The application of CD73 in minimal residual disease monitoring using flow cytometry in B-cell acute lymphoblastic leukemia

Abstract

The expression of CD73 by flow cytometry (FC) in bone marrow (BM) specimens of B-cell acute lymphoblastic leukemia (B-ALL) with or without minimal residual disease (MRD) was studied, and its advantages were evaluated using the MRD assay. This study also detected the expression profile of CD73 in hematogones and mature B cells in BM specimens of 18 healthy donors. Results showed that the mean value of CD73 expression in MRD-positive B cells was 6-fold greater than that in the MRD negative ones. Also, 41.82% MRD-positive B-ALL cases expressed high CD73 and the sensitivity of CD73-based MRD detection reached 10(-4). Since the expression of CD73 increases with the maturation of normal B cells, it is better to mix it with CD34, CD10 and CD20 in one tube to prevent the disturbance of mature B cells. CD73 is recommended as an optional MRD marker for B-ALL patients by using FC.

Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia

“…[32][33][34][35][36] Based on initial testing on diagnostic BCP-ALL samples, CD73 and CD304 appeared to be most promising based on the level and frequency of overexpression (;20% for CD73 and ;40% for CD304) and their stability during follow-up (data not shown). Because it appeared not to be possible to combine these 2 markers with CD66c and CD123 in a single fluorescence channel (due to too high background levels), a second tube was designed; this tube was identical to tube 1 but with CD73/CD304 instead of CD66c/CD123 in the PE channel ( Table 2).…”

“…38 These cases are rare and frequently present with a pro-B-ALL immunophenotype that can relatively easily be distinguished from normal BCP cells and plasma cells. 39 In our study, we tested and finally selected 4 markers that are frequently abnormally expressed on BCP-ALL cells: CD66c (associated with BCR-ABL and hyperdiploidy), 40,41 CD123 (associated with hyperdiploidy 42 ), CD73, 36 and CD304 (possibly associated with TEL-AML1). 33 By combining 2 of these markers in a single fluorescence channel, abnormal expression could be identified in ;70% of BCP-ALL patients.…”

Role of CD81 and CD58 in minimal residual disease detection in pediatric B lymphoblastic leukemia

“…Adoption of flow cytometry MRD is becoming routine in many flow cytometry laboratories. Numerous markers have been proposed in the literature as being informative in detecting MRD in B‐ALL, yet few studies have compared the performance of these markers in routine clinical practice.…”

“…All three populations, pre-B1 (blue), pre-B2 (red), and mature, were plotted CD38 versus CD81 in tube 1 (second row) and CD58 versus CD81 in tube 2 (third row) [Colour figure can be viewed at wileyonlinelibrary.com] cytometry MRD is becoming routine in many flow cytometry laboratories. Numerous markers have been proposed in the literature as being informative in detecting MRD in B-ALL, [11][12][13][14] yet few studies have compared the performance of these markers in routine clinical practice.…”

“…11 Few studies have evaluated the significance of these markers in detecting MRD by flow cytometry. CD58 has been proposed as playing a role in flow cytometry MRD 12,13 and is currently used in the COG protocol. 2 Similarly, decreased expression of CD81 was shown as a sensitive and specific marker.…”