The Arabidopsis MIEL1 E3 ligase negatively regulates ABA signalling by promoting protein turnover of MYB96

Lee, Hong Gil; Seo, Pil Joon

doi:10.1038/ncomms12525

Cited by 82 publications

(68 citation statements)

References 63 publications

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“…Co‐immunoprecipitation experiments in seedlings of Arabidopsis were performed as previously described, with a few modifications (Lee and Seo, ). Up to 1 g of seedlings was homogenized in a mortar with grinding buffer [50 m m Tris‐HCl (pH 7.5), 150 m m NaCl, 10 m m MgCl 2 , 0.1% Nonidet P‐40, 1 m m phenylmethylsulfonylfluoride, and protease inhibitors].…”

Section: Methodsmentioning

confidence: 99%

MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore complex, affects development through cell division in Arabidopsis thaliana

et al. 2018

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Kinetochore, a protein super-complex on the centromere of chromosomes, mediates chromosome segregation during cell division by providing attachment sites for spindle microtubules. The NDC80 complex, composed of four proteins, NDC80, NUF2, SPC24 and SPC25, is localized at the outer kinetochore and connects spindle fibers to the kinetochore. Although it is conserved across species, functional studies of this complex are rare in Arabidopsis. Here, we characterize a recessive mutant, meristem unstructured-1 (mun-1), exhibiting an abnormal phenotype with unstructured shoot apical meristem caused by ectopic expression of the WUSCHEL gene in unexpected tissues. mun-1 is a weak allele because of the insertion of T-DNA in the promoter region of the SPC24 homolog. The mutant exhibits stunted growth, embryo arrest, DNA aneuploidy, and defects in chromosome segregation with a low cell division rate. Null mutants of MUN from TALEN and CRISPR/Cas9-mediated mutagenesis showed zygotic embryonic lethality similar to nuf2-1; however, the null mutations were fully transmissible via pollen and ovules. Interactions among the components of the NDC80 complex were confirmed in a yeast two-hybrid assay and in planta co-immunoprecipitation. MUN is co-localized at the centromere with HTR12/CENH3, which is a centromere-specific histone variant, but MUN is not required to recruit HTR12/CENH3 to the kinetochore. Our results support that MUN is a functional homolog of SPC24 in Arabidopsis, which is required for proper cell division. In addition, we report the ectopic generations of stem cell niches by the malfunction of kinetochore components.

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Section: Methodsmentioning

confidence: 99%

MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore complex, affects development through cell division in Arabidopsis thaliana

et al. 2018

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“…Co-IP assays were performed as previously described by Lee and Seo (2016) with a few modifications. Two grams of 10-day-old WT and CsVp::TAF15b-GFP seedlings grown under long-day conditions were ground in liquid nitrogen and 2 mL of grinding buffer were added to each sample.…”

Section: Western Blot and Co-immunoprecipitation (Co-ip)mentioning

confidence: 99%

TAF15b, involved in the autonomous pathway for flowering, represses transcription of FLOWERING LOCUS C

et al. 2017

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SUMMARYTATA-binding protein-associated factors (TAFs) are general transcription factors within the transcription factor IID (TFIID) complex, which recognizes the core promoter of genes. In addition to their biochemical function, it is known that several TAFs are involved in the regulation of developmental processes. In this study, we found that TAF15b affects flowering time, especially through the autonomous pathway (AP) in Arabidopsis. The mutant taf15b shows late flowering compared with the wild type plant during both long and short days, and vernalization accelerates the flowering time of taf15b. In addition, taf15b shows strong upregulation of FLOWERING LOCUS C (FLC), a flowering repressor in Arabidopsis, and the flc taf15b double mutant completely offsets the late flowering of taf15b, indicating that TAF15b is a typical AP gene. The taf15b mutant also shows increased transcript levels of COOLAIR, an antisense transcript of FLC. Consistently, chromatin immunoprecipitation (ChIP) analyses showed that the TAF15b protein is enriched around both sense and antisense transcription start sites of the FLC locus. In addition, co-immunoprecipitation showed that TAF15b interacts with RNA polymerase II (Pol II), while ChIP showed increased enrichment of the phosphorylated forms, both serine 2 (Ser2) and Ser5, of the C-terminal domain of Pol II at the FLC locus, which is indicative of transcriptional elongation. Finally, taf15b showed higher enrichment of the active histone marker, H3K4me3, on FLC chromatin. Taken together, our results suggest that TAF15b affects flowering time through transcriptional repression of FLC in Arabidopsis.

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“…Ubiquitination, a type of post-translational protein modification, plays an important role in plant growth and abiotic stress responses (Shu and Yang, 2017), and ubiquitination of MYB TFs currently attracts interest in a variety of fields. In Arabidopsis, the RING-type E3 ligase MYB30-INTERACTING E3 LIGASE 1 (MIEL1) affects the protein abundance of MYB30 and MYB96 (Marino et al, 2013;Lee and Seo, 2016). In apple, the degradation of MdMYB1 protein is mediated by MdCOP1 and MdMIEL1 (Li et al, 2012;An et al, 2017b).…”

Section: Introductionmentioning

confidence: 99%

R2R3‐MYB transcription factor MdMYB23 is involved in the cold tolerance and proanthocyanidin accumulation in apple

et al. 2018

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Cold stress severely affects plant growth and yield. C-repeat binding factors (CBFs) play important roles in the response to cold stress. In the present study, we identified an R2R3-MYB transcription factor (TF) MdMYB23 from apple (Malus × domestic) using transcriptome analyses, which was notably induced in response to cold stress. Transgenic apple calli and Arabidopsis with overexpression of MdMYB23 exhibited increased cold tolerance. Electrophoretic mobility shift assay (EMSA) and transient expression assays indicated that MdMYB23 directly bound to the promoters of MdCBF1 and MdCBF2 and activated their expression. MdMYB23 interacted with the promoter of MdANR, a key modulator of proanthocyanidin biosynthesis, and activated its expression to promote proanthocyanidin accumulation and reactive oxygen species (ROS) scavenging. MdBT2 was identified as an MdMYB23-interacting protein using yeast two-hybrid (Y2H), pull-down, and bimolecular fluorescence complementation (BiFC) assays. MdBT2 repressed cold tolerance and proanthocyanidin accumulation by promoting the degradation of MdMYB23 protein. Our findings shed light on the functions of MYB TFs and underlying mechanism in the modulation of plant cold tolerance.

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The Arabidopsis MIEL1 E3 ligase negatively regulates ABA signalling by promoting protein turnover of MYB96

Cited by 82 publications

References 63 publications

MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore complex, affects development through cell division in Arabidopsis thaliana

MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore complex, affects development through cell division in Arabidopsis thaliana

TAF15b, involved in the autonomous pathway for flowering, represses transcription of FLOWERING LOCUS C

R2R3‐MYB transcription factor MdMYB23 is involved in the cold tolerance and proanthocyanidin accumulation in apple

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