The apicoplast: now you see it, now you don’t

McFadden, Geoffrey I.; Yeh, Ellen

doi:10.1016/j.ijpara.2016.08.005

Cited by 107 publications

(92 citation statements)

References 136 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Plasmodium apicoplast is a known site of several anabolic processes including biosynthesis of type II fatty acids (FASII) . Several enzymes involved in its biosynthetic processes are NADPH‐dependent, rendering the NADPH concentration potentially rate limiting.…”

Section: Resultsmentioning

confidence: 99%

“…This is consistent with the successful transmission of NTHDPP sporozoites upon crossing with LAP3/mCherry (Fig 3E and G). Nonetheless, NTH/ND500LK sporozoites were significantly less effective (P = 0.0029, Fisher) than their NTH/GFP counterparts in establishing blood-stage infections ( The Plasmodium apicoplast is a known site of several anabolic processes including biosynthesis of type II fatty acids (FASII) [38,39]. Several enzymes involved in its biosynthetic processes are NADPH-dependent, rendering the NADPH concentration potentially rate limiting.…”

Section: Nth Contributes To the Transition From Sporozoite To Intraermentioning

confidence: 99%

See 1 more Smart Citation

NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission

et al. 2020

View full text Add to dashboard Cite

Nicotinamide adenine dinucleotide (NAD) and its phosphorylated form (NADP) are vital for cell function in all organisms and form cofactors to a host of enzymes in catabolic and anabolic processes. NAD(P) transhydrogenases (NTHs) catalyse hydride ion transfer between NAD(H) and NADP(H). Membrane‐bound NTH isoforms reside in the cytoplasmic membrane of bacteria, and the inner membrane of mitochondria in metazoans, where they generate NADPH. Here, we show that malaria parasites encode a single membrane‐bound NTH that localises to the crystalloid, an organelle required for sporozoite transmission from mosquitos to vertebrates. We demonstrate that NTH has an essential structural role in crystalloid biogenesis, whilst its enzymatic activity is required for sporozoite development. This pinpoints an essential function in sporogony to the activity of a single crystalloid protein. Its additional presence in the apicoplast of sporozoites identifies NTH as a likely supplier of NADPH for this organelle during liver infection. Our findings reveal that Plasmodium species have co‐opted NTH to a variety of non‐mitochondrial organelles to provide a critical source of NADPH reducing power.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Nth Contributes To the Transition From Sporozoite To Intraermentioning

confidence: 99%

NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission

et al. 2020

View full text Add to dashboard Cite

show abstract

“…www.molinf.com intervention. [26] A combination of imanixil and tannin or encalaret at optimal dose could be tested as a potential anti-malarial drug without overt adverse effects. [27]…”

Section: Full Papermentioning

confidence: 99%

Proteomic Atomics Reveals a Distinctive Uracil‐5‐Methyltransferase

Pramanik

Thaker

Perumal

et al. 2020

Molecular Informatics

View full text Add to dashboard Cite

Carbon (C), hydrogen (H), nitrogen (N), oxygen (O), and sulfur (S) atoms intrigue as they are the foundation for amino acid (AA) composition and the folding and functions of proteins and thus define and control the survival of a cell, the smallest unit of life. Here, we calculated the proteomic atom distribution in > 1500 randomly selected species across the entire current phylogenetic tree and identified uracil-5-methyltransferase (U5MTase) of the protozoan parasite Plasmodium falciparum (Pf, strain Pf3D7), with a distinct atom and AA distribution pattern. We determined its apicoplast location and in silico 3D protein structure to refocus attention exclusively on U5MTase with tremendous potential for therapeutic intervention in malaria. Around 300 million clinical cases of malaria occur each year in tropical and subtropical regions of the world, resulting in over one million deaths annually, placing malaria among the most serious infectious diseases. Genomic and proteomic research of the clades of parasites containing Pf is progressing slowly and the functions of most of the~5300 genes are still unknown. We applied a 'bottom-up' comparative proteomic atomics analysis across the phylogenetic tree to visualize a protein molecule on its actual basis -i. e., its atomic level. We identified a protruding Pf3D7-specific U5MTase, determined its 3D protein structure, and identified potential inhibitory drug molecules through in silico drug screening that might serve as possible remedies for the treatment of malaria. Besides, this atomic-based proteome map provides a unique approach for the identification of parasite-specific proteins that could be considered as novel therapeutic targets.

show abstract

“…Plasmodium gehört aber wie der Mensch zu den Eukaryoten. Es dauerte fast 50 Jahre, bis der Effekt von Antibiotika auf den Parasiten verstanden wurde: Malariaparasiten besitzen neben dem Mitochondrium ein weiteres Organell, das durch Endosymbiose entstand, den Apikoplasten . Seinen Ursprung hatte der Apikoplast als freilebender Prokaryot, wahrscheinlich als Cyanobakterium, welches in der Lage war, Photosynthese zu betreiben.…”

Section: Da Steckt Eine Pflanze Drinunclassified

Das ungewöhnliche Leben der Malariaparasiten

Przyborski¹,

Ganter

2018

Biologie in unserer Zeit

View full text Add to dashboard Cite

Zusammenfassung Malaria ist die wichtigste Infektionskrankheit, die von Krankheitsüberträgern (Stechmücken der Gattung Anopheles) verbreitet wird. Sich ausbreitende Resistenzen gegen Chemotherapeutika bedrohen die Erfolge der Malariabekämpfung der letzten Jahre. Ein umfangreiches Verständnis der ungewöhnlichen Biologie des Parasiten ist entscheidend, um dessen Schwachpunkte zu erkennen und für dringend benötigte neue Interventionsstrategien auszunutzen.

show abstract

The apicoplast: now you see it, now you don’t

Cited by 107 publications

References 136 publications

NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission

NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission

Proteomic Atomics Reveals a Distinctive Uracil‐5‐Methyltransferase

Das ungewöhnliche Leben der Malariaparasiten

Contact Info

Product

Resources

About