The apoptotic pathways in the curcumin analog MHMD-induced lung cancer cell death and the essential role of actin polymerization during apoptosis

Zhou, Guang‐Zhou; Cao, Fakun; Du, Shiwei

doi:10.1016/j.biopha.2015.02.025

Cited by 29 publications

(18 citation statements)

References 47 publications

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“…Binding of nutraceuticals to actin at other sites is one potential mechanism that can limit actin polymerization, thus preventing cancer cell movement and cancer invasion. So far, little progress has been made in the search for compounds that disrupt the organization of actin filaments, although it is already known that cytoskeletal reorganization caused by the binding of ligands to actin allows not only the inhibition of migration but also apoptosis of cancer cells [ 8 , 9 , 10 ]. By binding to actin, nutraceuticals can modify and determine the intracellular location of transcription factors that play important roles in gene transcription [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

2018

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Actin functions are crucial for the ability of the cell to execute dynamic cytoskeleton reorganization and movement. Nutraceuticals that form complexes with actin and reduce its polymerization can be used in cancer therapy to prevent cell migration and metastasis of tumors. The aim of this study was to evaluate the ability of isoflavones to form complexes with actin. Docking simulation and isothermal titration calorimetry were used for this purpose. The formation of complexes by hydrogen bonds, hydrophobic and π-π interactions was demonstrated. Interactions occurred at the ATP binding site, which may limit the rotation of the actin molecule observed during polymerization and also at the site responsible for contacts during polymerization, reducing the ability of the molecule to form filaments. The greatest therapeutic potential was demonstrated by isoflavones occurring in red clover sprouts, i.e., biochanin A and formononetin, being methoxy derivatives of genistein and daidzein.

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Section: Introductionmentioning

confidence: 99%

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

2018

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“…A549 cells (3x10 5 cells/ml) were seeded into a 30-mm cell culture dish and treated with oxymatrine (1 mM) for 24, 48 and 72 h, respectively. The A549 cells were fixed (70% ethanol) for 2 h at 4˚C, washed with PBS, permeabilized (0.1% Triton X-100) and stained (PI-FITC) in accordance with the procedure described in Zhou et al (17). Then, cell samples were employed for the flow cytometry assay.…”

Section: Methodsmentioning

confidence: 99%

“…Wound healing assay. The migratory activity of A549 cells was determined using a wound healing assay, as previously described (17). Briefly, one pipette tip was used to make a scratch wound across each well in a 12-well plate.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have suggested that numerous natural extracts and chemically synthesized compounds exhibit therapeutic efficacy as potential candidates (13,14). The authors previously identified several curcumin analogs with anti-proliferative effects on A549 lung cancer cells and studied their molecular mechanisms of apoptosis, induced by the MHMD [(1E,6E)-4-(furan2yl) methylene]-1,7-bis(4-hydroxy-3-methoxyphe-nyl) hepta-1, 6 -diene-3, 5-dione), I HCH(2E,6E-2-(1H-indol-3-yl) methylene)-6-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone) and HBC (hydrazinobenzoylcurcumin), which represent possible therapeutic agents (15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

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Oxymatrine induces A549 human non‑small lung cancer cell apoptosis via extrinsic and intrinsic pathways

et al. 2017

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Oxymatrine is one of the primary natural compounds extracted from the Sophora flavescens, and has been reported to exhibit numerous pharmacological properties including cancer‑preventive and anti‑cancer effects, however the mechanisms as to how oxymatrine exhibits anti‑proliferative activity in non‑small cell lung carcinoma cells remains uncertain. The present study aimed to explore the mechanism of its anti‑cancer effect, and whether it is due to apoptosis induction and anti‑migration in the A549 lung cancer cell line. Detection of morphological alterations, MTT analysis, Hoechst/propidium iodide dual staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assays verified that oxymatrine induced A549 cell apoptosis. The caspase pan‑inhibitor z‑VAD‑FMK resulted in disappearance of oxymatrine‑elicited nuclei fragmentation via Hoechst 33342 staining. JC‑1 staining demonstrated a decrease in mitochondrial membrane potential which further verified the induction of apoptosis by oxymatrine. The caspase‑3, 8 and 9 activities of oxymatrine‑treated cells were activated, which suggested that extrinsic and intrinsic apoptotic pathways were involved in the anti‑proliferative effects of oxymatrine in A549 cells. Furthermore, the wound healing assay verified the anti‑migratory effects of oxymatrine in A549 cells.

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“…22 Recently, several studies have reported the effects of some nanomaterials on actin cytoskeleton in cancer cells. For example, carbon nanomaterial, 23 curcumin analog MHMD, 24 grapheneoxide nanosheets, 25 AgNPs, 26 and ZnONPs 27 affected F-actin cytoskeleton through targeting or disruption of F-actin. It has been reported the metal-organic frameworks (IRMOF-3) possesses the ability to disrupt F-actin and tubulin, blocking the rat pheochromocytoma cell division.…”

Section: Introductionmentioning

confidence: 99%

Selective Mediation of Ovarian Cancer Cell Death by Pristine Carbon Quantum Dots/Cu2O Composite Through Targeting Matrix Metalloproteinases, Angiogenic Cytokines and Cytoskeleton

Chen

Nie

et al. 2020

Preprint

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It was shown that some nanomaterials may have anticancer properties, but lack of selectivity is one of challenges, let alone selective suppression of cancer growth by regulating the cellular microenvironment. Herein, we demonstrated for the first time that carbon quantum dots/Cu2O composite (CQDs/Cu2O) selectively inhibited ovarian cancer SKOV3 cells by targeting cellular microenvironment, such as matrix metalloproteinases, angiogenic cytokines and cytoskeleton. The result was showed CQDs/Cu2O possessed anticancer properties against SKOV3 cells with IC50 = 0.85 μg mL−1, which was approximately 3-fold lower than that in normal mouse embryonic fibroblasts BABL-3T3 cells. Compared with popular anticancer drugs, the IC50 of CQDs/Cu2O was approximately 114-fold and 75-fold lower than the IC50 of commercial artesunate (ART) and oxaliplatin (OXA). Furthermore, CQDs/Cu2O possessed the ability to decrease the expression of MMP-2/9 and induced alterations in the cytoskeleton of SKOV3 cells by disruption of F-actin. It also exhibited stronger antiangiogenic effects than commercial antiangiogenic inhibitor (SU5416) through down-regulating the expression of VEGFR2. In addition, CQDs/Cu2O has a vital function on transcriptional regulation of multiple genes in SKOV3 cells, where 495 genes were up-regulated and 756 genes were down-regulated. It is worth noting that CQDs/Cu2O also regulated angiogenesis-related genes in SKOV3 cells, such as Maspin and TSP1 gene, to suppress angiogenesis. Therefore, CQDs/Cu2O selectively mediated of ovarian cancer cells death mainly through decreasing the expression of MMP-2, MMP-9, F-actin, and VEGFR2, meanwhile CQDs/Cu2O caused apoptosis of SKOV3 via S phase cell cycle arrest. These findings reveal a new application for the use of CQDs/Cu2O composite as potential therapeutic interventions in ovarian cancer.

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The apoptotic pathways in the curcumin analog MHMD-induced lung cancer cell death and the essential role of actin polymerization during apoptosis

Cited by 29 publications

References 47 publications

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

Oxymatrine induces A549 human non‑small lung cancer cell apoptosis via extrinsic and intrinsic pathways

Selective Mediation of Ovarian Cancer Cell Death by Pristine Carbon Quantum Dots/Cu2O Composite Through Targeting Matrix Metalloproteinases, Angiogenic Cytokines and Cytoskeleton

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