The antiretroviral agents azidothymidine, stavudine, and didanosine have the identical mutational fingerprint in the rpoB region of Escherichia coli

Abstract

We looked at the mutational fingerprints of three antiretroviral (anti-HIV) agents, azidothymidine (AZT), stavudine (STAV), and didanosine (DIDA) in the rpoB system of Escherichia coli and compared them with each other and with the fingerprints of trimethoprim and of spontaneous mutations in a wild-type and a mutT background. All three agents gave virtually identical fingerprints in the wild-type background, causing only A: T!C:G changes at 3 of the 12 A:T!C:G possible sites among the total of 92 possible base substitution mutations, even though AZT and STAV are thymidine analogs but DIDA is an adenosine analog. As all three agents are reverse transcriptase inhibitors, and act as chain blockers, the common fingerprint may be a property of chain blocking agents.anti-HIV agents, bacterial systems, mutagenesis | INTRODUCTIONAzidothymidine (AZT; Zidovudine; 3 0 -azido-3 0 -deoxythymidine), stavudine (STAV; 2 0 ,3 0 -dideoxythymidine), and didanosine (DIDA; 2 0 ,3 0 -dideoxyinosine), depicted in Figure 1, are reverse transcriptase