The antioxidative effects of three lactobacilli on high-fat diet induced obese mice

Abstract

In this paper, three Lactobacillus strains (L. coryniformis subsp. torquens T3, L. paracasei subsp. paracasei M5 and L. paracaseisubp. paracasei X12) isolated by our laboratory were investigated for antioxidant activity in vitro and in vivo. Hydroxyl and 2,2-diphenyl-1picrylhydrazyl (DPPH) free radicals scavenging activities, and reducing power were examined in vitro. Three Lactobacillus strains were administered to high-fat diet induced obesity mice.The results indicated that T3 and M5 showed the highest hydroxyl radical and DPPH scavenging activities at a dose of 10 9 CFU/mL, with inhibition rates of 40.33, 41.11% and 40.76, 44.31%, respectively. The reducing activities of these two strains were also significantly higher than X12. Comparing with high-fat diet group, M5 and T3 have effectively controlled the body weight and hepatomegaly and significantly decreased the blood triglyceride and total cholesterol levels (p<0.05). Besides, M5 and T3 obvious improved antioxidative indexes including MDA levels, T-SOD, GSH-Px, CAT and T-AOC activities. Overall, M5 and T3 has a strong antioxidant activity in vitro and can improve oxidative damage and control body weight in mice, suggesting that it may be a potential anti-obesity and antioxidant probiotic.

Health promoting activities of probiotics

“…Till now, several probiotics have been proven to possess anti‐inflammatory and antioxidative activities in the obese individuals, which may suppress low‐grade chronic inflammation. In the previous study, three Lactobacillus strains could increase the activities of antioxidant enzymes but reduced MDA level in HFD‐fed mice (Song, Song, et al, ). Also, administration of L. plantarum LIP‐1 or L. sakei OK67 would reduce the relative abundance of LPS‐producing bacteria in HFD‐fed mice or rats (Lim, Jeong, Woo, Han, & Kim, ; Song et al, ).…”

“…Long‐term HFD may make host to produce much harmful ROS, leading to oxidative stress, and cellular oxidative damage. Moreover, malondialdehyde (MDA) content is also significantly increased during lipid peroxidation in the obese people (Song, Song, et al, ). Long‐term accumulation of ROS combined with decreased levels of antioxidant enzymes may result in normal cell apoptosis and low‐grade inflammation (Castaneda, Lee, Ho, & Huang, ; Han, Tang, et al, ).…”

“…Till now, accumulating evidences have suggested that probiotics possessed antioxidative and immune regulatory activities (Ewaschuk et al, ; Han, Kong, Chen, Sun, & Zhang, ; Smelt et al, ; Suo et al, ). Accordingly, several probiotics also improve the antioxidative defense and the immune system in the individuals with heavy mental exposure or metabolic disorders (Alard et al, ; Majlesi et al, ; Song, Song, et al, ; Zhai et al, ). Therefore, the aim of this review is to highlight the potential health promoting activities of probiotics based on their antioxidative, antimicrobial, and immune regulatory effects.…”

Strain-Specific Effects of Bifidobacterium longum on Hypercholesterolemic Rats and Potential Mechanisms

“…Our results show that the B. longum strain exerted a strain-specific effect on cholesterol lowering. The precise cholesterol-lowering mechanism of probiotics is not fully understood, and many hypotheses have been proposed, such as bile salt deconjugation [ 38 ], cholesterol assimilation [ 39 ] and antioxidant activity of probiotics [ 40 ]. In our study, after intragastric administration of B. longum CCFM 1077, the content of both total bile acids and total cholesterol in the rat feces increased significantly, B. longum I3 only affected the fecal bile acid content and B. longum J3 only affected the fecal cholesterol content, whereas B. longum B3 had no effects on these two contents in feces.…”

Understanding the Effects of Gut Microbiota Dysbiosis on Nonalcoholic Fatty Liver Disease and the Possible Probiotics Role: Recent Updates

“…Probiotic strains should live in duodenum environment, stimulate the immune system via the production of the antagonistic proteins (bacteriocins) to pathogens, produce short-chain fatty acids to improve the epithelial barrier and to increase the anti-inflammatory action and balance the gut microbiota 132 . Additionally, the candidate probiotics should have a protecting role in the host against the oxidative stress, anti-cholesterol, anti-obesity, and anti-diabetes 132 , 135 .…”