The Application of Lactobacillus reuteri CCM 8617 and Flaxseed Positively Improved the Health of Mice Challenged with Enterotoxigenic E. coli O149:F4

Andrejčáková, Zuzana; Vlčková, Radoslava; Hertelyová, Zdenka; Gancarčíková, Soňa; Nemcová, Radomí­ra

doi:10.1007/s12602-019-09578-x

Cited by 11 publications

(7 citation statements)

References 68 publications

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“…However, Lactobacillus are gram-positive bacteria that can promote the fermentation of carbohydrates into lactic acid. Moreover, the combination of Lactobacillus reuteri CCM8617 and flaxseed can increase the concentration of SCFAs in the intestines of mice, maintain the integrity of the mucosa, promote the growth of intestinal epithelial cells, have a biological antagonistic effect on the pathogenic bacteria E.coli O149:F4, and promote intestinal health ( 49 ). It is worth noting that Staphylococcus lentus , which was significantly enriched in the cecum after treatment with CB, is an animal pathogen ( 50 ).…”

Section: Discussionmentioning

confidence: 99%

Clostridium butyricum Alleviates Enterotoxigenic Escherichia coli K88-Induced Oxidative Damage Through Regulating the p62-Keap1-Nrf2 Signaling Pathway and Remodeling the Cecal Microbial Community

Shang

Liu

et al. 2021

Front. Immunol.

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Clostridium butyricum (CB) can enhance antioxidant capacity and alleviate oxidative damage, but the molecular mechanism by which this occurs remains unclear. This study used enterotoxigenic Escherichia coli (ETEC) K88 as a pathogenic model, and the p62-Keap1-Nrf2 signaling pathway and intestinal microbiota as the starting point to explore the mechanism through which CB alleviates oxidative damage. After pretreatment with CB for 15 d, mice were challenged with ETEC K88 for 24 h. The results suggest that CB pretreatment can dramatically reduce crypt depth (CD) and significantly increase villus height (VH) and VH/CD in the jejunum of ETEC K88-infected mice and relieve morphological lesions of the liver and jejunum. Additionally, compared with ETEC-infected group, pretreatment with 4.4×106 CFU/mL CB can significantly reduce malondialdehyde (MDA) level and dramatically increase superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels in the serum. This pretreatment can also greatly increase the mRNA expression levels of tight junction proteins and genes related to the p62-Keap1-Nrf2 signaling pathway in the liver and jejunum in ETEC K88-infected mice. Meanwhile, 16S rDNA amplicon sequencing revealed that Clostridium disporicum was significantly enriched after ETEC K88 challenge relative to the control group, while Lactobacillus was significantly enriched after 4.4×106 CFU/mL CB treatment. Furthermore, 4.4×106 CFU/mL CB pretreatment increased the short-chain fatty acid (SCFA) contents in the cecum of ETEC K88-infected mice. Moreover, we found that Lachnoclostridium, Roseburia, Lactobacillus, Terrisporobacter, Akkermansia, and Bacteroides are closely related to SCFA contents and oxidative indicators. Taken together, 4.4×106 CFU/mL CB pretreatment can alleviate ETEC K88-induced oxidative damage through activating the p62-Keap1-Nrf2 signaling pathway and remodeling the cecal microbiota community in mice.

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Section: Discussionmentioning

confidence: 99%

Clostridium butyricum Alleviates Enterotoxigenic Escherichia coli K88-Induced Oxidative Damage Through Regulating the p62-Keap1-Nrf2 Signaling Pathway and Remodeling the Cecal Microbial Community

Shang

Liu

et al. 2021

Front. Immunol.

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“…Animals of both groups were fed a standard diet for mice (Altromin 1311; VELAZ s.r.o.). The diet composition is the same as was published previously by Andrejčáková et al (2020). The animals had free access to water kept in glass bottles.…”

Section: Animals Housing and Dietsmentioning

confidence: 99%

Effects of supplemental flaxseed on the ovarian and uterine functions of adult cycling mice

Vlčková¹,

Andrejčáková²,

Kozioł³

et al. 2022

gpb

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“…We determined the activity of LDH in the extracts of liver homogenates. The LDH is an intracellular enzyme that after release to the surrounding environment indicates damage to tissues [4,5,22,40]. The activity of the total lactate dehydrogenase in the liver sample extracts was higher in groups X and XF in comparison to the control group C, although the difference was insignificant (Fig.…”

Section: Resultsmentioning

confidence: 97%

Protective Effect of Flaxseed on the Health of Experimental Animals Exposed to Xylene

2020

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AbstractXylene is mainly used as a solvent in the printing, tire and leather industries. It is also used as: a facility cleaner, paint and varnish thinner, component of fuel, and chemical for the laboratory processing of histological preparations. For these reasons people are frequently exposed to xylene and the risk of intoxication is high. This study focused on the protective effect of flaxseed on mice experimentally intoxicated with xylene. The experiment lasted 14 days. The mice used in this study (n = 60) were allocated to 3 groups: the control group (C) received only the standard diet; the xylene group (X) was fed a standard diet and was administered xylene p. o. (10 µl daily); and the xylene + flaxseed group (XF) received the standard feed, crushed flaxseed and xylene at the same dose as group X. The observations involved changes in: body weight, liver enzyme levels, and caspase activity in the liver of the mice. The administration of additives resulted in significant changes in the body weight of the mice on day 7 of the experiment (P < 0.05). The highest weight gain was observed in mice from the XF group. In contrast, the body weight of the mice from group X exposed only to xylene was the lowest. The biochemical analysis of the liver cells of the xylene intoxicated mice showed elevated levels of: aspartate aminotransferase (AST), De Ritis ratio (AST/ALT ratio), and lactate dehydrogenase isoenzymes LDH-3 and LDH-5. Caspase-3, the marker of apoptosis, was increased in the XF group. Thus, the administration of flaxseed in our experiment had a beneficial effect on the clinical and metabolic parameters of mice intoxicated with xylene. Our results indicated that the administration of flaxseed, may act as a preventative measure with respect to xylene intoxication of animals; however, further analyses are needed to confirm this assumption.

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The Application of Lactobacillus reuteri CCM 8617 and Flaxseed Positively Improved the Health of Mice Challenged with Enterotoxigenic E. coli O149:F4

Cited by 11 publications

References 68 publications

Clostridium butyricum Alleviates Enterotoxigenic Escherichia coli K88-Induced Oxidative Damage Through Regulating the p62-Keap1-Nrf2 Signaling Pathway and Remodeling the Cecal Microbial Community

Clostridium butyricum Alleviates Enterotoxigenic Escherichia coli K88-Induced Oxidative Damage Through Regulating the p62-Keap1-Nrf2 Signaling Pathway and Remodeling the Cecal Microbial Community

Effects of supplemental flaxseed on the ovarian and uterine functions of adult cycling mice

Protective Effect of Flaxseed on the Health of Experimental Animals Exposed to Xylene

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