The antiproliferative effects of cold atmospheric plasma‐activated media on different cancer cell lines, the implication of ozone as a possible underlying mechanism

Mokhtari, Hesam; Farahmand, Leila; Yaserian, Kiomars; Jalili, Neda; Majidzadeh‐A, Keivan

doi:10.1002/jcp.27428

Cited by 23 publications

(17 citation statements)

References 30 publications

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“…In the current study, we demonstrated that only a short time exposure of 0.5 h could exhibit sufficient anti-tumor effects in vitro on endometrial cancer cells. Previous reports revealed that longer treatments with direct exposure of NEAPP or PAM resulted in significantly lower viability in cancer cells 8,22 . In addition, Takeda et al revealed that not only adherent cells, but also floating cells, presented a decrease in adhesion capacity 19 .…”

Section: Discussionmentioning

confidence: 96%

Plasma-activated medium promotes autophagic cell death along with alteration of the mTOR pathway

Yoshikawa

Liu

Nakamura

et al. 2020

Sci Rep

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the biological function of non-thermal atmospheric pressure plasma has been widely accepted in several types of cancer. We previously developed plasma-activated medium (pAM) for clinical use, and demonstrated that PAM exhibits a metastasis-inhibitory effect on ovarian cancer through reduced MMP-9 secretion. However, the anti-tumor effects of PAM on endometrial cancer remain unknown. In this study, we investigated the inhibitory effect of PAM on endometrial cancer cell viability in vitro.Our results demonstrated that AMEC and HEC50 cell viabilities were reduced by PAM at a certain PAM ratio, and PAM treatment effectively increased autophagic cell death in a concentration dependent manner. in addition, we evaluated the molecular mechanism of pAM activity and found that the mtoR pathway was inactivated by pAM. Moreover, our results demonstrated that the autophagy inhibitor MHY1485 partially inhibited the autophagic cell death induced by PAM treatment. These findings indicate that pAM decreases the viability of endometrial cancer cells along with alteration of the mtoR pathway, which is critical for cancer cell viability. collectively, our data suggest that pAM inhibits cell viability while inducing autophagic cell death in endometrial cancer cells, representing a potential novel treatment for endometrial cancer.Endometrial cancer is the most common gynecologic malignancy in Japan, with an estimated 13,600 new cases diagnosed in 2015 1 . The incidence of new cases is believed to have increased over the last few decades due to increased levels of obesity, hypertension, and diabetes mellitus, particularly in South Africa and several Asian countries including Japan 1,2 . Although the majority of endometrial cancer patients are diagnosed in the early stage and outcomes are generally favorable because of the typical symptom of irregular vaginal bleeding in women of menopausal age, the prognoses of patients in advanced stages not amenable to localized therapies remain poor 1 . Indeed, a frequent pattern of progression and recurrence is peritoneal metastasis, which is often resistant to systemic chemotherapy 3 . In addition, cytotoxic agents for endometrial cancer are relatively limited compared with other gynecologic malignancies. Thus, there is an increasing demand for novel approaches to peritoneal dissemination.For the past decade, nonequilibrium atmospheric pressure plasma (NEAPP) has been studied for its clinical applications such as sterilization, wound healing, and cancer therapy 4-6 . NEAPP represents a partially ionized gas with energy mainly accumulated as free electrons 7 . Recent studies have revealed that in addition to the direct irradiation of NEAPP to bacteria or tissues, indirect plasma treatment with plasma-activated medium (PAM) and plasma-activated lactate (PAL), which are activated by NEAPP, exhibit anti-tumor activity in several types of cancer, including ovarian, pancreatic, colorectal and gastric cancers 4,8-13 . The molecular mechanism of the anti-tumor effects triggered by PAM have been investigat...

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Section: Discussionmentioning

confidence: 96%

Plasma-activated medium promotes autophagic cell death along with alteration of the mTOR pathway

Yoshikawa

Liu

Nakamura

et al. 2020

Sci Rep

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“…Plasma-exposed eukaryotic cells in vitro demonstrate the effects such as cell detachment, cell migration/proliferation alterations, apoptosis or necrosis, all depending on cell type and exposure parameters. 2,20,21 But to our knowledge, our study is the first to address plasma-induced SG formation in eukaryotic cells. For this reason, we established FlpIn-SH-SY5Y-mScarletI-G3BP1-Myc cell line with inducible G3BP1 protein expression, indicative of SG formation that can be easily monitored due to G3BP1 fusion to mScarletI red fluorescent protein.…”

Section: Discussionmentioning

confidence: 97%

“…This could possibly be explained by vigorous plasma mixing with air at lower flow rates and plasma flow needed to produce long-life versus short-life reactive species 1 that could underlie this differential effect. Due to the resemblance of plasma effects 4,7,21,32 to compounds 33 causing ROS production and oxidative stress, we characterized plasma induced SGs in comparison with the ones induced by arsenite. Our results are indicative of oxidative stress to play, but not to have the sole role in plasma induced SG formation.…”

Section: Biomaterials Science Papermentioning

confidence: 99%

“…Moreover, its addition to SG-containing cells can induce rapid disassembly of SG and liberation of mRNAs into an actively translating pool. 18,19 Though many effects of plasma have been demonstrated in in vitro studies, 2,20,21 none of these have addressed early stress response of the cells to plasma treatment, involving stress granule (SG) formation. It is hypothesized that plasma, similar to known oxidative stress insults such as arsenite, could trigger SG formation.…”

Section: Introductionmentioning

confidence: 99%

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Cold atmospheric plasma induces stress granule formation via an eIF2α-dependent pathway

et al. 2020

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“…PAM may be a promising tool in cancer treatment with the formation of O 3 as a probable mechanism, as was demonstrated by the analysis of cancer cell lines, of which human breast cancer cell line SKBR3 was most susceptible to PAM [266]. In vitro, and in vivo analysis also revealed that PAM could selectively trigger apoptosis, and hinder the proliferation and migration of triple-negative breast cancer compared to the other subtypes [55].…”

Section: Indirect Anti-tumour Effects Of Capmentioning

confidence: 91%

Cold Atmospheric Pressure Plasma (CAP) as a New Tool for the Management of Vulva Cancer and Vulvar Premalignant Lesions in Gynaecological Oncology

Žúbor

Wang

Líšková

et al. 2020

IJMS

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Vulvar cancer (VC) is a specific form of malignancy accounting for 5–6% of all gynaecologic malignancies. Although VC occurs most commonly in women after 60 years of age, disease incidence has risen progressively in premenopausal women in recent decades. VC demonstrates particular features requiring well-adapted therapeutic approaches to avoid potential treatment-related complications. Significant improvements in disease-free survival and overall survival rates for patients diagnosed with post-stage I disease have been achieved by implementing a combination therapy consisting of radical surgical resection, systemic chemotherapy and/or radiotherapy. Achieving local control remains challenging. However, mostly due to specific anatomical conditions, the need for comprehensive surgical reconstruction and frequent post-operative healing complications. Novel therapeutic tools better adapted to VC particularities are essential for improving individual outcomes. To this end, cold atmospheric plasma (CAP) treatment is a promising option for VC, and is particularly appropriate for the local treatment of dysplastic lesions, early intraepithelial cancer, and invasive tumours. In addition, CAP also helps reduce inflammatory complications and improve wound healing. The application of CAP may realise either directly or indirectly utilising nanoparticle technologies. CAP has demonstrated remarkable treatment benefits for several malignant conditions, and has created new medical fields, such as “plasma medicine” and “plasma oncology”. This article highlights the benefits of CAP for the treatment of VC, VC pre-stages, and postsurgical wound complications. There has not yet been a published report of CAP on vulvar cancer cells, and so this review summarises the progress made in gynaecological oncology and in other cancers, and promotes an important, understudied area for future research. The paradigm shift from reactive to predictive, preventive and personalised medical approaches in overall VC management is also considered.

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The antiproliferative effects of cold atmospheric plasma‐activated media on different cancer cell lines, the implication of ozone as a possible underlying mechanism

Cited by 23 publications

References 30 publications

Plasma-activated medium promotes autophagic cell death along with alteration of the mTOR pathway

Plasma-activated medium promotes autophagic cell death along with alteration of the mTOR pathway

Cold atmospheric plasma induces stress granule formation via an eIF2α-dependent pathway

Cold Atmospheric Pressure Plasma (CAP) as a New Tool for the Management of Vulva Cancer and Vulvar Premalignant Lesions in Gynaecological Oncology

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