The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis

Ren, Shuting; Liao, Yue‐Yuan; Kang, Xiaoning; Li, Yiping; Zhang, Hui; Ai, Hong; Sun, Qiang; Jing, Jing; Zhao, Xiao; Tan, Lifang; Shen, Xin-Liang; Wang, Bing

doi:10.1007/s11095-013-0996-5

Cited by 24 publications

(12 citation statements)

References 41 publications

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“…Moreover, the energy lose for low frequency ultrasound is low and hence deeper tissue can be treated. 8,9 In addition, our findings also demonstrated that these treatments would not damage the normal function of prostatic tissue, with no significant difference in renal function laboratory data (BUN, Cr, AST, and ALT).…”

Section: Discussionsupporting

confidence: 60%

Combined Low-Frequency Ultrasound and Microbubble Contrast Agent for the Treatment of Benign Prostatic Hyperplasia

Yang

Tang²,

Bai³

et al. 2013

Journal of Endourology

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Background and Purpose: Benign prostatic hyperplasia is one of the most common conditions in middle-aged and elderly men. The aim of the study was to investigate the treatment effects of low-frequency ultrasound combined with a microbubbles agent on benign prostatic hyperplasia. Methods: Eighteen 7-year-old male beagles with prostatic hyperplasia were randomly divided into six groups (n = 3): Control group, 21 kHz ultrasound group, 21 kHz ultrasound and microbubble contrast agent group, 1 MHz ultrasound group, 1 MHz ultrasound and microbubble contrast agent group, and microbubble contrast agent group. The histopathologic damage to prostate cells was assessed via transmission electron microscopy and optical microscopy. The protein expressions of prostate-specific antigen (PSA), inducible nitric oxide synthase (iNOS), and super oxidase dimutase (SOD) were detected by enzyme-linked immunosorbent assay. Levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine (Cr) were detected by the Biochemistry Automatic Analyzer. Results: Significant tissue injury, mitochondria injury, and cell apoptosis were observed in 21 kHz ultrasound and the microbubble contrast agent group. Compared with the control and microbubbles contrast agent groups, the decrease in levels of PSA or increase in levels of iNOs and SOD in the other four groups were statistically significant (P < 0.05). The lowest level of PSA and the highest levels of iNOs and SOD were observed in the 21 kHz ultrasound and microbubbles contrast agent group. No significant changes in levels of AST, ALT, BUN, and Cr were observed between the six groups. Conclusions: Our results suggest that lower frequency ultrasound may have a better effect on benign prostatic hyperplasia, and microbubble contrast agent application further strengthens this biological effect.

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Section: Discussionsupporting

confidence: 60%

Combined Low-Frequency Ultrasound and Microbubble Contrast Agent for the Treatment of Benign Prostatic Hyperplasia

Yang

Tang²,

Bai³

et al. 2013

Journal of Endourology

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“…Nano-scale MBs usually have the diameter within 1 lm. Under a certain acoustical condition, MBs physically interact with some target tissues and increase temporarily the drug uptake by cells with improved cell permeability [12][13][14]. Nevertheless, MB release of drugs and the drug uptake by cells hardly appear in tissues without ultrasound exposure.…”

Section: Introductionmentioning

confidence: 99%

“…J Drug Target, Early Online:[1][2][3][4][5][6][7][8][9][10][11][12][13] Journal of Drug Targeting Downloaded from informahealthcare.com by University of Otago on 07/28/15For personal use only.…”

mentioning

confidence: 99%

Inhibitory effect of epirubicin-loaded lipid microbubbles with conjugated anti-ABCG2 antibody combined with therapeutic ultrasound on multiple myeloma cancer stem cells

Shi

Yang

et al. 2015

Journal of Drug Targeting

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Ultrasound-targeted microbubble destruction (UTMD) technique is thought to improve the chemotherapeutic agent delivery from microbubbles (MBs) in tumor tissues and reduce the side effects in non-tumor tissues. Multiple myeloma (MM) is a bone marrow cancer and remains to be an incurable disease. In this study, we used the UTMD technique to investigate the inhibitory effect of our developed novel reagent on MM cancer stem cells (CD138(-)CD34(-)MM CSCs) that are MM cells with CD138(-)CD34(-) phenotypes, responsible for MM-initiating potential, drug resistance and eventual relapse. The preparatory steps of novel reagent was first epirubicin (EPI)-loaded in the lipid MBs that was consisted of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]-biotin, dipalmitoyl-phosphatidylglycerol and 25-NBD-cholesterol, then anti-ABCG2 monoclonal antibody (mAb) was conjugated onto the MB surface to form EPI-MBs+mAb. CD138(-)CD34(-)MM CSCs were isolated from human MM RPMI 8226 cell line by the magnetic associated cell sorting method. The results showed that the attenuated proliferation, migration and invasion ability, and increased apoptosis were observed when MM CSCs were incubated with a various agents. EPI-MBs+mAb combined with therapeutic ultrasound significantly promoted the MM CSC apoptosis compared with EPI, EPI-MBs alone or EPI-MBs+mAb without ultrasound exposure. These results suggest that the developed EPI-MBs+mAb combined with therapeutic ultrasound remarkably induced MM CSC apoptosis in vitro.

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“…Secondly, low frequency ultrasound can induce cell membrane sonoporation and permeabilize the biological barrier, facilitating the transport of macromolecules and gene delivery [6,7]. Importantly, low frequency ultrasound has direct antitumor effect in vitro experiments, especially when combined with microbubbles [8,9]. Thus, low frequency ultrasound has prominent advantages in treatment of castration-resistant prostate cancer [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, low frequency ultrasound has direct antitumor effect in vitro experiments, especially when combined with microbubbles [8,9]. Thus, low frequency ultrasound has prominent advantages in treatment of castration-resistant prostate cancer [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Caveolin-1 as a biomarker to predict therapeutic effect of low-frequency ultrasound combined with SonoVue on prostate cancer in nude mice model

Nan

Bai

et al. 2014

CBM

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The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis

Abstract: The self-made DOC + MBs have potential as a new ultrasound contrast agent and drug-loaded microbubble, and can obviously enhance the antitumor effect of DOC under LFUS exposure.

Cited by 24 publications

References 41 publications

Combined Low-Frequency Ultrasound and Microbubble Contrast Agent for the Treatment of Benign Prostatic Hyperplasia

Combined Low-Frequency Ultrasound and Microbubble Contrast Agent for the Treatment of Benign Prostatic Hyperplasia

Inhibitory effect of epirubicin-loaded lipid microbubbles with conjugated anti-ABCG2 antibody combined with therapeutic ultrasound on multiple myeloma cancer stem cells

Caveolin-1 as a biomarker to predict therapeutic effect of low-frequency ultrasound combined with SonoVue on prostate cancer in nude mice model

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