The API ZYM system in the identification of Gram-negative anaerobes.

Tharagonnet, Danka; Sisson, P. R.; Roxby, C. M.; Ingham, H. R.; Selkon, J. B.

doi:10.1136/jcp.30.6.505

Cited by 70 publications

(29 citation statements)

References 12 publications

(8 reference statements)

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“…Our findings in this regard are generally consistent with reports of others (4,6,7). Except for enzyme-substrate reactions in microcupule numbers 2, 5, 6, 8, 10 and 17, reproducibilities of the remaining reactions were >~ 99 %.…”

Section: Variabilitysupporting

confidence: 94%

Rapid enzymatic characterization of clinically encountered anaerobic bacteria with the API ZYM system

Marler

Allen

Siders

1984

Eur. J, Clin. Microbiol.

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The purpose of this study was to determine if enzyme profiles of anaerobic bacteria, obtained with the API ZYM system, are sufficiently distinctive and reproducible to merit future development of the system and an expanded data base for the four major groups of clinically encountered anaerobes. Of the total 155 clinical isolates and reference strains that were tested, 88% had distinctive patterns. It was possible to differentiate between 89% of the anaerobic gram-negative bacilli, 64% of anaerobic cocci, 100% of anaerobic gram-positive non-sporeforming bacilli and 100% of the Clostridium spp. using only the API ZYM plus Gram reaction, morphology and relation to oxygen. Overall reproducibility for 1,330 enzyme-substrate reactions was 97%. It was concluded that the API ZYM could be a practical system for rapid identification of clinical anaerobe isolates provided that an expanded data base is developed and that certain additional recommended tests are used.

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Section: Variabilitysupporting

confidence: 94%

Rapid enzymatic characterization of clinically encountered anaerobic bacteria with the API ZYM system

Marler

Allen

Siders

1984

Eur. J, Clin. Microbiol.

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“…'s blood culture and de tected by the API ZYM system are denoted by an asterisk in table I. These results are in agreement with recently published data [14] for this species.…”

Section: Api Zym Profile Of Bacterial Enzymessupporting

confidence: 82%

Tk Polyagglutination Associated with Reduced A and H Activity

Bird¹,

Mitchell²,

Wingham³

et al. 1978

Vox Sanguinis

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“…In the present study, use also has been made of API ZYM, a commercially available micro-method which permits a rapid, simultaneous assay of 19 enzymes. The test system has been applied to a wide range of biological materials and preliminary studies (Tharagonnet et al 1977;Cohen el al. 1980;Hofstad 1980) suggest a possible role in identifying anaerobic bacteria.…”

mentioning

confidence: 99%

A Study of Rapid and Simplified Confirmatory Tests for Clostridium perfringens

Mead¹,

León

Adams³

1981

Journal of Applied Bacteriology

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Strains of Clostridium perfringens and culturally similar species which also may grow on selective isolation media for this organism were examined by conventional confirmatory tests, the API ZYM system and by individual tests for phosphatase and glutamic acid decarboxylase activity. API ZYM tests, involving 19 different enzymes, confirmed the known similarity between Cl. perfringens, Cl. absonum, Cl. paraperfringens and Cl. sardiniensis but effectively distinguished this group from Cl. bifermentans, Cl. celatum, Cl. perenne and Cl. sordellii. A similar separation was achieved by a single test for acid phosphatase which could be applied to individual colonies on a plating medium. Because the acid phosphatase test was found to be of greater value than nitrate reduction in distinguishing Cl. perfringens, it could replace the latter in the usual series of confirmatory tests. It is suggested that strains from Cl. perfringens isolation media should be screened for acid phosphatase activity at the purification stage and only positive strains subjected to further tests. It was found that Cl. perfringens could not be distinguished from the other species on the basis of glutamate decarboxylase activity.

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The API ZYM system in the identification of Gram-negative anaerobes.

Cited by 70 publications

References 12 publications

Rapid enzymatic characterization of clinically encountered anaerobic bacteria with the API ZYM system

Rapid enzymatic characterization of clinically encountered anaerobic bacteria with the API ZYM system

Tk Polyagglutination Associated with Reduced A and H Activity

A Study of Rapid and Simplified Confirmatory Tests for Clostridium perfringens

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