Treatment of otitis media with effusion (OME) in most cases requires topical application of medications, which are designed to stop the inflammatory process and act on exudate in the middle ear cavity. One of the most common treatments for OME is catheterization of the auditory tube using a Guillot catheter, which is not always effective. This work presents the second stage of the study on the introduction of drugs into the middle ear using an original ear catheter. The study included patients with OME (N = 14, the duration of the disease from 2 to 3 months). The patients underwent surgical treatment: bypass of the eardrum, septoplasty, turbinoplasty under endotracheal anesthesia. An intraoperative experiment was conducted to evaluate the effectiveness of catheterization of the auditory tube with an original catheter, a tinted solution and Miramistin solution. In all 14 cases, the eardrum of the affected ear during catheterization made oscillatory movements, synchronously with the reciprocating movements of the syringe plunger. In 2 cases (14.3%), we did not visualize the tinted solution behind the eardrum. The full filling of the tympanic cavity with tinted saline was 4 patients (28.6%), while the average volume of the injected solution (V) was 4.09 ml, and in 8 ears (57.1%) the tympanic cavity was partially filled with the contrast solution (V = 7.0 ml). In case of transtubar injection of Miramistin solution in the tympanic cavity in all cases (14 ears), the antiseptic solution enters the external auditory canal after myringotomy. By correlating the average volume of the tympanic cavity with the volume of the injected drug necessary for the initial filling of the tympanic cavity, the minimum loss of the liquid form of the drug was 1: 2.