The application of HPLC ESI MS in the investigation of the flavonoids and flavonoid glycosides of a Caribbean Lamiaceae plant with potential for bioaccumulation

Abstract

As part of an exchange technology program between the government of Barbados and Environment Canada, methanolic and aqueous extracts from the flavonoid-rich Lamiaceae family were characterized using negative-ion electrospray mass spectrometry. The species investigated is part of the Caribbean Pharmacopoeia, and is used for a variety of health issues, including colds, flu, diabetes, and hypertension. The extracts were investigated for structural elucidation of phenolics, identification of chemical taxonomic profile, and evidence of bio-accumulator potential. The methanolic and aqueous leaf extracts of Plectranthus amboinicus yielded rosmarinic acid, ladanein, cirsimaritin, and other methoxylated flavonoids. This genus also shows a tendency to form conjugates with monosaccharides, including glucose, galactose, and rhamnose. The aqueous extract yielded four isomeric rhamnosides. The formation of conjugates by Plectranthus amboinicus is thus evidence of high bioaccumulator significance.

Insights into the neuroprotective effects ofSalvia officinalisL. andSalvia microphyllaKunth in the memory impairment rat model

“…Tentative identification was achieved by comparison of retention times (R t ) and mass spectra with reported data. [20][21][22][23]…”

Characterization and identification of the major flavonoids in Phyllostachys edulis leaf extract by UPLC–QTOF–MS/MS

“…Mass spectrometry (MS) has been widely used for the structural determination of flavonoids [21,22]. Liquid chromatography combined with tandem mass spectrometry (LC-MS) has been shown to be a valuable technique with high sensitivity and high resolution; recently, it has been widely applied to the structural characterization and isomeric differentiation of flavonoids using both positive and negative ion modes [23][24][25][26]. The application of mass spectrometry in the structural analysis of flavonoids has been reviewed in detail [27][28][29].…”

HPLC-Esi-MS Analysis of Some Bioactive Substances in Two Yemeni Medicinal Plants

“…Previously, researchers dedicated their efforts to study phytochemistry, traditional uses, side effects, and future perspectives of P. amboinicus; investigate of the influence of different solvents to recover higher phytochemicals from a local P. amboinicus and GC-MS analysis of bioactive nonvolatile compounds; identify of essential oil compositions of P. asirensis analyzed by various gas chromatography techniques (GC-MS, GC-FID) using two different stationary phase columns (polar and nonpolar) and HPLC-PDA profiling of phenolic constituents; and isolate, identify and quantity of the major compounds using high resolution UPLC-MS analysis, [44][45][46][47][48][49]. The recent work however was performed using HPLC-MS operated in positive ion mode for two analyzed plants (Figs.…”

“…As the coupling of HPLC with MS is possible through ESI ionization source [46,50], analysis of a methanolic extract of P. asirensis and P. amboinicus plants by this technique detected numerous bioactive compounds some of them are arranged in Tables 2 and 3.…”

“…Whereas the development of electrospray ionization-mass spectrometry (ESI-MS) became very valuable in the formation of gas-phase ions from large biologically important macromolecules and analysis, structural characterization as well as identification based on the basis of molecular mass. [43] As far as we know, some medicinal plants such as Plectranthus asirensis and Plectranthus amboinicus have rarely mentioned in literatures refer to their chemical components or the analysis processes [2,[44][45][46][47][48][49] and we believe in this aspect it is the time to study their natural components.…”