The Application of the Biolog EcoPlate Approach in Ecotoxicological Evaluation of Dairy Sewage Sludge

Gryta, Agata; Frąc, Magdalena; Oszust, Karolina

doi:10.1007/s12010-014-1131-8

Cited by 174 publications

(99 citation statements)

References 33 publications

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“…Absorbance readings were taken periodically (after 24, 48, and 144 h) at 590 nm with a microplate reader (Synergy 2, BioTek, USA). The metabolic response (MR) of consortium was expressed as average well optical density of all carbon sources (MR=Σ ODi/31; Gryta et al 2014).…”

Section: The Impact Of Heavy Metals On Metabolic Activity Of Consortiummentioning

confidence: 99%

Zinc bioaccumulation by microbial consortium isolated from nickel smelter sludge disposal site

Kvasnová¹,

Hamarová²,

Pristaš³

2017

Nova Biotechnologica Et Chimica

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Heavy metal pollution is one of the most important environmental issues of today. Bioremediation by microorganisms is one of technologies extensively used for pollution treatment. In this study, we investigated the heavy metal resistance and zinc bioaccumulation by microbial consortium isolated from nickel sludge disposal site near Sereď (Slovakia). The composition of consortium was analyzed based on MALDI-TOF MS of cultivable bacteria and we have shown that the consortium was dominated by bacteria of genus Arthrobacter. While consortium showed very good growth in the zinc presence, it was able to remove only 15 % of zinc from liquid media. Selected members of consortia have shown lower growth rates in the zinc presence but selected isolates have shown much higher bioaccumulation abilities compared to whole consortium (up to 90 % of zinc removal for NH1 strain). Bioremediation is frequently accelerated through injection of native microbiota into a contaminated area. Based on data obtained in this study, we can conclude that careful selection of native microbiota could lead to the identification of bacteria with increased bioaccumulation abilities.

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Section: The Impact Of Heavy Metals On Metabolic Activity Of Consortiummentioning

confidence: 99%

Zinc bioaccumulation by microbial consortium isolated from nickel smelter sludge disposal site

Kvasnová¹,

Hamarová²,

Pristaš³

2017

Nova Biotechnologica Et Chimica

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“…ETLaNDS, which account for 5~8% of the earth's surface, offer a high degree of biodiversity, and have important ecological functions such as water purification and flood control [1][2][3]. Moreover, wetland ecosystems play key roles in regulating global greenhouse gases and carbon sinks.…”

mentioning

confidence: 99%

Effects of Different Land Use on Soil Bacterial Functional Diversity in Sanjiang Plain, Northeast China

Wang¹,

Sui²,

Zhang³

et al. 2017

JRST

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ABSTRACT:To investigate the bacterial functional diversity regarding soil carbon metabolism in Sanjiang Plain wetlands, the top soils (0-20 cm) of three different land use types were collected in Sanjiang Plain, China. The distribution and variation of carbon source utilization was assessed by the Biolog microplate method. The results showed that the physicochemical properties of the soil were significantly different between the three land use types, wetland, farmland and forest (P < 0.05). The Average Well Color Development (AWCD) of all samples increased with incubation time, whereby the values of AWCD were highest in soil samples from wetland and lowest from forest. The soil bacterial diversity differed significantly between the three different soil types, and the McIntosh and Shannon-Wiener diversity index was significantly lower for forest soil. This shows that the bacterial community structures were influenced by soil physicochemical properties, with the AWCD and diversity index decreasing from wetlands to farmlands to forests. The utilization of six categories of carbon sources by soil bacteria showed significant differences for carbohydrates, polymers and carboxylic acids, but not for phenolic acids, amino acids and amines (P > 0.05). Principal component analysis (PCA) indicated that soil bacterial carbon utilization revealed obvious spatial variation, suggesting the bacterial metabolism diversity could be reflected in the use of carbohydrates, amino acids and carboxylic acids, particularly carbohydrates. In addition, soil physicochemical properties and vegetation composition were important factors modifying soil bacterial composition and functional activity in different land use types.

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“…This methodology compares the use of different carbon sources and is generally used in studies on bacterial communities in various environments such as soil, sludge, fresh water, sediments, biofilms and several other environments (Oliveira et al, 2009;Salomo;Munch;Roske, 2009;Medihala et al, 2012;Gryta;Frac;Oszust, 2014;Patel et al, 2014;Flores-Rentería et al, 2016). By knowing the potential of this method to compare microbial communities in various environments, we opted to use it to measure the ability of bacteria to metabolize different sources of carbon as a comparison tool for water samples.…”

Section: Microbial Metabolic Profilementioning

confidence: 99%

Farming and soil urban occupation in the water quality of Jaboticabal and Cerradinho streams

et al. 2016

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Since the end of the twentieth century, 100% of the urban sewage from the city of Jaboticabal has been collected by interceptors and routed to a treatment plant. Between 1999 and 2000, studies on the effect of this environmental care showed that it did not efficiently clean up the water from the two streams that run through the city and flow into an agricultural area. This paper focuses on assessing the influence of soil use on the water quality of surface waters from these two streams. The study was conducted 15 years after the implementation of sewage interceptors. The sampling dates were bimonthly at eight points (P1 to P8) in the Cerradinho and Jaboticabal streams, in Jaboticabal (São Paulo State, Brazil). P1 was located at the source of the Jaboticabal stream, P2 was in a farming area, P3, P4 and P5 were in an urban area, and P6, P7 and P8 were in a farming area. The physical and chemical variables of the water were assessed. We compared the ability of microorganisms to metabolize different sources of carbon using the EcoPlate (Biolog). The total phosphorus (TP) concentration exceeded the limit set by the Brazilian legislation as well as values found in previous studies, which was also observed for the chemical oxygen demand. However, the bacterial metabolic profile had no association with urban or farming practices. The results of the analysis indicated the possibility of clandestine discharge of wastewater in the streams studied and the influence of the agricultural soil.Index terms: Leaching; EcoPlate; metabolic profile; water resources. RESUMODesde o final do século vinte, 100% do esgoto urbano da cidade de Jaboticabal é recolhido por interceptores e conduzido a uma estação de tratamento. Entre 1999 e 2000, estudos sobre o efeito desse cuidado ambiental, mostraram que a coleta de esgoto ainda não tinha sido eficiente para despoluir as águas dos dois córregos que atravessam a cidade e fluem para uma área agrícola. Neste trabalho avalia-se a influência do uso do solo na qualidade da água destes dois córregos. O estudo foi realizado 15 anos após a implantação dos interceptores de esgoto. Durante o ano de 2014, bimestralmente, coletaram-se amostras de água em oito pontos (P1 a P8), dos córregos Cerradinho e Jaboticabal, em Jaboticabal (SP, Brasil). P1 estava localizado na nascente do córrego Jaboticabal; P2 em área agrícola; P3, P4 e P5 em área urbana; P6, P7 e P8 em área agrícola. Foram determinadas variáveis físicas e químicas e a comparação metabólica da população microbiana em diversas fontes de carbono pelo método EcoPlate (Biolog). Em todos os pontos e coletas, o teor de fósforo total excedeu o limite estabelecido pela legislação brasileira, assim como valores encontrados em estudos anteriores, o que também foi observado em relação à demanda química de oxigênio. Entretanto, a população microbiana da água parece não estar sendo alterada pelas atividades urbanas e agrícolas. Os resultados das análises sinalizam à possibilidade de descarga clandestina de águas residuárias nos córregos estudados e...

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The Application of the Biolog EcoPlate Approach in Ecotoxicological Evaluation of Dairy Sewage Sludge

Cited by 174 publications

References 33 publications

Zinc bioaccumulation by microbial consortium isolated from nickel smelter sludge disposal site

Zinc bioaccumulation by microbial consortium isolated from nickel smelter sludge disposal site

Effects of Different Land Use on Soil Bacterial Functional Diversity in Sanjiang Plain, Northeast China

Farming and soil urban occupation in the water quality of Jaboticabal and Cerradinho streams

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