The application of micro-autoradiographic techniques to ecological studies

Brock, M. Louise; Brock, Thomas D.

doi:10.1080/05384680.1968.11903861

Cited by 31 publications

(26 citation statements)

References 19 publications

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“…Emulsion preparation, exposure, and development followed the liquid emulsion technique of Brock and Brock (1968). A 1:l dilution of Eastman Kodak NTB-2 liquid nuclear track emulsion and glass-distilled water was used (Paerl 1974).…”

Section: Methodsmentioning

confidence: 99%

Comparative photoheterotrophy, chemoheterotrophy, and photolithotrophy in a eutrophic reservoir and an oligotrophic lake1

Ellis

Stanford

1982

Limnology & Oceanography

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Autoradiography was used to determine the role in the uptake of NaH14C0, and [3111glucose of specific microorganisms of two sizes (0.45-10 pm and lo-165 Frn) in a eutrophic reservoir and an oligotrophic lake. Ultraplankton (0.45-10 pm) dominated plankton numbers and metabolic activity in both systems. An increase in photolithotrophy during the fall was due to an increase in the activity, numbers * ml .l, or both, of specific phytoplankters rather than an increase in the percentage of viable cells. Many of the most abundant species were not the most productive in tcrrns of inorganic C fixation. Single species dominated total inorganic C fixation in both systems.

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Section: Methodsmentioning

confidence: 99%

Comparative photoheterotrophy, chemoheterotrophy, and photolithotrophy in a eutrophic reservoir and an oligotrophic lake1

Ellis

Stanford

1982

Limnology & Oceanography

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“…One of the most powerful approaches to study physiological activities in mixed microbial assemblages dates back to the 1960s. The uptake of radiolabeled tracer substrates into individual cells can be visualized by a photographic technique termed microautoradiography (30,31) (Fig. 6a).…”

Section: Single-cell Activities Of Individual Populationsmentioning

confidence: 99%

Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations

Pernthaler

Amann

2005

Microbiol Mol Biol Rev

134

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Major biogeochemical processes in the water columns of lakes and oceans are related to the activities of heterotrophic microbes, e.g., the mineralization of organic carbon from photosynthesis and allochthonous influx or its transport to the higher trophic levels. During the last 15 years, cultivation-independent molecular techniques have substantially contributed to our understanding of the diversity of the microbial communities in different aquatic systems. In parallel, the complexity of aquatic habitats at a microscale has inspired research on the ecophysiological properties of uncultured microorganisms that thrive in a continuum of dissolved to particulate organic matter. One possibility to link these two aspects is to adopt a “Gleasonian” perspective, i.e., to study aquatic microbial assemblages in situ at the population level rather than looking at microbial community structure, diversity, or function as a whole. This review compiles current knowledge about the role and fate of different populations of heterotrophic picoplankton in marine and inland waters. Specifically, we focus on a growing suite of techniques that link the analysis of bacterial identity with growth, morphology, and various physiological activities at the level of single cells. An overview is given of the potential and limitations of methodological approaches, and factors that might control the population sizes of different microbes in pelagic habitats are discussed

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“…For example, direct observation of microbial C assimilation by individual microbial cells in environmental samples has been illustrated by using autoradiographic techniques (7,14). Although 13 N has been used as a powerful tool with which to elucidate reaction mechanisms (2), its relatively short half-life (9.96 min) (21) precludes its use for environmental studies, such as N assimilation by individual cells.…”

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confidence: 99%

Exploration of Inorganic C and N Assimilation by Soil Microbes with Time-of-Flight Secondary Ion Mass Spectrometry

Cliff

Gaspar

Bottomley

et al. 2002

Appl Environ Microbiol

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Stable C and N isotopes have long been used to examine properties of various C and N cycling processes in soils. Unfortunately, relatively large sample sizes are needed for accurate gas phase isotope ratio mass spectrometric analysis. This limitation has prevented researchers from addressing C and N cycling issues on microbially meaningful scales. Here we explored the use of time-of-flight secondary ion mass spectrometry (TOF-SIMS) to detect 13 ؊ . We observed that the 15 N content of fungal hyphae grown on the slides was significantly lower in regions where the hyphae were influenced by N-rich manure than in regions influenced by N-deficient straw. This effect occurred over distances of tens to hundreds of microns. Our data illustrate that TOF-SIMS has the potential to locate N-assimilating microorganisms in soil and to quantify the 15 N content of cells that have assimilated 15 N-labeled mineral N and shows promise as a tool with which to explore the factors controlling microsite heterogeneities in soil.

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The application of micro-autoradiographic techniques to ecological studies

Cited by 31 publications

References 19 publications

Comparative photoheterotrophy, chemoheterotrophy, and photolithotrophy in a eutrophic reservoir and an oligotrophic lake1

Comparative photoheterotrophy, chemoheterotrophy, and photolithotrophy in a eutrophic reservoir and an oligotrophic lake1

Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations

Exploration of Inorganic C and N Assimilation by Soil Microbes with Time-of-Flight Secondary Ion Mass Spectrometry

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