The application of 57Fe Mössbauer spectroscopy in the investigation of iron uptake and translocation in plants

Kilcoyne, S.H.; Bentley, Phillip M.; Thongbai, Pongmanee; Gordon, D; Goodman, B. A.

doi:10.1016/s0168-583x(99)00579-0

Cited by 34 publications

(24 citation statements)

References 17 publications

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“…Compound C has similar parameters to those obtained by Kilcoyne et al (2000) for one compound found in roots and leaves of rice plants. These authors reported the presence of two major compounds in rice plants and considered the parameters consistent with Fe being in the form of oxyhydroxide or hydrated oxide species, suggesting similarity to ferrihydrite and lepidocrocite.…”

Section: Resultssupporting

confidence: 70%

“…isomer shift values reported by those authors are higher than the ones found in our work and by Kilcoyne et al (2000). It remains to be elucidated the identity of such a prevalent Fe form from rice plants.…”

Section: Resultscontrasting

confidence: 68%

“…Mössbauer parameters for ferrihydrite have been reported in studies with plants, such as pea roots and seeds, Canavalia ensiformis, rice and Imperata cylindrica (Goodman and DeKock, 1982;Yariv et al, 1988;Wade et al, 1993;Kilcoyne et al, 2000;Rodriguez et al, 2005). Ferrihydrite is the inorganic compound usually associated with Fe accumulation in ferritin (Chasteen and Harrison, 1999).…”

Section: Resultsmentioning

confidence: 97%

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Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

Silveira

Oliveira

Sperotto

et al. 2007

Braz. J. Plant Physiol.

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Iron is an essential nutrient for plants. In aerobic conditions, Fe is highly unavailable for plant uptake, and Fe deficiency can be severe in plants grown in calcareous soils. In waterlogged soils, however, Fe availability increases and can reach toxic concentrations. Rice is an important staple crop worldwide and faces iron deficiency or excess, depending on the growth conditions. To contribute to the study of mechanisms involved in response to Fe deficiency and resistance to Fe excess, experiments were carried out with rice cultivars BR-IRGA 409 (I409, susceptible to Fe toxicity) and EPAGRI 108 (E108, resistant to Fe toxicity) grown in culture solutions and submitted to Fe excess, control concentration or deficiency (500, 6.5 or zero mg L-1 Fe, respectively). Analysis of shoot dry weight confirmed the resistance of E108 plants to Fe excess. Mössbauer spectroscopy analysis indicated the presence of four different Fe3+compounds. The parameters obtained match those expected for ferrihydrite, lepidocrocite (and/or citrate) and Fe-nicotianamine. Mineral concentrations were determined using the PIXE (Particle Induced X-Ray Emission) technique. E108 plants had lower Fe concentrations than I409 plants when exposed to excess Fe. Except for lower Mn levels in roots and shoots, the excess of Fe did not result in lower nutrient concentrations in the susceptible cultivar compared to the resistant one. I409 plants seem to be affected directly by Fe toxicity rather than by secondary effects on mineral nutrition, whereas E108 plants seem to make use of the avoidance mechanism in the resistance to Fe overload. Both cultivars responded to Fe deficiency with allocation of P from roots to shoots. In addition to being more resistant to iron overload, E108 plants seem to be more efficient in inducing Fe deficiency responses.

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Section: Resultssupporting

confidence: 70%

Section: Resultscontrasting

confidence: 68%

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

Silveira

Oliveira

Sperotto

et al. 2007

Braz. J. Plant Physiol.

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show abstract

“…Besides, most of the iron was identified as ferritin, though a small fraction (\15%) present in complexes could not be excluded. A detailed study of iron uptake in rice (Kilcoyne et al 2000) showed mainly different Fe III -oxide components precipitated in the root cell walls. The spectra were also found to be characteristic of Fe III in ferritin and other complexed species that could not be further identified by the authors.…”

Section: Introductionmentioning

confidence: 99%

Investigation of iron pools in cucumber roots by Mössbauer spectroscopy: direct evidence for the Strategy I iron uptake mechanism

Kovács

Кузманн

Tatár

et al. 2008

Planta

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Distinct chemical species of iron were investigated by Mössbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. Mössbauer spectra of iron deficient roots supplied with 10-500 microM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The Mössbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No FeII was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of FeII-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.

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“…Anteriormente, se habían realizado estudios empleando isótopos radioactivos, pero la peligrosidad de los mismos dificultaba el manejo del material y el análisis de las muestras (Cesco et al, 2002;Bocanegra et al, 2006). También se ha utilizado la espectroscopía Mössbauer para detectar isótopos estables, pero esta técnica exige un alto enriquecimiento de los tejidos vegetales, difícil de conseguir en los ensayos de nutrición comunes y sobre todo en aquellos que requieren un corto tiempo de marcado (Kilcoyne et al, 2000;Kovács et al, 2005).…”

Section: Discussionunclassified

Respuestas Del Sistema De Absorción De Hierro en Las Raíces De Los Cítricos Ante Diferentes Condiciones Clorosantes Del Medio

Cuenca¹,

Rus²

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INFORMAN que,Dª Mary-Rus Martínez Cuenca, Ingeniera Agrónoma, ha realizado bajo nuestra dirección el trabajo que, con el título "Respuestas del sistema de absorción de hierro en las raíces de los cítricos ante diferentes condiciones clorosantes del medio", presenta para optar al grado de Doctora Ingeniera Agrónoma.Para que así conste a los efectos oportunos, firman el presente documento en Moncada a 28 de septiembre de 2012. Fdo. D. Francisco Legaz ParedesFdo. D. Eduardo Primo Millo RESUMENLa clorosis férrica es uno de los estreses abióticos más importantes para las plantas, y como tal, resulta un problema agrícola muy extendido especialmente para aquellos cultivos que se desarrollan en terrenos calizos, como es el caso de los cítricos en la Comunidad Valenciana.El objetivo de este trabajo fue caracterizar el sistema de absorción de hierro en los cítricos y su respuesta ante distintas situaciones clorosantes que comprometen la disponibilidad del elemento, provocando la deficiencia deFe en la planta. Los componentes del sistema de absorción del Feacidificación del medio, reducción del Fe(III), transporte a través de la membrana y alteración de la síntesis de ácidos orgánicos-, se analizaron a nivel fisiológico, bioquímico y molecular, en condiciones de ausencia de Fe externo, presencia de bicarbonato (HCO 3 -) y de otros micronutrientes como zinc (Zn) y manganeso (Mn). A més a més, s'aprecia que totes les respostes a la deficiència de Fe dels arrels estan metabòlicament relacionades les unes amb les altres i collaboren en l'absorció d'este element per la planta mitjançant la solubilització del Fe amb l'acidificació de la rizosfera, la reducció del ió Fe 3+ a Fe 2+ (més absorbible per la planta), i l'activació dels transportadors de ferro de la membrana plàsmica del a rrel. El nivell de tolerància dels cítrics a la clorosi fèrrica està determinat pel grau de resposta dels components del sistema d'absorció de Fe davant d'una situació clorosant, on el paper de l'enzim FC-R és especialment important. ABSTRACTIron chlorosis is one of the main abiotic stress factors in plants, and as such, is a widespread agricultural problem especially affecting those crops grown on calcareous soils, as in the case of citrus in the Valencia region.The aim of this study was to characterize the iron uptake system in

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The application of 57Fe Mössbauer spectroscopy in the investigation of iron uptake and translocation in plants

Cited by 34 publications

References 17 publications

Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

Investigation of iron pools in cucumber roots by Mössbauer spectroscopy: direct evidence for the Strategy I iron uptake mechanism

Respuestas Del Sistema De Absorción De Hierro en Las Raíces De Los Cítricos Ante Diferentes Condiciones Clorosantes Del Medio

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