The application of cholinesterase potentiometric biosensor for preliminary screening of the toxicity of waste waters

Evtugyn, Gennady; Rizaeva, E. P.; Stoikova, E. E.; Latipova, V. Z.; Budnikov, H. C.

doi:10.1002/elan.1140091417

Cited by 26 publications

(9 citation statements)

References 22 publications

(8 reference statements)

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“…Coumaphos and chloropyrifos-methyl in aqueous solutions and spiked grape juice were electrochemically oxidized to their oxygen analogues in accordance with the procedure reported on literature [32]. The electrolysis was performed in the presence of 0.1 mol L -1 NaCl on Pt electrodes (on-load voltage 6 V).…”

Section: Inhibition Measurementsmentioning

confidence: 99%

Cholinesterase sensors based on screen-printed electrodes for detection of organophosphorus and carbamic pesticides

Иванов

Evtugyn

Budnikov

et al. 2003

Analytical and Bioanalytical Chemistry

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Cholinesterase sensors based on screen-printed electrodes modified with polyaniline, 7,7',8,8'-tetracyanoquinodimethane (TCNQ), and Prussian blue have been developed and tested for detection of anticholinesterase pesticides in aqueous solution and in spiked grape juice. The influence of enzyme source and detection mode on biosensor performance was explored. It was shown that modification of the electrodes results in significant improvement of their analytical characteristics for pesticide determination. Thus, the slopes of the calibration curves obtained with modified electrodes were increased twofold and the detection limits of the pesticides were reduced by factors of 1.6 to 1.8 in comparison with the use of unmodified transducers. The biosensors developed make it possible to detect down to 2 x 10(-8) mol L(-1) chloropyrifos-methyl, 5 x 10(-8) mol L(-1) coumaphos, and 8 x 10(-9) mol L(-1) carbofuran in aqueous solution and grape juice. The optimal conditions for grape juice pretreatment were determined to diminish interference from the sample matrix.

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Section: Inhibition Measurementsmentioning

confidence: 99%

Cholinesterase sensors based on screen-printed electrodes for detection of organophosphorus and carbamic pesticides

Иванов

Evtugyn

Budnikov

et al. 2003

Analytical and Bioanalytical Chemistry

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“…Among electrochemical methods, the pH-shift potentiometry has been used by several investigators (see e.g., [8,9] and references herein). The disadvantage of this approach consists in necessity to use low buffer capacity of the sample.…”

Section: Introductionmentioning

confidence: 99%

Acetylcholine Biosensor Based on Dendrimer Layers for Pesticides Detection

et al. 2003

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We tested a new design of an enzyme biosensor based on acetylcholinesterase (AChE) and choline oxidase (ChO) immobilized on the supported monomolecular layer composed of poly(amidoamine) (PAMAM) dendrimers of the fourth generation (G4) mixed with 1-hexadecanethiol (HDT). The resulting enzymatic activity, measured amperometrically, was substantially depressed in the presence of the organophosphate pesticide dimethyl-2,2-dichlorovinylphosphate (DDVP, Dichlorvos), carbamate pesticides carbofuran and carbamate drug eserine. The detection limits (1.3 Â 10 À 3 ppb for DDVP, 0.01 ppb for carbofuran and 0.03 for eserine) were considerably lower than so far reported for AChE based amperometric and potentiometric sensors. The relative simple protocol of biosensor preparation, high sensitivity and stability is very promising for determination of environmental pollutants in field conditions.

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“…In this approach, the inhibition degree is compared with the results obtained with conventional toxicity assay techniques, i.e., acute toxicity on Daphnia magna or the Ames test of genotoxicity (Evtugyn et al 1997). This makes it possible to avoid the necessity of the information on the chemical content of the sample.…”

Section: From Chemical Weapons Toward Pesticidesmentioning

confidence: 99%

How Does It Work? Case Studies

Evtugyn

2013

Lecture Notes in Chemistry

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In this chapter, we deal mainly with the aspects of biosensor development related to specific areas of their application. It is definitely clear that biosensors that perform well in the laboratory can be utterly inappropriate outside the university walls. The gap between the laboratory pattern and a commercial product is intuitively understandable. The end-users want to receive a device that is robust, costeffective, simple to operate, and as sensitive as required for its purpose. As regards biosensors, the following aspects are imperative for the various stages of commercialization:• Stability of the biological component. The satisfactory working period estimated from the stability of the calibration parameters begins after six months. This is a rather mild estimate accepted by the medical services that are used to maintaining the unstable biochemicals that are necessary for conventional bioassays. In other areas, e.g., industry or environmental monitoring, the requirements can be stricter.• Operation/functioning convenience. To some extent, this is implied from the previous statements. A limited period of operation simply means the replacement of the sensing elements performed by an unqualified staff or an inexperienced buyer. In addition, mass production is hardly compatible with a complicated, multi-stage protocol of measurement. The simpler (faster, cheaper), the better. The producer should take into account that the customer might not be familiar with the specific conditions of biochemical reactions and will make very simple mistakes that a biochemist can't possibly make (buffers, order of reagents addition, temperature regime, etc.).• Analytical characteristics and metrology. The more complicated the sensor, the more difficult to establish the expected accuracy of the measurement. Concerning real samples assay, the main problems are focused on selectivity and signal drift. The biochemical recognition is sensitive enough to compete with most of the conventional instrumentation techniques, and this is one of the undisputable benefits always mentioned in research related to biosensor development. However, in real sample testing, the selectivity of the transducer can seriously limit the prospects of biochemical recognition. Careful consideration G. Evtugyn, Biosensors: Essentials, Lecture Notes in Chemistry 84,

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The application of cholinesterase potentiometric biosensor for preliminary screening of the toxicity of waste waters

Cited by 26 publications

References 22 publications

Cholinesterase sensors based on screen-printed electrodes for detection of organophosphorus and carbamic pesticides

Cholinesterase sensors based on screen-printed electrodes for detection of organophosphorus and carbamic pesticides

Acetylcholine Biosensor Based on Dendrimer Layers for Pesticides Detection

How Does It Work? Case Studies

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