The Arabidopsis heat‐intolerant 5 (hit5)/enhanced response to aba 1 (era1) mutant reveals the crucial role of protein farnesylation in plant responses to heat stress

Wu, Jia Rong; Wang, Lian Chin; Lin, Yun-Huin; Weng, Chi Pei; Yeh, Chien-Hung; Wu, Shaw Jye

doi:10.1111/nph.14212

Cited by 40 publications

(16 citation statements)

References 64 publications

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“…The expression of heat shock protein (HSP) and heat shock factor (HSF) genes serves as a master regulator of heat stress responses (HSR). To determine the relationship between SlMPK1 and HSR, we measured the transcript levels of HSFA2 and HSP101, the major heat-inducible HSF and HSP genes (Wu et al, 2017). Our results show that the expression of both genes did not change significantly in SlMPK1 RNAi lines when compared with the wild type at normal growth temperature or HT stress (Supplemental Fig.…”

Section: Suppression Of Slmpk1 Increased Ht Tolerance In Transgenic Pmentioning

confidence: 88%

The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response

Ding

et al. 2018

Plant Physiology

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High-temperature (HT) stress is a major environmental stress that limits plant growth and development. MAPK cascades play key roles in plant growth and stress signaling, but their involvement in the HT stress response is poorly understood. Here, we describe a 47-kD MBP-phosphorylated protein (p47-MBPK) activated in tomato () leaves under HT and identify it as SlMPK1 by tandem mass spectrometry analysis. Silencing of in transgenic tomato plants resulted in enhanced tolerance to HT, while overexpression resulted in reduced tolerance. Proteomic analysis identified a set of proteins involved in antioxidant defense that are significantly more abundant in RNA interference- plants than nontransgenic plants under HT stress. RNA interference- plants also showed changes in membrane lipid peroxidation and antioxidant enzyme activities. Furthermore, using yeast two-hybrid screening, we identified a serine-proline-rich protein homolog, SlSPRH1, which interacts with SlMPK1 in yeast, in plant cells, and in vitro. We demonstrate that SlMPK1 can directly phosphorylate SlSPRH1. Furthermore, the serine residue serine-44 of SlSPRH1 is a crucial phosphorylation site in the SlMPK1-mediated antioxidant defense mechanism activated during HT stress. We also demonstrate that heterologous expression of in Arabidopsis () led to a decrease in thermotolerance and lower antioxidant capacity. Taken together, our results suggest that SlMPK1 is a negative regulator of thermotolerance in tomato plants. SlMPK1 acts by regulating antioxidant defense, and its substrate SlSPRH1 is involved in this pathway.

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Section: Suppression Of Slmpk1 Increased Ht Tolerance In Transgenic Pmentioning

confidence: 88%

The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response

Ding

et al. 2018

Plant Physiology

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“…Exogenous application of ABA significantly maintained higher seedling growth of chickpea ( Cicer arietinum ) at high temperature, by enhancing the endogenous levels of ABA and osmolytes, while this was suppressed in the presence of its biosynthetic inhibitor fluridone (Kumar et al ). Arabidopsis mutants deficient in ABA synthesis and signaling were more susceptible to heat stress than wild‐type (WT) plants (Larkindale et al , Wu et al ). Zandalinas et al () showed that ABA was crucial for the accumulation of key proteins involved in stress acclimation and regulated stomatal closure under heat stress, such as heat shock transcription factors (HSFs) and heat shock proteins (HSPs).…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid mediation of drought priming‐enhanced heat tolerance in tall fescue (Festuca arundinacea) and Arabidopsis

Zhang

Wang

Zhuang

et al. 2019

Physiologia Plantarum

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Abscisic acid (ABA) may play roles in mediating cross stress tolerance in plants. The objectives of this study were to investigate the priming effects of drought and ABA on heat tolerance and to determine how ABA may be involved in enhanced heat tolerance by drought. Focusing on the transcriptional level, two independent experiments were conducted, using a perennial grass species, tall fescue (Festuca arundinacea) and Arabidopsis. In experiment 1, tall fescue plants were exposed to mild drought by withholding irrigation for 8 days (drought priming) and foliar sprayed with ABA or an ABA-synthesis inhibitor (fluridone). After that they were subsequently subjected to heat stress (38/33 ∘ C day/night) for 25 days in growth chambers. In experiment 2, Arabidopsis Columbia ecotype (wild-type) and ABA-deficient mutant (aba3-1, CS157) were pre-treated with drought priming and then exposed to heat stress (45/40 ∘ C) for 3 days. The physiological analysis demonstrated that both drought priming and foliar application of ABA-enhanced heat tolerance in tall fescue, while drought priming had no significant effects on heat tolerance in ABA-deficient Arabidopsis plants. Application of fluridone to tall fescue and ABA-deficient mutants of Arabidopsis exhibited diminished or attenuated positive effects of drought priming on heat tolerance. ABA mediation of acquired heat tolerance by drought priming was associated with the upregulation of CDPK3, MPK3, DREB2A, AREB3, MYB2, MYC4, HsfA2, HSP18, and HSP70. Our study revealed the roles of ABA in drought priming-enhanced heat tolerance, which may involve transcriptional regulation for stress signaling, ABA responses and heat protection.

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“…It has been established that ABA induces thermotolerance in wheat (Hu et al ) and rice (Zhang et al ). Also, heat‐induced damage is more severe in ABA‐deficient mutants than in their parental cultivars (Wang et al , Wu et al ). In addition, fluridone, an inhibitor of phytoene desaturase and hence of carotenoid and ABA biosynthesis, is reported to impair heat tolerance in plants under heat stress by reducing endogenous ABA level (Ding et al , Kumar et al ).…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid prevents pollen abortion under high‐temperature stress by mediating sugar metabolism in rice spikelets

Rezaul

Feng

Chen

et al. 2018

Physiologia Plantarum

107

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Heat stress at the pollen mother cell (PMC) meiotic stage leads to pollen sterility in rice, in which the reactive oxygen species (ROS) and sugar homeostasis are always adversely affected. This damage is reversed by abscisic acid (ABA), but the mechanisms underlying the interactions among the ABA, sugar metabolism, ROS and heat shock proteins in rice spikelets under heat stress are unclear. Two rice genotypes, Zhefu802 (a recurrent parent) and fgl (its near-isogenic line) were subjected to heat stress of 40°C after pre-foliage sprayed with ABA and its biosynthetic inhibitor fluridone at the meiotic stage of PMC. The results revealed that exogenous application of ABA reduced pollen sterility caused by heat stress. This was achieved through various means, including: increased levels of soluble sugars, starch and non-structural carbohydrates, markedly higher relative expression levels of heat shock proteins (HSP24.1 and HSP71.1) and genes related to sugar metabolism and transport, such as sucrose transporters (SUT) genes, sucrose synthase (SUS) genes and invertase (INV) genes as well as increased antioxidant activities and increased content of adenosine triphosphate and endogenous ABA in spikelets. In short, exogenous application of ABA prior to heat stress enhanced sucrose transport and accelerated sucrose metabolism to maintain the carbon balance and energy homeostasis, thus ABA contributed to heat tolerance in rice.

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The Arabidopsis heat‐intolerant 5 (hit5)/enhanced response to aba 1 (era1) mutant reveals the crucial role of protein farnesylation in plant responses to heat stress

Cited by 40 publications

References 64 publications

The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response

The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response

Abscisic acid mediation of drought priming‐enhanced heat tolerance in tall fescue (Festuca arundinacea) and Arabidopsis

Abscisic acid prevents pollen abortion under high‐temperature stress by mediating sugar metabolism in rice spikelets

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