The Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development.

Rodrigues-Pousada, Renato A.; Rycke, Riet De; Dedonder, A.; Caeneghem, Wim Van; Engler, Gilbert; Montagu, Marc Van; Straeten, Dominique Van Der

doi:10.1105/tpc.5.8.897

Cited by 120 publications

(66 citation statements)

References 73 publications

(118 reference statements)

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“…Previously, ethylene biosynthesis has been shown to be several times higher in seedlings and in young plants than in mature plants ( Rodrigues-Pousada et al, 1993;Bürstenbinder et al, 2007). Consistent with this, expression of ACS1 was shown to be high in young tissues and switched off in mature tissues (Rodrigues-Pousada et al, 1993).…”

Section: Discussionsupporting

confidence: 56%

“…Consistent with this, expression of ACS1 was shown to be high in young tissues and switched off in mature tissues (Rodrigues-Pousada et al, 1993). In this study, 1-Aminocyclopropanecarboxylate (ACC) could not be detected in inflorescences of both mutant and wildtype plants with neither ultraperformance liquid chromatography (UPLC) nor ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) measurements.…”

Section: Discussionsupporting

confidence: 49%

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Phloem-Specific Methionine Recycling Fuels Polyamine Biosynthesis in a Sulfur-Dependent Manner and Promotes Flower and Seed Development

et al. 2015

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The Yang or Met Cycle is a series of reactions catalyzing the recycling of the sulfur (S) compound 59-methylthioadenosine (MTA) to Met. MTA is produced as a by-product in ethylene, nicotianamine, and polyamine biosynthesis. Whether the Met Cycle preferentially fuels one of these pathways in a S-dependent manner remained unclear so far. We analyzed Arabidopsis (Arabidopsis thaliana) mutants with defects in the Met Cycle enzymes 5-METHYLTHIORIBOSE-1-PHOSPHATE-ISOMERASE1 (MTI1) and DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1 (DEP1) under different S conditions and assayed the contribution of the Met Cycle to the regeneration of S for these pathways. Neither mti1 nor dep1 mutants could recycle MTA but showed S-dependent reproductive failure, which was accompanied by reduced levels of the polyamines putrescine, spermidine, and spermine in mutant inflorescences. Complementation experiments with external application of these three polyamines showed that only the triamine spermine could specifically rescue the S-dependent reproductive defects of the mutant plants. Furthermore, expressing gene-reporter fusions in Arabidopsis showed that MTI1 and DEP1 were mainly expressed in the vasculature of all plant parts. Phloem-specific reconstitution of Met Cycle activity in mti1 and dep1 mutant plants was sufficient to rescue their S-dependent mutant phenotypes. We conclude from these analyses that phloem-specific S recycling during periods of S starvation is essential for the biosynthesis of polyamines required for flowering and seed development.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionsupporting

confidence: 49%

Phloem-Specific Methionine Recycling Fuels Polyamine Biosynthesis in a Sulfur-Dependent Manner and Promotes Flower and Seed Development

et al. 2015

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“…Many of the ACS genes are regulated on the transcriptional level. ACS2 transcription in leaves is switched off when tissues mature (Rodrigues-Pousada et al, 1993; in this paper the gene was designated ACS1). ACS4 can be induced by auxins (Abel et al, 1995).…”

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confidence: 99%

Circadian Rhythms of Ethylene Emission in Arabidopsis

et al. 2004

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Ethylene controls multiple physiological processes in plants, including cell elongation. Consequently, ethylene synthesis is regulated by internal and external signals. We show that a light-entrained circadian clock regulates ethylene release from unstressed, wild-type Arabidopsis (Arabidopsis thaliana) seedlings, with a peak in the mid-subjective day. The circadian clock drives the expression of multiple ACC SYNTHASE genes, resulting in peak RNA levels at the phase of maximal ethylene synthesis. Ethylene production levels are tightly correlated with ACC SYNTHASE 8 steady-state transcript levels. The expression of this gene is controlled by light, by the circadian clock, and by negative feedback regulation through ethylene signaling. In addition, ethylene production is controlled by the TIMING OF CAB EXPRESSION 1 and CIRCADIAN CLOCK ASSOCIATED 1 genes, which are critical for all circadian rhythms yet tested in Arabidopsis. Mutation of ethylene signaling pathways did not alter the phase or period of circadian rhythms. Mutants with altered ethylene production or signaling also retained normal rhythmicity of leaf movement. We conclude that circadian rhythms of ethylene production are not critical for rhythmic growth.Since the discovery of ethylene production in plants in the 1930s, researchers have tried to elucidate mechanisms governing ethylene formation. A major breakthrough was the completion of the enzymatic pathway for ethylene biosynthesis 50 years later (for review, see Yang and Hoffman, 1984). Shortly thereafter, the first genes encoding ethylene biosynthetic enzymes were cloned (Sato and Theologis, 1989;Van Der Straeten et al., 1990;Hamilton et al., 1991;Spanu et al., 1991). With the use of tomato (Lycopersicon esculentum) and especially Arabidopsis (Arabidopsis thaliana) as model plants, molecular biological and genetic analysis has shed light on many physiological processes involving ethylene (Abeles et al., 1992;Somerville and Meyerowitz, 2002). In higher plants, the enzymes for ethylene biosynthesis are encoded by gene families. The members of these families are differentially responsive to various ethylene-inducing factors, including wounding, fruit ripening, pathogen infections, auxins, and cytokinins (for review, see Fluhr and Mattoo, 1996).In Arabidopsis, there are 12 genes in the family of enzymes that produces the ethylene precursor 1-amino-cyclopropane-1-carboxylic acid (ACC), one of which, ACC SYNTHASE 3 (ACS3), is a pseudogene (Yamagami et al., 2003;Tsuchisaka and Theologis, 2004). ACS1 is not functional as an ACS (Liang et al., 1992). ACS10 and ACS12 do not function as ACSs either, but as aminotransferases (Yamagami et al., 2003). Many of the ACS genes are regulated on the transcriptional level. ACS2 transcription in leaves is switched off when tissues mature (Rodrigues-Pousada et al., 1993; in this paper the gene was designated ACS1). ACS4 can be induced by auxins (Abel et al., 1995). ACS5 is regulated by cytokinins that cause stabilization of the protein (Chae et al., 2003). ACS6 is induce...

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“…The expression of AtACS1 is under developmental control both in shoot and root. High expression was observed in young tissues and was switched off in mature tissues (Rodrigues-Pousada et al, 1993). However, little is known so far about the expression of pear ACS genes during early development.…”

mentioning

confidence: 99%

“…Lots of important ACC synthase genes have been isolated and characterized from the plant kingdom. For examples, ACS genes were identified in pear (Pyrus communis) (El-Sharkawy et al, 2004), Japanese pear (Pyrus pyrifolia) (Itai et al, 1999), apple (Rosenfield et al, 1996;Wang et al, 2009), peach (Mathooko et al, 2001;Tatsuki et al, 2006), pineapple (Cazzonelli et al, 1998;Botella et al, 2000;Trusov et al, 2006), plum (El-Sharkawy et al, 2008), cucumber (Mathooko et al, 1999), watermelon (Salman-Minkov et al, 2008), Arabidopsis (Rodrigues-Pousada et al, 1993;Mayer et al, 1999) , tomato (Nakatsuka et al, 1997), etc.…”

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confidence: 99%

Molecular Characterization of Pear 1-Aminocyclopropane-1-Carboxylate Synthase Gene Preferentially Expressed in Leaves

Shi¹,

Zhang²,

Sun³

et al. 2012

JAS

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Abstract1-Aminocyclopropane-1-carboxylate (ACC) synthase catalyzes the conversion of S-adenosyl-L-methionine to ACC in the ethylene biosynthetic pathway. Lots of important ACC synthase genes have been isolated and characterized from the plant kingdom. In this study, a cDNA clone encoding putative ACC synthase was isolated from a cDNA library produced using mRNA from pear (Pyrus pyrifolia). The cDNA clone, designated PpACS1 (GenBank accession No. JQ284383), comprised an open reading frame of 1, 341 bp encoding a protein of 446 amino acids that shares high similarity with the known plant ACSs. Using PCR amplification techniques, a genomic clone corresponding to PpACS1 was isolated and shown to contain two introns with typical GT/AG boundaries defining the splice junctions. The PpACS1 gene product shared 97% identity with an ACC synthase from pear (Pyrus communis), and phylogenetic analyses clearly placed the gene product in the ACC synthase cluster of the plant ACS superfamily tree. RT-PCR analysis indicated that the PpACS1 gene was preferentially expressed in pear leaves. The transcript of PpACS1 gene was accumulated at relatively high levels in anthers. The expression signal was detected in shoot at relatively low levels, but none signal was detected in developing fruit of pear. These results suggested that the PpACS1 may participate in the regulation of ethylene production in pear leaves and anthers.

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The Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development.

Cited by 120 publications

References 73 publications

Phloem-Specific Methionine Recycling Fuels Polyamine Biosynthesis in a Sulfur-Dependent Manner and Promotes Flower and Seed Development

Phloem-Specific Methionine Recycling Fuels Polyamine Biosynthesis in a Sulfur-Dependent Manner and Promotes Flower and Seed Development

Circadian Rhythms of Ethylene Emission in Arabidopsis

Molecular Characterization of Pear 1-Aminocyclopropane-1-Carboxylate Synthase Gene Preferentially Expressed in Leaves

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