Abstract:I n this work, new packings consisting of home made controlled porosity glass, new self-prepared aminoorganic silanes and vanillin as a ligand were used for affinity chromatography of some plant peroxidases (donor: H,O, oxidoreductases; EC 1.11.1.7). The procedure allowed simple and relatively high purification of enzymes. The resnlts were compared with those obtained with the material including commercially available y-aminopropyltriethoxysilane.
The separation and purification method of extracellular cellobiase and aryl-8-glucosidase fromTrichoderma reesei by means of affinity chromatography on controlled porosity glass activated by 7-aminopropyltriethoxysilane and oxiranes are described.
The separation and purification method of extracellular cellobiase and aryl-8-glucosidase fromTrichoderma reesei by means of affinity chromatography on controlled porosity glass activated by 7-aminopropyltriethoxysilane and oxiranes are described.
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