The application of asymmetric PCR-SSCP in gene mutation detecting

Abstract

The advantages and disadvantages of asymmetric PCR-SSCP and the traditional PCR-SSCP were compared in this study. The mutations in 39UTR of Smad4 gene of Luxi cattle and the Holstein cow were analyzed by asymmetric PCR-SSCP and one insert ''T'' mutation and one G/A mutation in this region were found. The G/A mutation created a HhaI restriction enzyme digestion position and the frequencies studied by asymmetric PCR-SSCP and HhaI-RFLP in 116 Luxi cattle and 75 Holstein cows were all the same. The asymmetric PCR-SSCP had fewer, clearer and more stabile bands than traditional PCR-SSCP. This indicates that the asymmetric PCR-SSCP is suited for mutation detection.

Single nucleotide polymorphism rs7908486 of the TCF7L2 gene is highly associated with obesity in the Iraqi population

“…The body mass index (BMI) was calculated using the formula BMI= weight (kg)/height 2 . The BMI of normal weight, obesity and morbid obesity were defined as 18.5-24.9 kg/m 2 , 30-39.9 kg/m 2 , and > 40 kg/m 2 , respectively [14]. The waist circumference was measured at the narrowest point of the torso widthwise, usually just above the belly button, which is ≤102 cm in males and ≤88 cm in females [15].…”