The application of IL-10 and TNF-α in expressed prostatic secretions and prostatic exosomal protein in urine in the diagnosis of patients with chronic prostatitis

Yin, Lianli; Tang, Yinghua; Pan, Aihua; Yang, Lingfeng; Zhu, Xu; Liu, Yonggang

doi:10.1097/md.0000000000016848

Cited by 11 publications

(4 citation statements)

References 31 publications

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“…However, the expression levels of PSEP in patients with NIH‐II, NIH‐IIIa, and NIH‐IIIb were not statistically different ( p > 0.0125). It can be seen that PSEP is not useful for identifying the NIH classification subtypes of CP, which is consistent with our previous report 11,12 . This may be because CP is caused by a variety of etiologies, including complex pathological changes such as immunity, pathogenicity, and neuroendocrine inflammation.…”

Section: Discussionsupporting

confidence: 86%

The diagnostic value of urine heat shock protein 70 and prostatic exosomal protein in chronic prostatitis

Tang

Pan

Liu

et al. 2021

Clinical Laboratory Analysis

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Objective To explore the diagnostic value of the levels of prostatic exosomal protein (PSEP) and heat shock protein 70 (HSP70) in the urine of patients with chronic prostatitis (CP). Method Urine samples from 210 CP patients (70 cases of the USA National Institutes of Health Category II [NIH‐II], 70 NIH‐IIIa, and 70 NIH‐IIIb patients) and 70 control subjects were collected between May 2018 and February 2020. The levels of PSEP and HSP70 in urine were detected by enzyme‐linked immunosorbent assay. The differences in urine PSEP and HSP70 levels between the groups were analyzed, and receiver operating characteristic (ROC) curves were used to analyze the clinical value of PSEP and HSP70 in the diagnosis of CP. Results The PSEP levels of CP patients were significantly higher than those of the control group (p < 0.001), but there was no difference in PSEP levels among CP subgroups. The level of HSP70 in the urine of the NIH‐II patients was significantly lower than the levels in the NIH‐IIIa and NIH‐IIIb subgroups and the control group, but there was no difference in HSP70 levels between the NIH‐IIIa and NIH‐IIIb subgroups and the control group. ROC curve analysis results showed that the area under the curve (AUC) of PSEP for the NIH‐II, NIH‐IIIa, and NIH‐IIIb patients was 0.751, 0.776, and 0.731, respectively. The AUC of HSP70 in NIH‐II patients was 0.784, and the AUC of combined detection of PSEP and HSP70 in NIH‐II patients was 0.858. Conclusion Urine PSEP can be used as a marker for the diagnosis of CP, but it cannot distinguish between the various types of CP, and HSP70 can be used as a diagnostic index for NIH‐II classification.

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Section: Discussionsupporting

confidence: 86%

The diagnostic value of urine heat shock protein 70 and prostatic exosomal protein in chronic prostatitis

Tang

Pan

Liu

et al. 2021

Clinical Laboratory Analysis

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“…The ROC analysis links the predictivity of three inflammatory factors (IL-1 alpha, TNF-alpha and d-ROMs) with cellular elements that are present in reduced numbers in the seminal fluid and that increase in the case of infections [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Non-Andrological Medications on Semen Characteristics, Oxidative Stress and Inflammatory Parameters

et al. 2023

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Background and Objectives: The aim of this study was to evaluate the impact of medications on oxidative stress, inflammatory biomarkers and semen characteristics in males with idiopathic infertility. Materials and Methods: In this observational case-control clinical study, 50 men with idiopathic infertility were enrolled, of whom 38 (the study group) were on pharmacological treatment and 12 made up the control group. The study group was clustered according to the medications (Group A: anti-hypertensive, n = 10; Group B: thyroxine, n = 6; Group C: non-steroidal anti-inflammatory drugs, n = 13; Group D: miscellaneous, n = 6; Group E: lipid-lowering drugs, n = 4). Semen analyses were performed according to WHO 2010 guidelines. Interleukins (IL)-10, IL-1 beta, IL-4, IL-6, Tumor Necrosis Factor- alpha (TNF-alpha) and IL-1 alpha were determined using a solid-phase sandwich immunoassay. The diacron reactive oxygen metabolites, d-ROMs test, was performed by means of a colorimetric determination of reactive oxygen metabolites and measured with a spectrophotometer. Beta-2-microglobulin and cystatin-C were measured with an immunoturbidimetric analyzer. Results: No differences between the study and control groups for age and macroscopic and microscopic semen characteristics were found, nor were any differences found after clustering according to the drug categories. IL-1 alpha and IL-10 were significantly lower in the study group compared with the control group; IL-10 was significantly lower in groups A, B, C and D compared with the control group. Furthermore, a direct correlation between IL-1 alpha, IL-10 and TNF-alpha and leukocytes was found. Conclusions: Despite the sample size limitations, the data suggest a correlation between drug use and activation of the inflammatory response. This could clarify the pathogenic mechanism of action for several pharmacological classes on male infertility.

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“…Many of these targets have been shown to be closely associated with CP/CPPS, such as IL-6, AKT1, TNF. Overexpression of in ammatory cytokines and chemokines such as IL-1β, TNF-α, IL-2, IL-6, IL-8, MCP-1, et al have beenobserved in clinical samples from CP/CPPS patients [47][48][49][50][51][52]. These indicators released after in ammatory injury, which may increase the sensitivity of peripheral afferent nerves to stimulation and cause chronic pain [53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effects of Jiedu Huoxue Decoction in Chronic Prostatitis/Chronic Pelvic Pain Syndrome: Network Pharmacology, Molecular Docking and Experimental Evidence

Pan

Zhang

et al. 2022

Preprint

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Background: Jiedu Huoxue decoction (JDHXD) can be effectively alleviated chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) patients’ pelvic pain, lower urinary tract symptoms, and other discomforts. But its mechanism of action involving multi-component, multi-target and multi-pathway is unclear. Objectives: Integrated strategy of network pharmacological prediction, molecular docking, and experimental validation elucidate potential mechanism of JDHXD in treating CP/CPPS.Study design and methods: Two traditional Chinese medicine systems pharmacology databases and three disease related databases were applied to identify the herbs active components and the putative targets of CP/CPPS. Using the Cytoscape to constructed and analysis the herbs-compounds-targets network. Subsequently, the protein-protein interaction (PPI) was investigated by STRING database. Using DAVID databases to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. The binding of core active compounds with hub targets were assessed by molecular docking. Furthermore, in vivo efficacy of JDHXD was validated in CP/CPPS rat model. The key signal pathway of network pharmacological prediction would be validated by animal experiment.Result: In total, 149 active compounds of 10 Chinese herbals and 909 potential CP/CPPS-related targets were identified. Network pharmacology revealed 161 common targets between 136 potential active compounds and CP/CPPS. After topology analysis, 5 core active compounds and 18 hub targets have been screened. PPI analysis obtained 18 hub targets. Based on enrichment analysis of 161 targets, we obtained GO function 277 items and KEGG pathway 114 items. Molecular docking verification shown that the top 5 hub targets were well binding with 5 core active compounds. Animal experiment shown that JDHXD had protective effect on CP/CPPS. JDHXD can significantly down-regulated the key gene expression (JNK, ERK1/2 and P38) and inhibit phosphorylation of ERK1/2，JNK and P38 in the MAPK signaling pathway.Conclusions: This study demonstrated potential mechanism of JDHXD in treating CP/CPPS. Network pharmacological, molecular docking and experimental verification revealed its multi-component, multi-target and multi-pathway treatment characteristics. The reliability and effectiveness of those method are very helpful to explore the material basis and mechanism of traditional Chinese medicine.

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The application of IL-10 and TNF-α in expressed prostatic secretions and prostatic exosomal protein in urine in the diagnosis of patients with chronic prostatitis

Cited by 11 publications

References 31 publications

The diagnostic value of urine heat shock protein 70 and prostatic exosomal protein in chronic prostatitis

The diagnostic value of urine heat shock protein 70 and prostatic exosomal protein in chronic prostatitis

The Impact of Non-Andrological Medications on Semen Characteristics, Oxidative Stress and Inflammatory Parameters

Therapeutic Effects of Jiedu Huoxue Decoction in Chronic Prostatitis/Chronic Pelvic Pain Syndrome: Network Pharmacology, Molecular Docking and Experimental Evidence

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