The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

Malek, B. von; Graaff, Eric van der; Schneitz, Kay; Keller, Beat

doi:10.1007/s00425-002-0906-2

Cited by 273 publications

(254 citation statements)

References 28 publications

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“…In addition the sequence of the ps-2 gene will give us more insights into the physiology of anther dehiscence in tomato. Similar phenotypes have been observed in some Arabidopsis mutants where, in most of the cases, the jasmonic acid pathway was involved (Feys et al 1994;Ishiguro et al 2001;von Malek et al 2002;McConn and Browse 1996;Park et al 2002;Sanders et al 2000;Stintzi and Browse 2000;Xie et al 1998). Once the ps-2 gene is cloned, it will be interesting to know whether this gene is ortholog to one of those genes already identiWed in Arabidopsis.…”

Section: At(t0891)supporting

confidence: 59%

“…The molecular mechanism of ps-2 in tomato is unknown, but similar mutant phenotypes have been observed and studied mainly in Arabidopsis, such as the mutant myb26 (Steiner-Lange et al 2003), the mutants coi1 (Feys et al 1994;Xie et al 1998) and dad1 (Ishiguro et al 2001), the double mutants opr3/ dde1 (Sanders et al 2000;Stintzi and Browse 2000) and aos/dde2-2 (von Malek et al 2002;Park et al 2002) and the triple mutant fad3/fad7/fad8 (McConn and Browse 1996). They are characterized by defects in Wlament elongation, timing of anther dehiscence and often show reduced pollen viability.…”

Section: Introductionmentioning

confidence: 98%

“…All, except the mutant myb26, are in one way or another involved in the jasmonic acid pathway. The mutant coi1 (Xie et al 1998) is insensitive to jasmonic acid, while the others are defective in jasmonic acid synthesis (Feys et al 1994;Ishiguro et al 2001;McConn and Browse 1996;Park et al 2002;Sanders et al 2000;Stintzi and Browse 2000;von Malek et al 2002). Atanassova (1999) observed up to 26% self-pollination in the most extreme cases, on cultivar "Vrbicanske nizke" (ps-2/ps-2) under Weld conditions.…”

Section: Introductionmentioning

confidence: 99%

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High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers

et al. 2006

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Functional male sterility is an important trait for the production of hybrid seeds. Among the genes coding for functional male sterility in tomato is the positional sterility gene ps-2. ps-2 is monogenic recessive, confers non-dehiscent anthers and is the most suitable for practical uses. In order to have tools for molecularassisted selection (MAS) we Wne mapped the ps-2 locus. This was done in an F 2 segregating population derived from the interspeciWc cross between a functionally male sterile line (ps-2/ps-2; Solanum lycopersicum) and a functionally male fertile line (S. pimpinellifolium). Here we report the procedure that has led to the high-resolution Wne mapping of the ps-2 locus in a 1.65 cM interval delimited by markers T0958 and T0635 on the short arm of Chromosome 4. The presence of many COS markers in the local high-resolution map allowed us to study the synteny between tomato and Arabidopsis at the ps-2 locus region. No obvious candidate gene for ps-2 was identiWed among the known functional male sterility genes in Arabidopsis.

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Section: At(t0891)supporting

confidence: 59%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers

et al. 2006

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“…In our analysis, we use the JA-biosynthesis mutant aos, in which the single Allene Oxide Synthase (AOS) gene in Arabidopsis has been knocked out (Park et al, 2002) and the production of JA and its precursors is impaired (von Malek et al, 2002). In the aos mutant, JA downstream signaling can be rescued by exogenous JAs.…”

mentioning

confidence: 99%

Jasmonate Controls Leaf Growth by Repressing Cell Proliferation and the Onset of Endoreduplication while Maintaining a Potential Stand-By Mode

et al. 2013

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Phytohormones regulate plant growth from cell division to organ development. Jasmonates (JAs) are signaling molecules that have been implicated in stress-induced responses. However, they have also been shown to inhibit plant growth, but the mechanisms are not well understood. The effects of methyl jasmonate (MeJA) on leaf growth regulation were investigated in Arabidopsis (Arabidopsis thaliana) mutants altered in JA synthesis and perception, allene oxide synthase and coi1-16B (for coronatine insensitive1), respectively. We show that MeJA inhibits leaf growth through the JA receptor COI1 by reducing both cell number and size. Further investigations using flow cytometry analyses allowed us to evaluate ploidy levels and to monitor cell cycle progression in leaves and cotyledons of Arabidopsis and/or Nicotiana benthamiana at different stages of development. Additionally, a novel global transcription profiling analysis involving continuous treatment with MeJA was carried out to identify the molecular players whose expression is regulated during leaf development by this hormone and COI1. The results of these studies revealed that MeJA delays the switch from the mitotic cell cycle to the endoreduplication cycle, which accompanies cell expansion, in a COI1-dependent manner and inhibits the mitotic cycle itself, arresting cells in G1 phase prior to the S-phase transition. Significantly, we show that MeJA activates critical regulators of endoreduplication and affects the expression of key determinants of DNA replication. Our discoveries also suggest that MeJA may contribute to the maintenance of a cellular "stand-by mode" by keeping the expression of ribosomal genes at an elevated level. Finally, we propose a novel model for MeJA-regulated COI1-dependent leaf growth inhibition.

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“…Unlike aim1 (Richmond and Bleecker, 1999), floral morphology was normal in cts mutants, although they exhibited delayed filament extension (Fig. 1B), a phenotype that is shared by plants lacking functional allene oxide synthase and COI1, which are required for JA biosynthesis and perception, respectively (Feys et al, 1994;Park et al, 2002;von Malek et al, 2002). However, application of JA did not rescue the anther extension phenotype of cts flowers (data not shown).…”

Section: Discussion Cts Mutants Have a Subtle Vegetative And Floral Pmentioning

confidence: 92%

The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis

et al. 2007

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COMATOSE (CTS) encodes a peroxisomal ATP-binding cassette transporter required not only for b-oxidation of storage lipids during germination and establishment, but also for biosynthesis of jasmonic acid and conversion of indole butyric acid to indole acetic acid. cts mutants exhibited reduced fertilization, which was rescued by genetic complementation, but not by exogenous application of jasmonic acid or indole acetic acid. Reduced fertilization was also observed in thiolase (kat2-1) and peroxisomal acyl-Coenzyme A synthetase mutants (lacs6-1,lacs7-1), indicating a general role for b-oxidation in fertility. Genetic analysis revealed reduced male transmission of cts alleles and both cts pollen germination and tube growth in vitro were impaired in the absence of an exogenous carbon source. Aniline blue staining of pollinated pistils demonstrated that pollen tube growth was affected only when both parents bore the cts mutation, indicating that expression of CTS in either male or female tissues was sufficient to support pollen tube growth in vivo. Accordingly, abundant peroxisomes were detected in a range of maternal tissues. Although g-aminobutyric acid levels were reduced in flowers of cts mutants, they were unchanged in kat2-1, suggesting that alterations in g-aminobutyric acid catabolism do not contribute to the reduced fertility phenotype through altered pollen tube targeting. Taken together, our data support an important role for b-oxidation in fertility in Arabidopsis (Arabidopsis thaliana) and suggest that this pathway could play a role in the mobilization of lipids in both pollen and female tissues.

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The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

Cited by 273 publications

References 28 publications

High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers

High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers

Jasmonate Controls Leaf Growth by Repressing Cell Proliferation and the Onset of Endoreduplication while Maintaining a Potential Stand-By Mode

The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis

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