The Arabidopsis<i>Somatic Embryogenesis Receptor Kinase 1</i>Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in Culture

Hecht, Valérie; Vielle‐Calzada, Jean‐Philippe; Hartog, Marijke; Schmidt, E.D.L.; Boutilier, Kim; Grossniklaus, Ueli; Vries, S.C. de

doi:10.1104/pp.010324

Cited by 586 publications

(406 citation statements)

References 79 publications

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“…The identities of the BBM target genes do not provide insight into the specific pathways activated by this transcription factor, as to our knowledge none of the genes, with the exception of XTH9, have been functionally characterized, and most of the BBM targets belong to large multigene families, the members of which are involved in diverse processes. None of the immediate BBM target genes correspond to any genes known to induce (LEC1, Lotan et al 1998;LEC2, Stone et al 2001;WUS, Zuo et al 2002) or enhance (SERK1, Hecht et al 2001;AGL15, Harding et al 2003) somatic embryogenesis in Arabidopsis. There appear to be many routes through which seedlings can be reprogrammed to form embryos, thus it is likely that these different pathways eventually merge to direct the same developmental outcome.…”

Section: Discussionmentioning

confidence: 99%

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Passarinho¹,

Ketelaar²,

Xing³

et al. 2008

Plant Mol Biol

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103

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Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth.

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Section: Discussionmentioning

confidence: 99%

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Passarinho¹,

Ketelaar²,

Xing³

et al. 2008

Plant Mol Biol

Self Cite

103

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“…Several 'best-guess' genes for inducing embryogenesis without fertilization have been identified, such as AtSERK1 (this is expressed in pre-fertilization ovules and early embryos, and overexpression in vivo increases the embryogenic potential of callus (Hecht et al 2001)) and BABY BOOM (expressed during seed development and overexpression induces embryogenesis on parts of the plant such as leaf margins and shoot apex (Boutilier et al 2002)). Although the ectopic expression of these genes stimulates somatic embryogenesis in culture or on vegetative organs, there is so far no evidence that they would induce parthenogenesis within a seed, which is a requirement for apomixis.…”

Section: Engineering Apomixismentioning

confidence: 99%

Genetic mechanisms of apomixis

Spielman

Vinkenoog

Scott

2003

Phil. Trans. R. Soc. Lond. B

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The introduction of apomixis to crops would allow desirable genotypes to be propagated while preventing undesirable gene flow, but so far there has been little success in transferring this trait from a natural apomict to another species. One explanation is the sensitivity of endosperm to changes in relative maternal and paternal contribution owing to parental imprinting, an epigenetic system of transcriptional regulation by which some genes are expressed from only the maternally or paternally contributed allele. In sexual species, endosperm typically requires a ratio of two maternal genomes to one paternal genome for normal development, but this ratio is often altered in apomicts, suggesting that the imprinting system is altered as well. We present evidence that modification of DNA methylation is one mechanism by which the imprinting system could be altered to allow endosperm development in apomicts. Another feature of natural apomixis is the modification of the normal fertilization programme. Sexual reproduction uses both sperm from each pollen grain, but pseudogamous apomicts, which require a sexual endosperm to support the asexual embryo, often use just one. We present evidence that multiple fertilization of the central cell is possible in Arabidopsis thaliana , suggesting that pseudogamous apomicts may also need to acquire a mechanism for preventing more than one sperm from contributing to the endosperm. We conclude that strategies to transfer apomixis to crop species should take account of endosperm development and particularly its sensitivity to parental imprinting, as well as the mechanism of fertilization.

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“…The carrot SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) gene was shown to mark vegetative-to-embryonic transition in carrot suspension cell cultures [13], and its Arabidopsis orthologous gene was found to enhance embryogenic competence in cell cultures as well [14]. The BABY BOOM (BBM) gene, encoding an AP2/ERF-type transcription factor, was identified from Brassica napus and Arabidopsis thaliana.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

et al. 2008

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Formation of somatic embryos from non-germline cells is unique to higher plants and can be manipulated in a variety of species. Previous studies revealed that overexpression of several Arabidopsis genes, including WUSCHEL (WUS)/PLANT GROWTH ACTIVATOR6 (PGA6), BABY BOOM, LEAFY COTYLEDON1 (LEC1), and LEC2, is able to cause vegetative-to-embryonic transition or the formation of somatic embryos. Here, we report that a gain-offunction mutation in the Arabidopsis PGA37 gene, encoding the MYB118 transcription factor, induced vegetative-toembryonic transition, the formation of somatic embryos from root explants, and an elevated LEC1 expression level. Double mutant analysis showed that WUS was not required for induction of somatic embryos by PGA37/MYB118. In addition, overexpression of MYB115, a homolog of PGA37/MYB118, caused a pga37-like phenotype. A myb118 myb115 double mutant did not show apparent developmental abnormalities. Collectively, these results suggest that PGA37/ MYB118 and MYB115 promote vegetative-to-embryonic transition, through a signaling pathway independent of WUS.

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The ArabidopsisSomatic Embryogenesis Receptor Kinase 1Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in Culture

Cited by 586 publications

References 79 publications

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Genetic mechanisms of apomixis

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

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