The Application of Biotechnology to Orchids

Hossain, M. M.; Kant, Ravi; Van, Pham Thanh; Winarto, Budi; Zeng, Songjun; Silva, Jaime A. Teixeira da

doi:10.1080/07352689.2012.715984

Cited by 126 publications

(75 citation statements)

References 417 publications

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“…PLBs, having higher capabilities to regenerate into plantlets, are the preferred target tissues in cryopreservation due to the large number of PLBs that can be obtained within a relatively short period of time (Sreeramanan et al, 2008;Anthony et al, 2011). PLBs serve as models for somatic embryos in orchids (Hossain et al, 2013;Teixeira, 2013a). When PLBs are encapsulated, the resulting synthetic seeds (Sharma et al, 2013;Teixeira, 2013b) can be used for cryopreservation (Teixeira, 2012).…”

Section: Discussionmentioning

confidence: 99%

An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies

Rajasegar¹,

Mansor²,

Poobathy³

et al. 2015

Turk J Biol

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Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate (53.3%) was obtained when the PLBs were subjected to a 7-day dark treatment before being transferred to a 16-h/8-h light/dark photoperiod. Histological analyses were conducted to study the morphology of cryopreserved and noncryopreserved PLBs of Ascocenda Wangsa Gold. The vitrification protocol developed in this study is a feasible and safe method for strengthening the germplasm conservation of this orchid for commercial purposes.

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Section: Discussionmentioning

confidence: 99%

An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies

Rajasegar¹,

Mansor²,

Poobathy³

et al. 2015

Turk J Biol

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“…They observed that the total phenol content increased with increasing BA concentration. Hossain et al (2013) . studied in vitro propagation of Malaxis khasiana through immature seed culture They rapidly propagated this plant by taking seeds of 8-9 weeks after pollination9WAP) cultured on MS medium by using 2% sucrose supplemented with 500 mg/l casein hydrolysate 1µM BA exhibited germination of 75% seeds after 107 days.…”

Section: Figure1 Creipidium Acuminatum (Ddon) Szlach In Natural Habmentioning

confidence: 99%

A Review On In Vitro Propagation and Phytochemical Analysis of Ethnobotanically Important Orchid Crepidium Acuminatum (D.Don) Szlach.

Arora¹,

Singh²,

Mahajan³

et al. 2017

IOSRJPBS

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“…Many authors states that PLBs are somatic embryos (Hossain et al, 2013;Lee et al, 2013), or that somatic embryogenesis is the initial step on the PLBs formation (Zhao, Wu, Feng, & Wang 2008). Through histological and histochemical studies, Lee et al (2013) demonstrated that during the early stages of PLB formation, the cells show cytological characteristics and cell wall markers similar to zygotic embryo development.…”

Section: Introductionmentioning

confidence: 99%

“…Considered a biodiversity hotspot, it holds about 20,000 different plant species, of which 8,000 are endemic (Myers, Mittermeier, Mittermeier, Da Fonseca, & Kent, 2000). The family Orchidaceae includes 27,801 species distributed in 899 genera (The Plant List, 2013), being one of the largest and specialized families in the plant kingdom, spread throughout almost all continents, especially in tropical and subtropical regions (Hossain et al, 2013). Cattleya tigrina A. Richard is an endemic orchid species to Atlantic forest biome, and classified as vulnerable in the Red Book of Brazilian Flora (Martinelli & Moraes, 2013 In vitro micropropagation techniques have been a widely used tool for conservation of endangered plant species (Sarasan et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

Almeida

Fraga

Navarro

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in culture, coinciding with a subtle change in the structures morphogenetic development. During PLBs induction, putrescine exhibited higher levels as compared to spermidine and spermine, and apparently presents a major role during the PLBs induction in C. tigrina. Due to the apparent secondary PLBs formation, this protocol can represent a highly efficient method for in vitro propagation of this species.Keywords: orchids, micropropagation, in vitro culture, epigenetics, PLBs.Dinâmica da metilação do DNA global e níveis endógenos de poliaminas durante a indução de estruturas semelhantes a protocormos de Cattleya tigrina A. Richard RESUMO. Cattleya tigrina é uma espécie endêmica do bioma Mata Atlântica e classificada como vulnerável no Livro Vermelho da Flora Brasileira. As técnicas in vitro compreendem ferramentas valiosas a serem empregadas na conservação de espécies de plantas ameaçadas. O objetivo do presente estudo foi avaliar as características morfológicas, os níveis globais de metilação do DNA e as poliaminas livres durante a indução de estruturas semelhantes a protocormos (ESPs). Paralelamente, um protocolo eficiente para a propagação in vitro desta espécie é apresentado. As primeiras evidências de indução de ESPs em C. tigrina foram observadas aos sete dias de cultivo, a partir da porção basal da superfície abaxial da folha. Uma hipometilação foi observada concomitante ao início da diferenciação celular, e um aumento da metilação global do DNA foi encontrada aos 35 dias de cultivo, coincidindo com uma sutil mudança no desenvolvimento morfogenético das estruturas. Durante a indução de ESPs, a putrescina exibiu níveis aumentados em comparação a espermidina e espermina e, aparentemente, apresenta um papel importante durante a indução dessas estruturas em C. tigrina. Devido à aparente formação secundária de ESPs, este protocolo pode representar um método altamente eficiente para a propagação in vitro desta espécie.Palavras-chave: orquideas, micropropagação, cultivo in vitro, epigenética, ESPs.

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The Application of Biotechnology to Orchids

Cited by 126 publications

References 417 publications

An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies

An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies

A Review On In Vitro Propagation and Phytochemical Analysis of Ethnobotanically Important Orchid Crepidium Acuminatum (D.Don) Szlach.

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

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