The application of fully automated on-line solid phase extraction in bioanalysis

Mitchell, Richard J.; Christian, Rachelle; Hughes, Heather; Miah, Asadh; Walker, Don K.

doi:10.1016/j.jpba.2009.11.002

Cited by 17 publications

(9 citation statements)

References 8 publications

(6 reference statements)

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“…For example, this approach was used in trapping and separating peptides in limited amounts of aqueous microdialysate from muscle tissue, rat brain extracts and serum tryptic digests (Rogeberg et al, ). Comparison between on‐line and off‐line SPE‐HPLC systems showed that they are comparable in analytical performance, although on‐line methodologies are characterized by economic advantages for the analysis of large numbers of samples with a marked reduction in manual operations (Mitchell et al, ).…”

Section: Solid‐phase Extraction With Nonselective Adsorbentsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

“…Besides its ability to concentrate and isolate analytes from complex samples in automated off-line fashion, SPE can be used in on-line configuration with the HPLC system (Mitchell, Christian, Hughes, Miah, & Walker, 2010). Both on-line and off-line methods have been evaluated for linearity, carryover, precision and accuracy, and mainly the matrix effects for ESI-MS detection used for bioanalysis purposes (Mitchell et al, 2010). Owing to its robustness, SPE is recognized as an alternative technique to LLE-based techniques, being superior in terms of speed, extraction efficiency, sample size required, automation and the possibility of being coupled on-line with the chromatographic system (Marczylo, Lam, Nallendran, Taylor, & Konje, 2009).…”

Section: )mentioning

confidence: 99%

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Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Medvedovici

Bacalum

David

2017

Biomedical Chromatography

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Quality of the analytical data obtained for large-scale and long term bioanalytical studies based on liquid chromatography depends on a number of experimental factors including the choice of sample preparation method. This review discusses this tedious part of bioanalytical studies, applied to large-scale samples and using liquid chromatography coupled with different detector types as core analytical technique. The main sample preparation methods included in this paper are protein precipitation, liquid-liquid extraction, solid-phase extraction, derivatization and their versions. They are discussed by analytical performances, fields of applications, advantages and disadvantages. The cited literature covers mainly the analytical achievements during the last decade, although several previous papers became more valuable in time and they are included in this review.

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Section: Solid‐phase Extraction With Nonselective Adsorbentsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Section: )mentioning

confidence: 99%

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Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Medvedovici

Bacalum

David

2017

Biomedical Chromatography

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“…6 However, one of the challenges for the application of chromatography technology in TDM is the sample pretreatment. [10][11][12][13][14][15][16][17][18][19] A number of methods for the determination of these three analytes in biological uids by LC-MS/MS individually or in combination have been described 20-28 using conventional method such as protein precipitation, offline solid phase extraction and liquid-liquid extraction for sample pretreatment that were time-consuming, labor intensive and costly. To remove biological matrix, sample pretreatment is essential.…”

Section: Introductionmentioning

confidence: 99%

A simple and automated online SPE-LC-MS/MS method for simultaneous determination of olanzapine, fluoxetine and norfluoxetine in human plasma and its application in therapeutic drug monitoring

Fan

Shen

et al. 2015

RSC Adv.

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The olanzapine-fluoxetine augmentation strategy has been proved efficacious for treatment-resistant depression, psychotic depression and bipolar depression. To achieve efficient therapeutic drug monitoring (TDM), we develop an automated online SPE-LC-MS/MS method for the simultaneous quantification of olanzapine, fluoxetine and norfluoxetine in human plasma. After adding an internal standard of diphenhydramine and centrifugation, a 10 mL plasma sample was directly injected into the SPE cartridge. While the analytes were retained on the SPE cartridge, the endogenous materials were washed out by the loading solvent. Following the valve switching, the analytes were eluted from the SPE cartridge to the analytical column by gradient elution. The analytes were quantified using a triplequadrupole tandem mass spectrometer. Calibration curves were linear over the concentration range of 0.25-50.00 ng mL À1 for olanzapine, and 0.50-100.00 ng mL À1 for fluoxetine and norfluoxetine. The intra-and inter-day precisions were within 1.17% and 4.63%. And the accuracies were between 95.60% and 101.48%. The mean matrix effect was in the range of 90.35% to 99.89% and mean recovery was in the range of 90.35% to 96.99%. This method has been successfully applied to two Chinese schizophrenia patients. The online SPE-LC-MS/MS method allows sensitive and robust quantification of olanzapine, fluoxetine and norfluoxetine for routine TDM in clinic.

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“…Increased demands for faster data turnaround times (e.g., within 24 h of sample receipt) have highlighted that the sample preparation stage has become the rate-limiting step in the analysis process. This has driven the need to explore alternative approaches, such as the automation of liquid-liquid extraction, supported liquid extraction (SLE), protein precipitation and online extraction systems (e.g., micro-turbulent flow chromatography [µTFC] [1][2][3][4][5][6] and symbiosis [7][8]). Minimizing method development and sample preparation times through the use of generic µTFC systems and protein precipitation has proven advantageous in reducing sample extraction cycle times in quantitative bioanalysis.…”

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confidence: 99%

Automated Micropipette Tip-Based SPE in Quantitative Bioanalysis

Luckwell¹,

Beal

2011

Bioanalysis

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The application of fully automated on-line solid phase extraction in bioanalysis

Cited by 17 publications

References 8 publications

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

A simple and automated online SPE-LC-MS/MS method for simultaneous determination of olanzapine, fluoxetine and norfluoxetine in human plasma and its application in therapeutic drug monitoring

Automated Micropipette Tip-Based SPE in Quantitative Bioanalysis

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