The Arabidopsis histone chaperone FACT is required for stress-induced expression of anthocyanin biosynthetic genes

Pfab, Alexander; Breindl, Matthias; Grasser, Klaus D.

doi:10.1007/s11103-018-0701-5

Cited by 22 publications

(14 citation statements)

References 41 publications

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“…Two genes mapped close to each other on chromosome 2 ( Solyc02g087680 and Solyc02g087690 ) and both coded for members of the FACT subunit complex SSRP1 protein family. It has been reported that, in Arabidopsis, this protein is an epigenetic regulator of seed dormancy and it is also involved in anthocyanin biosynthesis, and in high-light and UV stresses responses [ 42 ]. The third private mutation for E42 mapped in the gene Solyc07g042660 , which codes for an RNA helicase, known to be involved in the responses to different abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Genotyping of Resilient Tomato Landraces to Detect Candidate Genes Involved in the Response to High Temperatures

Olivieri

Calafiore

Francesca

et al. 2020

Genes

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The selection of tolerant varieties is a powerful strategy to ensure highly stable yield under elevated temperatures. In this paper, we report the phenotypic and genotypic characterization of 10 tomato landraces to identify the best performing under high temperatures. The phenotyping of five yield-related traits allowed us to select one genotype that exhibits highly stable yield performances in different environmental conditions. Moreover, a Genotyping-by-Sequencing approach allowed us to explore the genetic variability of the tested genotypes. The high and stable yielding landrace E42 was the most polymorphic one, with ~49% and ~47% private SNPs and InDels, respectively. The effect of 26,113 mutations on proteins’ structure was investigated and it was discovered that 37 had a high impact on the structure of 34 proteins of which some are putatively involved in responses to high temperatures. Additionally, 129 polymorphic sequences aligned against tomato wild species genomes revealed the presence in the genotype E42 of several introgressed regions deriving from S. pimpinellifolium. The position on the tomato map of genes affected by moderate and high impact mutations was also compared with that of known markers/QTLs (Quantitative Trait Loci) associated with reproductive and yield-related traits. The candidate genes/QTLs regulating heat tolerance in the selected landrace E42 could be further investigated to better understand the genetic mechanisms controlling traits for high and stable yield trait under high temperatures.

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Section: Discussionmentioning

confidence: 99%

High-Throughput Genotyping of Resilient Tomato Landraces to Detect Candidate Genes Involved in the Response to High Temperatures

Olivieri

Calafiore

Francesca

et al. 2020

Genes

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“…Recent transcript profiling of 10-day-old Arabidopsis ssrp1 and spt16 seedlings in comparison to the wild type demonstrated that a relatively small subset of genes is differentially expressed in the mutants (Pfab et al, 2018a). The alterations in the transcript profile of both mutants relative to wild type were very similar, consistent with the function of SSRP1 and SPT16 as a heterodimer.…”

Section: Fact In Plant Growth and Developmentmentioning

confidence: 72%

“…Both SSRP1 and SPT16 are nuclear proteins and are ubiquitously expressed in all/most Arabidopsis tissues, but expression is not detectable in certain terminally differentiated cells such as mature trichoblasts or cells of the root cap (Duroux et al, 2004;Ikeda et al, 2011;Pfab et al, 2018a). Consistent with the enrichment of SSRP1 in the highly micrococcal nucleasesensitive fraction of chromatin (Lichota and Grasser, 2001), both SSRP1 and SPT16 are detected by indirect immunofluorescence microscopy in the euchromatin of interphase nuclei, but not in heterochromatic chromocenters (Duroux et al, 2004).…”

Section: Basic Facts About Plant Factmentioning

confidence: 88%

“…Upon exposure to moderate high-light stress several of the anthocyanin biosynthetic genes were induced in the ssrp1/spt16 plants to a lesser extent than in the wild type, and accordingly the mutant plants depleted in FACT accumulated lower amounts of anthocyanin pigments. Expression of SSRP1 and SPT16 was increased under these conditions (Pfab et al, 2018a). Therefore, FACT is required for transcriptional induction leading to anthocyanin accumulation in response to light stress.…”

Section: Fact In Plant Growth and Developmentmentioning

confidence: 92%

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The FACT Histone Chaperone: Tuning Gene Transcription in the Chromatin Context to Modulate Plant Growth and Development

Grasser

2020

Front. Plant Sci.

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FACT is a heterodimeric histone chaperone consisting of the SSRP1 and SPT16 proteins and is conserved among eukaryotes. It interacts with the histones H2A-H2B and H3-H4 as well as with DNA. Based on in vitro and in vivo studies mainly in yeast and mammalian cells, FACT can mediate nucleosome disassembly and reassembly and thus facilitates in the chromatin context DNA-dependent processes including transcription, replication and repair. In plants, primarily the role of FACT related to RNA polymerase II transcription has been examined. FACT was found to associate with elongating Arabidopsis RNA polymerase II (RNAPII) as part of the transcript elongation complex and it was identified as repressor of aberrant intragenic transcriptional initiation. Arabidopsis mutants depleted in FACT subunits exhibit various defects in vegetative and reproductive development. Strikingly, FACT modulates important developmental transitions by promoting expression of key repressors of these processes. Thus, FACT facilitates expression of DOG1 and FLC adjusting the switch from seed dormancy to germination and from vegetative to reproductive development, respectively. In the central cell of the female gametophyte, FACT can facilitate DNA demethylation especially within heterochromatin, and thereby contributes to gene imprinting during Arabidopsis reproduction. This review discusses results particularly from the plant perspective about the contribution of FACT to processes that involve reorganisation of nucleosomes with a main focus on RNAPII transcription and its implications for diverse areas of plant biology.

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“…Coordinated interplay between histone eviction, re-loading and de novo nucleosome assembly is required for the maintenance of genome stability, in which histone chaperones play an essential role (Lankenau et al, 2003; Mozgova et al, 2010; Zhu et al, 2011; Dewari and Bhargava, 2014). In Arabidopsis thaliana , dysfunctions of CHROMATIN ASSEMBLY FACTOR 1 (CAF-1), ANTI-SILENCING FUNCTION 1 (ASF1) or FACILITATING CHROMATIN TRANSCRIPTION (FACT) lead to severe phenotypes, while disruption of HISTONE REGULATOR A (HIRA) or NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) are much better tolerated (Exner et al, 2006; Kirik et al, 2006; Liu et al, 2009; Zhu et al, 2011; Duc et al, 2017; Pfab et al, 2018). This suggests that functions of some histone chaperones can be replaced, and that their mutual interactions represent a survivaldetermining factor.…”

Section: Introductionmentioning

confidence: 99%

Disruption ofNAP1genes supresses thefas1mutant phenotype and enhances genome stability

Kolářová

Loja

et al. 2020

Preprint

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21Histone chaperones mediate assembly and disassembly of nucleosomes and 22 participate in essentially all DNA-dependent cellular processes. In Arabidopsis thaliana, 23 loss-of-functions of FAS1 or FAS2 subunits of the H3-H4 histone chaperone complex 24 CHROMATIN ASSEMBLY FACTOR 1(CAF-1) has a dramatic effect on plant 25 morphology, growth and overall fitness. Altered chromatin compaction, systematic loss 26 of repetitive elements or increased DNA damage clearly demonstrate the severity of 27 CAF-1 dysfunction. How histone chaperone molecular networks change without a 28 functional CAF-1 remains elusive. Here we present an intriguing observation that 29 disruption of the H2A-H2B histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 30 (NAP1) supresses FAS1 loss-of function. The quadruple mutant fas1nap1;1-3 shows 31 wild-type growth and decreased sensitivity to genotoxic stress. Chromatin of fas1nap1;1-32 3 plants is less accessible to micrococcal nuclease and progressive loss of telomeres 33 and 45S rDNA is supressed. Interestingly, the strong genetic interaction between FAS1 34 and NAP1 does not occur via direct protein-protein interaction. We propose that 35 NAP1;1-3 play an essential role in nucleosome assembly in fas1, thus their disruption 36 abolishes fas1 defects. Our data altogether reveal a novel function of NAP1 proteins, 37 unmasked by CAF-1 dysfunction. It emphasizes the importance of a balanced 38 composition of chromatin and shed light on the histone chaperone molecular network. 39 40 42 ability to directly bind histones and mediate their assembly into nucleosomes (Nakagawa 43 et al. , 2001; Takeuchi et al., 2003). This process begins with the interaction of the H3-H4 44 tetramer with DNA (~147 bp), followed by addition of H2A-H2B dimers, which completes 45

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The Arabidopsis histone chaperone FACT is required for stress-induced expression of anthocyanin biosynthetic genes

Cited by 22 publications

References 41 publications

High-Throughput Genotyping of Resilient Tomato Landraces to Detect Candidate Genes Involved in the Response to High Temperatures

High-Throughput Genotyping of Resilient Tomato Landraces to Detect Candidate Genes Involved in the Response to High Temperatures

The FACT Histone Chaperone: Tuning Gene Transcription in the Chromatin Context to Modulate Plant Growth and Development

Disruption ofNAP1genes supresses thefas1mutant phenotype and enhances genome stability

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