The application of Fourier-transform infrared (FTIR) and Raman spectroscopy (FTR) to the evaluation of structural changes in wool fibre keratin after deuterium exchange and modification by the orthosilicic acid

Wojciechowska, Elżbieta; Włochowicz, A.; Wysocki, Marian; Pielesz, Anna; Wesełucha–Birczyńska, Aleksandra

doi:10.1016/s0022-2860(02)00276-4

Cited by 24 publications

(10 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 4 showed the chicken feathers incubated by adding culture strain B6 resulted in the peaks of disulphide bonds become weak. Disulphide bonds owing to the S-S stretching vibrations show a peak in the 500-550cm -1 (Wojciechowska et al, 2004;Wojciechowska et al, 2002). The peaks appear in the range of 523-553 cm -1 as shown in Figure 6, which represents disulphide bonds present in the sample.…”

Section: Ftir Analysis Of Degradation Productmentioning

confidence: 92%

PROMOTING KERATINASE ACTIVITY FROM NEWLY IDENTIFIED STRAIN Strenotrophomonas maltophilia B6 THROUGH OPTIMIZATION AND CHARACTERIZATION

Mamangkey

Suryanto

Munir

et al. 2020

MABJ

View full text Add to dashboard Cite

Strenotrophomonas maltophilia B6 was a newly identified keratinolytic bacteria isolated from a landfill in North Sumatra. Keratinolytic activitiy of this strain was investigated through optimization and characterization of its enzymatic properties. Amino acid profiles were quantified using High Performance Liquid Chromatography (HPLC) after a 96-h of incubation resulted in 2.2569 mg mL-1 amino acids from a 40-mL fermentation supplemented with 10 g/L chicken feathers. The keratinolytic activities were enhanced through addition of 1% glucose yielded 67.41 ± 1.1 UmL-1 and 0.5% peptone yielded 90.24 ± 0.7 UmL-1. Keratinolytic activity enhanced with the addition of Ca+ (116%), Na+ (111%), Mg+ (110%) at 10 mM concentration. Zymogram analysis showed a protein band in the 26 kDa of molecular mass, similar to serine protease. Physical and chemical characteristics of degradation products were analyzed using Scanning Electron Microscope (SEM) and Fourier Transform Infra Red (FTIR). Qualitatively, the optimum activity occurred at 96-h of incubation through SEM image analysis. The FTIR peaks detected were peak 3747 cm-1 (-OH); peak 2972 cm-1 (asymmetric -CH3); peak 1641 cm-1 (amide I); peak 1438 cm-1 (amide II); peak 1092 cm-1 (-C-C-); peak 523 cm-1 (-S-S-). Thus, Strenotrophomonas maltophilia B6 may be used as potential biodegradative bacterium to chicken feather wastes in nature.

show abstract

Section: Ftir Analysis Of Degradation Productmentioning

confidence: 92%

PROMOTING KERATINASE ACTIVITY FROM NEWLY IDENTIFIED STRAIN Strenotrophomonas maltophilia B6 THROUGH OPTIMIZATION AND CHARACTERIZATION

Mamangkey

Suryanto

Munir

et al. 2020

MABJ

View full text Add to dashboard Cite

show abstract

“…Rather such groups are often identified through Raman spectroscopy [15][16][17][18], where such bands are typically stronger than in FTIR and NIR. Though useful in following chemical changes that occur in wool proteins [19][20][21][22], FTIR-ATR is ultimately destructive, requiring sacrificial samples that are not always accessible in historic collections. For historic tapestries, it is not possible to take samples from the front of the tapestry.…”

Section: Chemical Stability and The Oxidation Of Historic Wool Fibresmentioning

confidence: 99%

“…The oxidation products formed as a result of cystine scission can result in further fibre degradation through acidic hydrolysis and autocatalytic breakdown processes [6,41]. Through the examination of the cystine breakdown products and peak intensity changes, the extent of protein degradation can be inferred [20][21][22]. The NIR measurements had already been recorded for the historic tapestry weft yarns in McCullough's work and are relevant for this research as they were used to calibrate the PLS models against the oxidation products measured in this study.…”

Section: Research Aimsmentioning

confidence: 99%

Developing a non-invasive tool to assess the impact of oxidation on the structural integrity of historic wool in Tudor tapestries

et al. 2017

View full text Add to dashboard Cite

Near infrared spectroscopy (NIR) can be a useful diagnostic tool in the assessment of quality in textile, agricultural and various other industries. In cultural heritage applications, NIR as a non-invasive assessment tool is advantageous for understanding the composition or degradation of fragile historic objects that cannot be sampled. The direct interpretation of measured NIR spectra is complicated by overlapping bands produced from overtone and combination bands of chemical bonds. However, in combination with multivariate partial least squares (PLS) models and principal component analysis, the physicochemical properties of historic materials can potentially be identified by calibrating against a measured component. In this research, wool fibres from a sacrificial collection of historic tapestry fragments housed at Hampton Court Palace were analysed. The cystine oxidation products of historic wools which can be related to the embrittlement of fibres and ultimately fibre loss, were recorded using Fourier transform infrared (FTIR) spectroscopy. A PLS predictive model was then developed using previously measured NIR spectra for the same historic tapestry fragments to predict the ratios of the cystine oxidation species in historic wools measured in this research. The prediction of a validation set of historic tapestry fragments with known oxidation product ratios was found to be accurate to root mean squared errors of prediction (RMSEP) of 0.11 for cysteic acid/cystine dioxide, 0.03 for an oxidation ratio of cysteic acid/cystine monoxide and 0.06 for cystine dioxide/cystine monoxide. The model was applied successfully to an independent set of historic tapestry fragments with unknown oxidation ratios with an M-distance pass limit of 3. The models were also applied to NIR measurements of historic tapestries on open display with limited success. Alternative methods to investigate and account for errors in this research are proposed. The potential for NIR to be used by conservators as a non-invasive tool for the assessment of textiles, complementing current condition audit practices in use at heritage institutions is also discussed.

show abstract

“…11 The characteristic peak of amide II, induced by the bending vibration of N-H and stretching vibration of C-N, is at 1540 cm À1 ; at 1230 cm À1 , there is the characteristic absorption peak of amide III, which is induced by the C-C stretching vibration and C]O bending vibration. 4,[11][12][13][14][15]23 According to the literature, the wavelength from 1000 to 1170 cm À1 corresponds to the obvious changing of the aging wool ber. The characteristic peak at 1021 cm À1 indicates the Bunte salt group, and the characteristic peak at 1041 cm À1 represents cysteic acid (-SO 3 -).…”

Section: Ftir Spectroscopy Analysismentioning

confidence: 99%

Application of electron paramagnetic resonance spectroscopy, Fourier transform infrared spectroscopy-attenuated total reflectance and scanning electron microscopy to the study of the photo-oxidation of wool fiber

Zeng

et al. 2015

Anal. Methods

View full text Add to dashboard Cite

show abstract

The application of Fourier-transform infrared (FTIR) and Raman spectroscopy (FTR) to the evaluation of structural changes in wool fibre keratin after deuterium exchange and modification by the orthosilicic acid

Cited by 24 publications

References 7 publications

PROMOTING KERATINASE ACTIVITY FROM NEWLY IDENTIFIED STRAIN Strenotrophomonas maltophilia B6 THROUGH OPTIMIZATION AND CHARACTERIZATION

PROMOTING KERATINASE ACTIVITY FROM NEWLY IDENTIFIED STRAIN Strenotrophomonas maltophilia B6 THROUGH OPTIMIZATION AND CHARACTERIZATION

Developing a non-invasive tool to assess the impact of oxidation on the structural integrity of historic wool in Tudor tapestries

Application of electron paramagnetic resonance spectroscopy, Fourier transform infrared spectroscopy-attenuated total reflectance and scanning electron microscopy to the study of the photo-oxidation of wool fiber

Contact Info

Product

Resources

About