adaptation in rice.OsFKBP20-1b is involved in pre-mRNA processing under abiotic stress 999 Subcellular localization and co-localizationThe full-length coding sequence (CDS) of OsFKBP20-1b was cloned into the pCAMBIA1302 binary vector, and the recombinant

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“…Genetic analysis revealed that SR45.1-GFP complements the flower petal e1917170-6 and salt-sensitive phenotypes, but not the root growth. 72,73 Conversely, SR45.2-GFP only rescues root growth, and not others. 73 Interestingly, both isoforms are able to rescue the hypersensitivity to glucose and ABA.…”

Section: Alternative Splicing In Floral Organs Regulationmentioning

confidence: 98%

“… 71 SR45 , encoding a unique plant SR protein, was identified to yield the two transcripts of SR45.1 and SR45.2 , which have distinct roles during normal plant development and salt stress. 72 , 73 Compared with SR45.1, SR45.2 replaces eight amino acids (TSPQRKTG) with a single arginine by the alternative usage of 21 nucleotides in 3ʹ splice site. Loss-of-function mutant sr45–1 , which affects both isoforms, shows several developmental defects, including defects in petal development, root growth, and salt tolerance.…”

Section: Involvement Of Alternative Splicing In Plant Development and Stress Responsementioning

confidence: 99%

Normal, novel or none: versatile regulation from alternative splicing

Liu

Tang

Liu

et al. 2021

Plant Signaling & Behavior

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Pre-mRNA splicing is a vital step in the posttranscriptional regulation of gene expression. Splicing is catalyzed by the spliceosome, a multidalton RNA–protein complex, through two successive transesterifications to yield mature mRNAs. In Arabidopsis, more than 61% of all transcripts from intron-containing genes are alternatively spliced, thereby resulting in transcriptome and subsequent proteome diversities for cellular processes. Moreover, it is estimated that more alternative splicing (AS) events induced by adverse stimuli occur to confer stress tolerance. Recently, increasing AS variants encoding normal or novel proteins, or degraded by nonsense-mediated decay (NMD) and their corresponding splicing factors or regulators acting at the posttranscriptional level have been functionally characterized. This review comprehensively summarizes and highlights the advances in our understanding of the biological functions and underlying mechanisms of AS events and their regulators in Arabidopsis and provides prospects for further research on AS in crops.

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The Arabidopsis splicing regulator SR45 confers salt tolerance in a splice isoform-dependent manner

Cited by 51 publications

References 63 publications

Identification and Regulation of Tomato Serine/Arginine-Rich Proteins Under High Temperatures

Identification and Regulation of Tomato Serine/Arginine-Rich Proteins Under High Temperatures

OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

Normal, novel or none: versatile regulation from alternative splicing

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