The Antioxidant Supplementation with <i>Filipendula ulmaria</i> Extract Attenuates the Systemic Adverse Effects of Nanosized Calcium Phosphates in Rats

Šćepanović, Radomir; Selaković, Dragica; Stanković, Jelena S. Katanić; Arsenijevic, Natalija; Andjelkovic, Marija; Milenkovic, Jovana; Milanović, Pavle; Vasovic, Miroslav; Jovičić, Nemanja; Rosić, Gvozden

doi:10.1155/2021/8207283

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…ALT is distributed in the cytoplasm, and the increase of ALT indicates that the membrane is damaged. AST is distributed in cytoplasm and mitochondria, and elevation suggests that the damage has reached organelle level [ 31 , 32 ]. If the liver is damaged or damaged, transaminases in liver cells enter the bloodstream, and elevated levels of ALT and AST in the blood signal liver disease.…”

Section: Discussionmentioning

confidence: 99%

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway

et al. 2022

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The body needs to generate heat to ensure basic life activities when exposed to cold temperatures. The liver, as the largest glycogen storage organ in the body and main heat-producing organ at rest, may play a role in chronic cold exposure. Recent studies suggested that pyroptosis plays a crucial role in liver diseases. However, the role of pyroptosis in cold stress-induced liver injury is not clear. Hence, in this study, we attempted to investigate the effects of chronic cold exposure on liver function, apoptosis, oxidative stress and inflammation in mice by establishing a mouse model of chronic cold exposure, and to investigate whether pyroptosis pathways are involved in the process of chronic cold exposure. In vivo, our results show that inflammatory cell infiltration and other pathological changes in liver cells and the activity of liver enzyme evidently increased in the serum and liver of cold-exposed mice, suggesting cold stress may result in liver injury. Remarkably, increased expression of heat shock protein 70 (HSP70) and HSP90 proteins proved the cold stress model is successfully constructed. Then, elevated levels of apoptosis, inflammation, oxidative stress and pyroptosis related proteins and mRNAs, such as cysteinyl aspartate specific proteinase-3 (Caspase-3), inducible nitric oxide synthase (iNOS), nuclear factor erythroid2-related factor 2 (Nrf2) and gasdermins D (GSDMD), confirmed that cold exposure activated apoptosis, oxidative stress and pyroptosis, and released inflammation cytokines. Meanwhile, in vitro, we got similar results as in vivo. Further, adding an NLR family pyrin domain containing 3 (NLRP3) inhibitors found that suppression expression of NLRP3 results in the essential proteins of pyroptosis and antioxidant evidently reduced, and adding GSDMD inhibitor found that suppression expression of GSDMD accompanies with the level of Nrf2 and heme oxygenase-1 (HO-1) obviously reduced. In summary, these findings provide a new understanding of the underlying mechanisms of the cold stress response, which can inform the development of new strategies to combat the effects of hypothermia.

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Section: Discussionmentioning

confidence: 99%

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway

et al. 2022

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Section: Discussionmentioning

confidence: 99%

“…It has been recently reported that the metallic compounds used as restorative materials can be also harmful by means of serious adverse effects [37,38]. Both metallic restorative compounds and nanoparticles may show numerous types of toxicities [37,38]. One of the main toxicity mechanisms is oxidative damage, and since curcumin (with confirmed antioxidant capacity [39], also in nanoformulation [40]), has been employed in this study, it appears reasonable to potentiate the beneficial role of curcumin in the prevention of those adverse effects.…”

Section: Discussionmentioning

confidence: 99%

The Preparation, the Physicochemical Assessment, and the Antimicrobial Action of Nanocurcumin‐Loaded Dental Temporary Restorative Material

Khosroshahi

Dizaj

Sharifi

et al. 2022

Journal of Nanomaterials

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Introduction. The aim of this study was to prepare a temporary restorative material containing curcumin nanocrystals and to evaluate the physicochemical properties and the antimicrobial action against Enterococcus faecalis (E. faecalis), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli). Materials and Methods. Dental temporary restorative material was physically mixed with curcumin nanocrystals at weight percentages of 80/20 and 70/30. The prepared materials were then subjected to the physicochemical tests: Fourier transmission infrared spectroscopy (FTIR) to investigate possible bonds, X-ray diffraction (XRD) method to test the crystallinity pattern, and scanning electron microscopy (SEM) method to study the morphology of the prepared materials. Besides, the release pattern of nanocurcumin from the temporary material was evaluated using drug dissolution USP apparatus II and UV spectrophotometer. For microbial evaluation, the disk diffusion method was used against the mentioned bacteria. Results. The results showed the homogenous mixing of nanocurcumin with the temporary restorative material. Nanocurcumin showed a two-stage release pattern from the temporary material, and both types of samples containing 20% and 30% nanocurcumin had antimicrobial action against all selected bacteria. Conclusion. Extensive cellular, animal, and clinical studies are needed to demonstrate the use of temporary restorative material containing antimicrobial nanoparticles.

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“…5 Electrons leak from the electron transport chain directly to oxygen, producing short-lived free radicals such as superoxide anion. 5 Scepanovic et al 6 had been reported that antioxidant addition with extract of Filipendula ulmaria reduces the toxic effects of nano-sized calcium phosphates in rats. Oxidative stress, apoptotic, and inflammatory response are the main mechanisms of toxicity of La 2 O 3 NPs.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cyto - and Genotoxic Influence of Lanthanum Dioxide Nanoparticles on Human Liver Cells

et al. 2022

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Inorganic nanoparticles are representing an emerging paradigm in molecular imaging probe design. We have determined lanthanum oxide nanoparticles (La2O3 NPs)-induced toxicity on human livers cells for 48 hrs. Before exposure to La2O3 NPs, the size and shape of NPs were confirmed by transmission electron microscope. It was found at 32 ±1.6 nm with a sheet-like morphological structure. The viability of CHANG and HuH-7 cells was reduced as the concentration of La2O3 NPs increased. HuH-7 cells were more sensitive than CHANG cells to La2O3 NPs. We observed production of intracellular ROS in HuH-7 cells was more than CHANG cells and the LPO level was more in CHANG cells than in HuH-7 cells at 50 μg/ml of La2O3 NPs. Glutathione was decreased and catalase was increased at 50 μg/ml of La2O3 NPs. More apoptotic and necrotic cells were observed at 300 μg/ml in HuH-7 cells FACS. DNA damage was observed by the SGCE test and more DNA damage was found in CHANG cells than HuH-7 cells at 300 μg/ml La2O3 NPs over 48 hrs. Thus, study warrants the application of La2O3 NPs in daily life and provides vital information about the toxicity of La2O3 NPs.

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The Antioxidant Supplementation with Filipendula ulmaria Extract Attenuates the Systemic Adverse Effects of Nanosized Calcium Phosphates in Rats

Cited by 5 publications

References 42 publications

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway

The Preparation, the Physicochemical Assessment, and the Antimicrobial Action of Nanocurcumin‐Loaded Dental Temporary Restorative Material

Evaluation of Cyto - and Genotoxic Influence of Lanthanum Dioxide Nanoparticles on Human Liver Cells

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