The Applied Use of Next Generation DNA Sequencing for Infectious Disease

Fry, Stephen E.

doi:10.18103/mra.v5i11.1645

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…The prominent fungi in the sample Funneliformis mossea (formerly Glomus mosseae) is a common arbuscular mycorrhizal soil fungus, uncultivable, with worldwide distribution. It has been reported in humans by our laboratory [5][6][7]. The use of Doxycycline augments the fungal activity of terbinafine; doxycycline may play a role in reducing the fungal biofilm allowing the antifungal terbinafine to work more Austin J Clin Case Rep 8(9): id1229 (2021) -Page -02…”

Section: Discussionmentioning

confidence: 96%

Next Generation DNA Sequencing Diagnoses Fungal Etiology in Chronic Respiratory Disease, Clinical Response Supports Clinical Validity

Gordon¹,

SE²

2021

austinjclincaserep

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A 71-year white male presented to pulmonologists with progressively worsening respiratory problems with a chronic productive cough, asthma symptoms and malaise. The patient was subjected to the traditional treatment of antibiotics, cough suppressants, and inhaled bronchodilator and corticosteroids. After multiple failed combinations of antibiotics, cough suppressants, and inhalers, the patient had a sputum sample, which was analyzed by special stains and targeted Next Generation DNA Sequencing (NGS). Fungal eukaryotic (18S) NGS identified by ‘best match’, Candidae parapsilosis and C. viswanathii and an organism in the Glomerulaceae family, Funnelformis mosseae. The patient was prescribed an antifungal in tandem with an antibiotic, Terbinafine and Doxycycline. The patient rapidly improved to his baseline. This communication communicates the potential of NGS microbial sequencing in pathogen identification in chronic pulmonary disease and improved clinical outcome.

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Section: Discussionmentioning

confidence: 96%