The Arabidopsis Cop9 signalosome subunit 4 (CSN4) is involved in adventitious root formation

Păcurar, Daniel Ioan; Păcurar, Monica Lăcrămioara; Lakehal, Abdellah; Pacurar, Andrea; Ranjan, Alok; Bellini, Catherine

doi:10.1038/s41598-017-00744-1

Cited by 20 publications

(21 citation statements)

References 54 publications

(114 reference statements)

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“…Moreover, we demonstrate that WT inflorescence contains more neddylated CUL1, while in WT seedlings non-neddylated CUL1 is more present (Figs 4B and S9B). Inflorescence of a weak mutant of csn4 contains almost exclusively neddylated CUL1 (Fig 4B) in accordance with previous results [28].…”

Section: Resultssupporting

confidence: 92%

TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals

et al. 2019

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Translationally Controlled Tumor Protein (TCTP) controls growth by regulating the G1/S transition during cell cycle progression. Our genetic interaction studies show that TCTP fulfills this role by interacting with CSN4, a subunit of the COP9 Signalosome complex, known to influence CULLIN-RING ubiquitin ligases activity by controlling CULLIN (CUL) neddylation status. In agreement with these data, downregulation of CSN4 in Arabidopsis and in tobacco cells leads to delayed G1/S transition comparable to that observed when TCTP is downregulated. Loss-of-function of AtTCTP leads to increased fraction of deneddylated CUL1, suggesting that AtTCTP interferes negatively with COP9 function. Similar defects in cell proliferation and CUL1 neddylation status were observed in Drosophila knockdown for dCSN4 or dTCTP, respectively, demonstrating a conserved mechanism between plants and animals. Together, our data show that CSN4 is the missing factor linking TCTP to the control of cell cycle progression and cell proliferation during organ development and open perspectives towards understanding TCTP’s role in organ development and disorders associated with TCTP miss-expression.

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Section: Resultssupporting

confidence: 92%

TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals

et al. 2019

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“…Additional components of the IAA signaling machinery were identified in a genetic screen for suppressors of the AR phenotype of the auxin‐overproducing mutant sur2‐1 (Pacurar et al ). For example, the COP9 SIGNALOSOME SUBUNIT 4 , which controls de‐neddylation of CULLIN1 in the SKP1‐CULLIN‐F‐box (SCF) complex, acts on the ARF6/8 regulatory module and likely at the crossroads of IAA, JA and/or light signaling pathways involved in the control of both AR and LR formation (Pacurar et al ). Intriguingly, phenotypic characterization of available viable csn mutants indicated a possible differential role of the COP9 signalosome in AR and LR formation (Pacurar et al ).…”

Section: Auxin: the Master Player And Hub Of Any Crosstalkmentioning

confidence: 99%

“…For example, the COP9 SIGNALOSOME SUBUNIT 4 , which controls de‐neddylation of CULLIN1 in the SKP1‐CULLIN‐F‐box (SCF) complex, acts on the ARF6/8 regulatory module and likely at the crossroads of IAA, JA and/or light signaling pathways involved in the control of both AR and LR formation (Pacurar et al ). Intriguingly, phenotypic characterization of available viable csn mutants indicated a possible differential role of the COP9 signalosome in AR and LR formation (Pacurar et al ). Moreover, the gain‐of‐function mutants crane‐2 and solitary root‐1, which harbor mutations in domain II of IAA18 and IAA14, respectively, that confer resistance to degradation by the proteasome, were also shown to be affected in ARI (Bustillo‐Avendaño et al ).…”

Section: Auxin: the Master Player And Hub Of Any Crosstalkmentioning

confidence: 99%

Control of adventitious root formation: insights into synergistic and antagonistic hormonal interactions

Lakehal

Bellini

2018

Physiologia Plantarum

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Plants have evolved sophisticated root systems that help them to cope with harsh environmental conditions. They are typically composed of a primary root and lateral roots (LRs), but may also include adventitious roots (ARs). Unlike LRs, ARs may be initiated not only from pericycle cells, but from various cell types and tissues depending on the species. Phytohormones, together with many other internal and external stimuli, coordinate and guide every step of AR formation from the first event of cell reprogramming until emergence and outgrowth. In this review, we summarize recent advances in the molecular mechanisms controlling AR formation and highlight the main hormonal cross talk involved in its regulation under different conditions and in different model systems.

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“…Effect of Mn and Zn on csn4 and csn7 gene expression in P. nicotianae at the stage of sporangiogenesis COP9 signalosome (CSN) has profound effects on the regulation of the ubiquitin proteasome system, mycelial growth and conidial yield (Pacurar et al, 2017;Licursi et al, 2014;Hind et al, 2011). The filamentous fungus Aspergillus nidulans survives without CSN but is unable to complete sexual development (Nahlik et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The COP9 signaling complex (CSN), an important sensor of lipid oxidation involved in the cellular resistance to oxidative stress, plays a central role in regulating the post-translational processing of fungal proteins as well as in fungal development (Nahlik et al, 2010;Licursi et al, 2014;Pacurar et al, 2017). The fungal CSN is composed of eight subunits, csn1-8 (Hu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Luo

Yao

Tan

et al. 2020

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Background Phytophthora nicotianae is a fungal soil-borne pathogen that damages various plant species. Mancozeb and Zineb, fungicides containing manganese (Mn) and zinc (Zn) as the main components, are widely used to control the diseases caused by Phytophthora. However, the inhibition mechanism is still unclear. The purpose of this study was to examine the effects of Mn and Zn on P. nicotianae and to determine possible inhibitory mechanisms of Mn and Zn on sporangiogenesis of P. nicotianae. Methods The mycelial growth, sporangium generation, zoosporogenesis and zoospore germination of P. nicotianae were observed under Mn and Zn treatments. The gene (csn4 and csn7) expression levels of P. nicotianae in different growth stages were examined. Csn4 and csn7 gene expression, superoxide dismutase (SOD) activity, catalase (CAT) activity and malondialdehyde (MDA) content were tested at the stage of sporangiogenesis under different Mn and Zn concentrations. Results Mycelial growth of P. nicotianae was significantly inhibited by Mn from ≥1 mg/L concentration and by Zn from ≥10 mg/L. The sporangia production, sporangia release, and zoospore germination of P. nicotianae were significantly reduced by Mn at all concentrations, while treatment with Zn from ≥0.5 mg/L concentration significantly inhibited the same processes. At the same concentration, the inhibition rate of Mn on the growth process of P. nicotianae was higher than that of Zn. The csn4 and csn7 gene transcription of P. nicotianae were significantly reduced by all treatments with Mn and Zn at the stage of sporangiogenesis. With the increase of Mn concentration, the activities of SOD and CAT increased to maxima and then decreased, and the content of MDA gradually increased during sporangiogenesis of P. nicotianae. The sporangia production of P. nicotianae was significantly positively correlated with the expression levels of the genes csn4 and csn7. Conclusion The inhibitory effect of Mn on the growth process of P. nicotianae was stronger than that of Zn, especially on sporangiogenesis and zoosporogenesis. A possible mechanism of the inhibitory effect on sporangiogenesis of P. nicotianae was that Mn and Zn acted by inhibiting the expression levels of the genes csn4 and csn7 and by affecting antioxidant enzyme activity (further resulting in lipid peroxidation) in the sporangium of P. nicotianae.

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The Arabidopsis Cop9 signalosome subunit 4 (CSN4) is involved in adventitious root formation

Cited by 20 publications

References 54 publications

TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals

TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals

Control of adventitious root formation: insights into synergistic and antagonistic hormonal interactions

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

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