The Aquaporin 3 Promoter Polymorphism −1431 A/G is Associated with Acute Graft Rejection and Cytomegalovirus Infection in Kidney Recipients Due to Altered Immune Cell Migration

Rump, Katharina; Rahmel, Tim; Rustige, Anna-Maria; Unterberg, Matthias; Nowak, Hartmuth; Koos, Björn; Schenker, Peter; Viebahn, Richard; Adamzik, Michael; Bergmann, Lars

doi:10.3390/cells9061421

Cited by 6 publications

(5 citation statements)

References 48 publications

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“…However, we observed a significantly higher incidence of CMV infection among middle-aged patients (31–50 years). Our results concerning gender agreed with previous studies; however, several studies have shown no association between age and the risk of CMV infection among the post-renal transplant recipients [ 1 , 7 , 8 ]. Our finding could be attributed to the higher proportion of middle-aged individuals in our sample pool.…”

Section: Discussionsupporting

confidence: 92%

“…Several studies have reported no significant correlation between preoperative diabetes and risk of CMV infection [ 7 , 8 ]. Another study conducted by Beyler et al also showed a limited association between preoperative diabetes and postoperative CMV incidence; however, they attributed their results to a small number of patients and a short follow-up period [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

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Cytomegalovirus infection after renal transplantation

Atbee

Tuama

2022

JMedLife

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Renal transplant patients show a high prevalence of cytomegalovirus (CMV) infection after the procedure. This study was conducted to assess the prevalence and factors associated with the incidence of CMV infection among renal transplant patients. A total of 100 patients were recruited in this study. The CMV load in the blood of each patient was assessed using the technique of polymerase chain reaction (PCR). The serostatus of all recipients and donors was examined preoperatively and those of the recipients again postoperatively. The association of CMV load was assessed with the following factors: age, gender, alanine aminotransferase (ALT) and serum creatinine levels, types of immunosuppressive and induction regimens, preoperative diabetes status, and serological virologic response (SVR) at 12 weeks postoperatively. Our findings showed that CMV incidence was significantly higher in middle-aged patients (62 of 66 patients, 93.9%; p=0.0001). Furthermore, about 88.2% of patients induced by anti-thymocyte globulin (ATG) showed a high viral load, significantly higher than the proportion of CMV-positive patients induced by basiliximab (p=0.001). In addition, a higher proportion of CMV-negative recipients who received the graft from CMV-positive donors and vice-versa were CMV-positive postoperatively. Administration of Valcyte 450 showed 100% efficiency in decreasing the CMV load in the patients. Among all the assessed factors, only the age of the recipients, type of induction therapy used, and the preoperative serostatus of both donors and recipients were significantly associated with the postoperative CMV incidence among the patients.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Cytomegalovirus infection after renal transplantation

Atbee

Tuama

2022

JMedLife

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“…Here, AQP3 regulates T-cell migration by mediating the transport of hydrogen peroxide [18]. As we have also demonstrated in the past, a polymorphism in the AQP3 gene is associated with T-cell migration [19], and altered T-cell migration could play a role in sepsis patients. Furthermore, AQP3 mediates both chemokine production and T cell trafficking [20].…”

Section: Introductionsupporting

confidence: 53%

“…AQP3 is involved in T-cell function, and a different AQP3 expression alters T-cell migration [19,30]. Hence, in our study, an altered T-cell function could impact sepsis survival.…”

Section: Discussionmentioning

confidence: 63%

The Aquaporin 3 Polymorphism (rs17553719) Is Associated with Sepsis Survival and Correlated with IL-33 Secretion

Ziehe,

Marko,

Thon

et al. 2024

IJMS

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Sepsis is a common life-threatening disease caused by dysregulated immune response and metabolic acidosis which lead to organ failure. An abnormal expression of aquaporins plays an important role in organ failure. Additionally, genetic variants in aquaporins impact on the outcome in sepsis. Thus, we investigated the polymorphism (rs17553719) and expression of aquaporin-3 (AQP3) and correlated these measurements with the survival of sepsis patients. Accordingly, we collected blood samples on several days (plus clinical data) from 265 sepsis patients who stayed in different ICUs in Germany. Serum plasma, DNA, and RNA were then separated to detect the promotor genotypes of AQP3 mRNA expression of AQP3 and several cytokines. The results showed that the homozygote CC genotype exhibited a significant decrease in 30-day survival (38.9%) compared to the CT (66.15%) and TT genotypes (76.3%) (p = 0.003). Moreover, AQP3 mRNA expression was significantly higher and nearly doubled in the CC compared to the CT (p = 0.0044) and TT genotypes (p = 0.018) on the day of study inclusion. This was accompanied by an increased IL-33 concentration in the CC genotype (day 0: p = 0.0026 and day 3: p = 0.008). In summary, the C allele of the AQP3 polymorphism (rs17553719) shows an association with increased AQP3 expression and IL-33 concentration accompanied by decreased survival in patients with sepsis.

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“…Clinical manifestations can range from mild to severe life-threatening diseases [ 4 ] and can present as a non-specific systemic viral disease [ 5 ] or a localized tissue-invasive infection, most commonly including pneumonia [ 6 ], hepatitis [ 7 ], colitis [ 8 ], and retinitis [ 9 , 10 ]. The diagnostic of human cytomegalovirus plays an important role in the follow-up and treatment of transplant patients because it is a cause of graft rejection [ 11 ]. Different studies report the diagnostic methods of human cytomegalovirus, such as the serological methods that determine the presence of IgG antibodies, which is an indicator of infection in the past and of IgM for recent infection [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comparability of CMV DNA Extraction Methods and Validation of Viral Load

Uwiringiyeyezu

Khalfi

Saı̈le

et al. 2022

MPs

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Human cytomegalovirus is a herpesvirus that has a worldwide seroprevalence of more than 60% of adults in developed countries and 90% in developing countries. Severe disabilities in newborns are characteristic of the human cytomegalovirus congenital infection, and this virus is implicated in graft rejection in transplant patients. To treat and follow-up the infection, the CMVPCR viral loads are required, and the DNA extraction step remains very important; however, the quantity, quality, and purity of extracted DNA from different biological fluids influence the results of PCR amplification, that is why for reliable results, the choice of nucleic acid extraction methods requires careful attention. Materials and methods: In this study, we compare 4 protocols, I (EZ1 DSP Virus kit), II (EZ1 Virus mini kit), III (QIAamp DSP virus kit), and IV (heating); the extractions are made from plasma collected on EDTA tubes, and the concentration of extracted DNA was measured on NanoDrop Lite followed by real-time CMVPCR using an Artus CMV QS-RGQ kit. All protocols are performed following the manufacturer’s instructions. Results: This study is conducted on the samples of 135 transplant patients whose follow-up medical tests related to human cytomegalovirus infection; since most of the CMVPCR results are negative, we have chosen the 10 CMVPCR positive samples and 2 negative samples as controls to conduct this comparison study. By using NanoDrop Lite to evaluate the DNA concentration, the yield of extracted DNA is higher in our heating protocol than other protocols, the EZ1 DSP virus kit and EZ1 Virus mini kit show homogeneous quantities, and the QIAamp DSP virus kit shows very low DNA yields. Comparing cycle threshold and viral loads by real-time PCR, all these protocols identified negative samples (100%), and the previously positive samples used were as follows: protocol IV (90%), protocol II (60%), and protocol I (40%). QIAamp DSP virus kit results were not real-time PCR applicable and were non-conclusive because of the low DNA yields. Conclusion: Our developed heating method (protocol IV) is very effective, reliable, simple, fast, and cheap compared to the other protocols in our study.

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The Aquaporin 3 Promoter Polymorphism −1431 A/G is Associated with Acute Graft Rejection and Cytomegalovirus Infection in Kidney Recipients Due to Altered Immune Cell Migration

Cited by 6 publications

References 48 publications

Cytomegalovirus infection after renal transplantation

Cytomegalovirus infection after renal transplantation

The Aquaporin 3 Polymorphism (rs17553719) Is Associated with Sepsis Survival and Correlated with IL-33 Secretion

Comparability of CMV DNA Extraction Methods and Validation of Viral Load

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