The antithrombotic effect of RSNK in blood-stasis model rats

Dang, Xuan; Miao, Jingjing; Chen, Anqi; Chen, Lin; Liang, Jinru; Xie, Ruiqin; Zhao, Ye

doi:10.1016/j.jep.2015.06.030

Cited by 54 publications

(39 citation statements)

References 16 publications

(16 reference statements)

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“…TXA2 and PGI2, metabolites of arachidonic acid, play an important role in thrombosis. The pathological imbalance of TXA2 and PGI2 may stimulate platelet aggregation, vasospasm and thrombosis, which can lead to angina pectoris, myocardial infarction and cerebrovascular accident (Dang et al, ). TXA2, a product of arachidonic acid, can accelerate platelet aggregation, vasospasm and thrombosis (Li, Li, et al, ).…”

Section: Resultsmentioning

confidence: 99%

Effects of Tao‐Hong‐Si‐Wu decoction on acute blood stasis in rats based on a LC‐Q/TOF‐MS metabolomics and network approach

Hua

et al. 2017

Biomedical Chromatography

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A novel approach using metabolomics coupled with a metabolic network was used to investigate the effects of Tao-Hong-Si-Wu decoction (THSWD) on the rat model of acute blood stasis syndrome. Acute blood stasis syndrome was induced by placing the rats in ice-cold water following two injections with epinephrine. The hemorheological indicators [whole blood viscosity (WBV) and plasma viscosity (PV)] and the blood coagulation indicators [thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen (FIB)] were detected. The nonparametric univariate method and multivariate statistical analysis were performed for determining the potential biomarkers. A correlation map was structured between biochemical indicators and hub metabolites to explain the effects mechanism of THSWD. After the administration of THSWD, the levels of WBV, PV, TT, APTT and FIB returned to levels observed in the control group. According to metabolomics coupled with metabolic network analysis, the intervention of THSWD in rats with acute blood stasis syndrome induced substantial and characteristic changes in their metabolic profiles. Fifteen metabolites were screened, which mainly involved 10 pathways and five hub metabolites, namely, l-glutamate, l-phenylalanine, N-acylsphingosine, arachidonic acid and phosphatidate. The biochemical indicators and hub metabolites could be adjusted to close to normal levels by THSWD. Therefore, combining metabolomics and metabolic network helped to evaluate the effects of THSWD on acute blood stasis.

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Section: Resultsmentioning

confidence: 99%

Effects of Tao‐Hong‐Si‐Wu decoction on acute blood stasis in rats based on a LC‐Q/TOF‐MS metabolomics and network approach

Hua

et al. 2017

Biomedical Chromatography

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“…TXA 2, a metabolite of arachidonic acid, is well known to regulate platelet aggregation and vasomotor response along with nitric oxide [22]. Endothelial cells secrete nitric oxide which inhibits clot formation through vasodilation, by inhibiting the production of TXA 2 [23].…”

Section: Discussionmentioning

confidence: 99%

“…PT is related to the extrinsic clotting pathway and aPTT is used to assess the intrinsic coagulation phase in plasma [22]. These factors can serve as important risk factors in cardiovascular disease and as independent indicators of the progression of atherosclerosis [25].…”

Section: Discussionmentioning

confidence: 99%

The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet

Kim

Lee

Jung

et al. 2017

Journal of Ginseng Research

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BackgroundThe present study investigated the effect of ginseng berry hot water extract (GBx) on blood flow via the regulation of lipid metabolites and blood coagulation in rats fed a high-fat diet (HFD).MethodsSixty rats were divided into five groups in descending order of body weight. Except for the control group, the other four groups were fed a HFD containing 45% kcal from fat for 11 wk without GBx. GBx groups were then additionally treated by gastric gavage with GBx dissolved in distilled water at 50 (GBx 50) mg/kg, 100 (GBx 100) mg/kg, or 150 (GBx 150) mg/kg body weight for 6 wk along with the HFD. To investigate the effects of GBx on rats fed a HFD, biochemical metabolite, blood coagulation assay, and histological analysis were performed.ResultsIn the experiments to measure the serum levels of leptin and apolipoprotein B/A, GBx treatment attenuated the HFD-induced increases in these metabolites (p < 0.05). Adiponectin and apolipoprotein E levels in GBx-treated groups were significantly higher than the HFD group. Prothrombin time and activated partial thromboplastin time were increased in all GBx-treated groups. In the GBx-treated groups, the serum levels of thromboxane A2 and serotonin were decreased and concentrations of serum fibrinogen degradation products were increased (p < 0.05). Moreover, histomorphometric dyslipidemia-related atherosclerotic changes were significantly improved by treatment with GBx.ConclusionThese results suggest the possibility that GBx can ameliorate blood flow by decreasing intima-media thickness via the regulation of blood coagulation factors related to lipid metabolites in rats fed a HFD.

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“…It acts by dilating blood vessels, improving cerebral (pial) microcirculation and blood flow [3], reducing blood viscosity, platelet aggregation, and microintravascular fibrin precipitation, inhibiting thrombus formation [4], and attenuating neuronal apoptosis [5]. Modern pharmacological research has shown that PUE inhibits inflammatory reactions [6], possesses antithrombotic [7], and hypoglycaemic [8] properties, and suppresses oxidative stress [9]. …”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA

et al. 2017

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Puerarin (PUE) is a compound isolated from the roots of Pueraria lobata. We studied the pharmacokinetics and tissue distribution kinetics of PUE in Sprague-Dawley rats following intraperitoneal administration of three concentrations. Indirect competitive ELISA based on an anti-PUE monoclonal antibody was used to determine the concentration of PUE in the blood, heart, liver, spleen, lung, kidney, hippocampus, cerebral cortex, and striatum. The plasma and tissue distribution kinetic characteristics following a single injection of PUE (20, 40 and 80 mg/kg) were calculated using a non-compartment model. In the high-dose (80 mg/kg) and medium-dose (40 mg/kg) groups, the kinetic profile of PUE in blood and kidney samples showed two absorption peaks, while that of the other tissues showed only one peak. In the low-dose (20 mg/kg) group, there was only one peak, irrespective of the sample type. Pharmacokinetic parameters, such as the area under the curve, Cmax, and Tmax varied according to the administered dose. AUC and Cmax values increased dose-dependently. PUE was widely distributed in areas of the brain such as the hippocampus, cerebral cortex, and striatum, providing a foundation for guiding the use of PUE in the treatment of cerebral ischaemic stroke and neurodegenerative diseases.

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The antithrombotic effect of RSNK in blood-stasis model rats

Cited by 54 publications

References 16 publications

Effects of Tao‐Hong‐Si‐Wu decoction on acute blood stasis in rats based on a LC‐Q/TOF‐MS metabolomics and network approach

Effects of Tao‐Hong‐Si‐Wu decoction on acute blood stasis in rats based on a LC‐Q/TOF‐MS metabolomics and network approach

The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet

Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA

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