The Arabidopsis AtbZIP9 protein fused to the VP16 transcriptional activation domain alters leaf and vascular development

Silveira, Amanda Bortolini; Gauer, Luciane; Tomaz, Juarez Pires; Cardoso, Poliana Ramos; Carmello-Guerreiro, Sandra Maria; Vincentz, Michel

doi:10.1016/j.plantsci.2007.03.003

Cited by 49 publications

(28 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They showed a total P value >10% from T000 to T048, perhaps reflecting the requirement for ethylene action in germination. bZIP proteins control seed and leaf development, nitrogen/carbon balance, and photomorphogenesis (33)(34)(35), whereas C3H proteins are implicated in embryogenesis (36) and stress response (37). The NAC gene family maintained high expression (P > 5%) until T030.…”

Section: Expression Dynamics Of Tf Gene Families During Germination Andmentioning

confidence: 99%

Transcriptome dynamics of developing maize leaves and genomewide prediction ofciselements and their cognate transcription factors

Chen²,

Chang³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Maize is a major crop and a model plant for studying C4 photosynthesis and leaf development. However, a genomewide regulatory network of leaf development is not yet available. This knowledge is useful for developing C3 crops to perform C4 photosynthesis for enhanced yields. Here, using 22 transcriptomes of developing maize leaves from dry seeds to 192 h post imbibition, we studied gene up-and down-regulation and functional transition during leaf development and inferred sets of strongly coexpressed genes. More significantly, we developed a method to predict transcription factor binding sites (TFBSs) and their cognate transcription factors (TFs) using genomic sequence and transcriptomic data. The method requires not only evolutionary conservation of candidate TFBSs and sets of strongly coexpressed genes but also that the genes in a gene set share the same Gene Ontology term so that they are involved in the same biological function. In addition, we developed another method to predict maize TF-TFBS pairs using known TF-TFBS pairs in Arabidopsis or rice. From these efforts, we predicted 1,340 novel TFBSs and 253 new TF-TFBS pairs in the maize genome, far exceeding the 30 TF-TFBS pairs currently known in maize. In most cases studied by both methods, the two methods gave similar predictions. In vitro tests of 12 predicted TF-TFBS interactions showed that our methods perform well. Our study has significantly expanded our knowledge on the regulatory network involved in maize leaf development. maize transcriptomes | coexpressed genes | cis binding site M aize (Zea mays) is a major crop and a model plant for studying C4 photosynthesis and leaf development. However, the regulatory network that controls maize leaf development is still not well understood. In fact, the number of known maize transcription factor (TF)-binding sites (TFBSs) is far smaller than that for Arabidopsis thaliana (1-3).To understand better the regulation of maize leaf development, several recent studies used next-generation sequencing (NGS) technologies to survey transcriptomic differences among maize leaf cell types and developmental stages. The first large-scale study was by Li et al. (7) investigated the transcriptomes of Kranz (i.e., the foliar leaf blade) and non-Kranz (the husk leaf sheath) maize leaves to identify cohorts of genes associated with procambium initiation and vascular patterning. Recently, Wang et al. (8) conducted comparative transcriptomic and metabolomic analyses of developing leaves in maize and rice and identified putative structural and regulatory components important for C4 and C3 photosynthesis. These studies provided insights into the regulatory mechanisms underlying the development of Kranz leaf anatomy in maize.In the present study, we obtained nine transcriptomes of the second leaf from 84-192 h post imbibition at 12-h (6:00 AM and 6:00 PM) or 24-h (6:00 PM only) intervals. Together with the 13 SignificanceMaize is a major crop and a model plant for studying C4 leaf development. However, its regulatory network of leaf ...

show abstract

Section: Expression Dynamics Of Tf Gene Families During Germination Andmentioning

confidence: 99%

Transcriptome dynamics of developing maize leaves and genomewide prediction ofciselements and their cognate transcription factors

Chen²,

Chang³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Transgenic lines expressing a translational fusion between the AtbZIP63 promoter and the reporter gene GUS were obtained following the strategy described by Silveira et al (2007). The AtbZIP63 (At5g28770) promoter, 5#UTR sequence (75 bp), and 36 bp coding for the first 12 amino acids (approximately 2.8 kb) were amplified from genomic DNA with two specific primers, 5#-GGTGATTGCCCAATCTGCAGCTTTAATCG-3# and 5#-GTGATGGGATCC-GGAGATTTCTTCG-3#, in which a PstI and a BamHI restriction site, respectively, was introduced to facilitate a translational fusion with GUS in PBI121 plant transformation binary vector (Chen et al, 2003b) from which the cauliflower mosaic virus 35S promoter was removed.…”

Section: Dna Constructs Plant Transformation and Gus Detectionmentioning

confidence: 99%

“…Two homozygous lines (PH3.13.4 and PH3.35.8) for a single transgenic locus were selected based on kanamycin resistance segregation, and the presence of the chimeric gene was verified by PCR amplification. In situ detection of GUS activity was realized as described by Silveira et al (2007). Fluorometric GUS assays were performed as described previously (Jefferson et al, 1987).…”

Section: Dna Constructs Plant Transformation and Gus Detectionmentioning

confidence: 99%

“…cDNA synthesis from 1.5 mg of total RNA (final volume of 12.5 mL) was performed using ImProm II Reverse Transcriptase (Promega) and oligo(dT) 18 essentially according to the manufacturer's instructions. Semiquantitative PCR conditions were basically realized as described by Silveira et al (2007). Control genes for ABA, Glc, and Man treatments were Rd29a for ABA (At5g52310; Arroyo et al, 2003) and XTR7 for Glc and Man (At4g14130; Price et al, 2004), respectively.…”

Section: Dna Constructs Plant Transformation and Gus Detectionmentioning

confidence: 99%

See 1 more Smart Citation

The Arabidopsis bZIP Gene AtbZIP63 Is a Sensitive Integrator of Transient Abscisic Acid and Glucose Signals

et al. 2011

Self Cite

View full text Add to dashboard Cite

Glucose modulates plant metabolism, growth, and development. In Arabidopsis (Arabidopsis thaliana), Hexokinase1 (HXK1) is a glucose sensor that may trigger abscisic acid (ABA) synthesis and sensitivity to mediate glucose-induced inhibition of seedling development. Here, we show that the intensity of short-term responses to glucose can vary with ABA activity. We report that the transient (2 h/4 h) repression by 2% glucose of AtbZIP63, a gene encoding a basic-leucine zipper (bZIP) transcription factor partially involved in the Snf1-related kinase KIN10-induced responses to energy limitation, is independent of HXK1 and is not mediated by changes in ABA levels. However, high-concentration (6%) glucose-mediated repression appears to be modulated by ABA, since full repression of AtbZIP63 requires a functional ABA biosynthetic pathway. Furthermore, the combination of glucose and ABA was able to trigger a synergistic repression of AtbZIP63 and its homologue AtbZIP3, revealing a shared regulatory feature consisting of the modulation of glucose sensitivity by ABA. The synergistic regulation of AtbZIP63 was not reproduced by an AtbZIP63 promoter-5#-untranslated region::b-glucuronidase fusion, thus suggesting possible posttranscriptional control. A transcriptional inhibition assay with cordycepin provided further evidence for the regulation of mRNA decay in response to glucose plus ABA. Overall, these results indicate that AtbZIP63 is an important node of the glucose-ABA interaction network. The mechanisms by which AtbZIP63 may participate in the finetuning of ABA-mediated abiotic stress responses according to sugar availability (i.e., energy status) are discussed.

show abstract

“…For example, the Arabidopsis thaliana genome contains 75 bZIP genes (Jakoby et al 2002), both 89 and 92 have been reported to be present in rice (Oryza sativa; Nijhawan et al 2008;Correa et al 2008), 47 have been identified in soybean (Glycine max; Liao et al 2008), 92 in sorghum (Sorghum bicolor; Wang et al 2011), 125 in maize (Zea mays; Wei et al 2012), 47 (Gao et al 2014) and 55 in grapevine (Vitis vinifera). Plant bZIP TFs are involved in regulating many metabolic processes, such as energy metabolism (Baena-González et al 2007), cell elongation (Fukazawa et al 2000), organ and tissue differentiation (Silveira et al 2007), embryogenesis (Guan et al 2009), floral induction and development (Abe et al 2005;Zou et al 2008), seed maturation (Jakoby et al 2002;Cheng et al 2014), plant senescence (Smykowski et al 2010), hormone signaling (Fujita et al 2005;Kang et al 2002;Kim et al 2004), photomorphogenesis (Huang et al 2012), and light signaling (Mallappa et al 2006). In addition, they control responses to a variety of abiotic stimuli, such as high salinity (Hsieh et al 2010;García et al 2012), drought (Yoshida et al 2010), cold stress ) and heat stress .…”

Section: Introductionmentioning

confidence: 99%

Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses

Wang

Huang

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The basic region/leucine zipper (bZIP) transcription factors are known to play key roles in response to abiotic stress. In this study, a bZIP gene (VqbZIP39) was isolated from grape (Vitis quinquangularis) and constitutively expressed in Arabidopsis under control of the cauliflower mosaic virus 35S promoter. The transgenic Arabidopsis thaliana plants showed enhance salt and drought stress tolerance during seed germination and in the seedling and mature plant stages. Various physiological parameters related to stress responses were analyzed to gain further insight into the role of VqbZIP39 and it was found that osmotic stress caused less damage to the transgenic seedlings than to the corresponding wild type plants. This correlated with an increase in endogenous ABA content as a consequence of the constitutive overexpression of VqbZIP39, and the up-regulated expression of stress-inducible target genes associated with tolerance of drought, high-salt, and oxidative stresses. Our results suggest that the expression of VqbZIP39 in A. thaliana likely enhances the tolerance to multiple abiotic stresses through the ABA signaling pathway, and may therefore have a similar function in the response to abiotic stresses in grape.

show abstract

The Arabidopsis AtbZIP9 protein fused to the VP16 transcriptional activation domain alters leaf and vascular development

Cited by 49 publications

References 28 publications

Transcriptome dynamics of developing maize leaves and genomewide prediction ofciselements and their cognate transcription factors

Transcriptome dynamics of developing maize leaves and genomewide prediction ofciselements and their cognate transcription factors

The Arabidopsis bZIP Gene AtbZIP63 Is a Sensitive Integrator of Transient Abscisic Acid and Glucose Signals

Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses

Contact Info

Product

Resources

About