The Arabidopsis At1g45130 and At3g52840 genes encode β-galactosidases with activity toward cell wall polysaccharides

Gantulga, Dashzeveg; Turan, Yusuf; Bevan, David R.; Esen, Asım

doi:10.1016/j.phytochem.2008.01.023

Cited by 33 publications

(21 citation statements)

References 31 publications

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“…Currently, no study has yet explored the biochemical function of sub-family B BGALs. Expression data have revealed that AtBGAL7 and AtBGAL15 , arabidopsis members of subfamily B, are expressed in flowers and pollen [23,51], whereas AtBGAL18 is expressed in seedlings and roots [51]. Similar to AtBGAL7 and AtBGAL15 , OsBGALs 5, 12, 14, and 15, the rice representatives of sub-family B, have also shown enrichment in reproductive tissues, which led to the hypothesis that the ancestor to sub-family B developed a reproductive-tissue specific role antecedent to the divergence of monocots and dicots [24].…”

Section: Discussionmentioning

confidence: 99%

Genomic and expression analysis of the flax (Linum usitatissimum) family of glycosyl hydrolase 35 genes

Hobson

Deyholos

2013

BMC Genomics

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BackgroundSeveral β-galactosidases of the Glycosyl Hydrolase 35 (GH35) family have been characterized, and many of these modify cell wall components, including pectins, xyloglucans, and arabinogalactan proteins. The phloem fibres of flax (Linum usitatissimum) have gelatinous-type cell walls that are rich in crystalline cellulose and depend on β-galactosidase activity for their normal development. In this study, we investigate the transcript expression patterns and inferred evolutionary relationships of the complete set of flax GH35 genes, to better understand the functions of these genes in flax and other species.ResultsUsing the recently published flax genome assembly, we identified 43 β-galactosidase-like (BGAL) genes, based on the presence of a GH35 domain. Phylogenetic analyses of their protein sequences clustered them into eight sub-families. Sub-family B, whose members in other species were known to be expressed in developing flowers and pollen, was greatly under represented in flax (p-value < 0.01). Sub-family A5, whose sole member from arabidopsis has been described as its primary xyloglucan BGAL, was greatly expanded in flax (p-value < 0.01). A number of flax BGALs were also observed to contain non-consensus GH35 active sites. Expression patterns of the flax BGALs were investigated using qRT-PCR and publicly available microarray data. All predicted flax BGALs showed evidence of expression in at least one tissue.ConclusionFlax has a large number of BGAL genes, which display a distinct distribution among the BGAL sub-families, in comparison to other closely related species with available whole genome assemblies. Almost every flax BGAL was expressed in fibres, the majority of which expressed predominately in fibres as compared to other tissues, suggesting an important role for the expansion of this gene family in the development of this species as a fibre crop. Variations displayed in the canonical GH35 active site suggest a variety of roles unique to flax, which will require further characterization.

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Section: Discussionmentioning

confidence: 99%

Genomic and expression analysis of the flax (Linum usitatissimum) family of glycosyl hydrolase 35 genes

Hobson

Deyholos

2013

BMC Genomics

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“…Cell wall localization of BGAL6 has been observed using a fusion with GFP, transiently expressed in Nicotiana tabacum leaves [31]. BGAL2 and BGAL5 have been detected in cell wall fractions of Arabidopsis leaves by dotblotting [33], whereas BGAL8 has been detected in the cell walls of Arabidopsis stems by proteomics [34]. In conclusion, all six characterized BGALs in Arabidopsis to date are localized in the cell wall, implicating their role in cell wall remodelling and expansion ( Figure 1D).…”

Section: All Tested Arabidopsis Bgals Were Detected In the Cell Wallmentioning

confidence: 81%

Beta galactosidases in Arabidopsis and tomato–a mini review

Chandrasekar

Hoorn

2016

Biochemical Society Transactions

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Beta galactosidases (BGALs) are glycosyl hydrolases that remove terminal β-D-galactosyl residues from β-D-galactosides. There are 17 predicted BGAL genes in the genomes of both Arabidopsis (BGAL1-17) and tomato (TBG1-17). All tested BGALs have BGAL activity but their distinct expression profiles and ancient phylogenetic separation indicates that these enzymes fulfil diverse, non-redundant roles in plant biology. The majority of these BGALs are predicted to have signal peptide and thought to act during cell wall-related biological processes. Interestingly, deletion of BGAL6 and BGAL10 in Arabidopsis causes reduced mucilage release during seed imbibition and shorter siliques respectively, whereas TBG4 depletion by RNAi decreases in fruit softening in tomato. The majority of plant BGALs remain to be characterized.

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“…These associations were not identified in A. thaliana, but in β-galactosidase metabolism, the contig is related to an enzyme essential to cell expansion and signaling of biochemical events in cells during plant development (Pauly et al, 2001). Gantulga et al (2008) have isolated and characterized the genes At1g45130 and At3g52840, which encode Gal-5 and Gal-2 β-galactosidases that belong to the 35-glycosyl hydrolase family, which is involved in the modification of polysaccharides from the cell wall and expressed mainly in leaves, stems, and flowers.…”

Section: Resultsmentioning

confidence: 99%

Genes expressed in cotton (Gossypium hirsutum) buds isolated with a subtractive library

Pinheiro

Batista²,

Martins³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.A subtractive cDNA library from cotton buds was constructed to prospect for differentially expressed genes related to early bud development. A library was constructed and 768 cDNA sequences were obtained, comprising 168 clusters, with 126 contigs and 42 singlets. Both the Gossypium as well as Arabidopsis databases were utilized for the in silico analysis, since some genes identified in cotton have not yet been studied for functionality, although they have homology with genes from other species. The transcriptome revealed a large number of transcripts, some of them with unknown function, and others related to pollen development, pollen tubes, ovules, and fibers at different stages. The most populated contig was identified as fiber from 0-10 days after anthesis, with 12 reads. The success and novelty rates generated from the library were 67 and 51%, respectively. The information obtained here will provide a framework for research on functional cotton genomics.

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The Arabidopsis At1g45130 and At3g52840 genes encode β-galactosidases with activity toward cell wall polysaccharides

Cited by 33 publications

References 31 publications

Genomic and expression analysis of the flax (Linum usitatissimum) family of glycosyl hydrolase 35 genes

Genomic and expression analysis of the flax (Linum usitatissimum) family of glycosyl hydrolase 35 genes

Beta galactosidases in Arabidopsis and tomato–a mini review

Genes expressed in cotton (Gossypium hirsutum) buds isolated with a subtractive library

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